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Meeting the Challenge of Yellow Rust in Cereal Crops - ICARDA

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<strong>in</strong>fection <strong>of</strong> <strong>the</strong> pathogen for four years. To map <strong>the</strong> resistance gene(s) <strong>in</strong> <strong>the</strong><br />

genotype and transfer it <strong>in</strong>to common wheat, a cross was made between<br />

PI 480148 and susceptible common wheat genotype Avocet Susceptible.<br />

Resistant F 3 plants with 42 chromosomes were selected through Feulgensta<strong>in</strong><strong>in</strong>g<br />

<strong>of</strong> root tip cells and test<strong>in</strong>g with Pst races. When tested with Pst-100,<br />

<strong>the</strong> most predom<strong>in</strong>ant race <strong>in</strong> <strong>the</strong> USA for <strong>the</strong> last six years, 157 F 4 plants from<br />

a s<strong>in</strong>gle F 3 hexaploid plant segregated <strong>in</strong>to a 3:1 ratio for resistance vs<br />

susceptible plants, which identified a s<strong>in</strong>gle dom<strong>in</strong>ant gene from PI 480148.<br />

Us<strong>in</strong>g <strong>the</strong> F 3:4 population and <strong>the</strong> resistance gene-analogue polymorphism<br />

(RGAP) and simple sequence repeat (SSR) markers, <strong>the</strong> gene was mapped to<br />

<strong>the</strong> long arm <strong>of</strong> chromosome 2B. An RGAP marker and an SSR marker<br />

(Xwmc441) were closely l<strong>in</strong>ked to <strong>the</strong> resistance gene with a genetic distance <strong>of</strong><br />

2.7 and 5.6 cM, respectively. The effective resistance <strong>of</strong> <strong>the</strong> gene to an<br />

Australian isolate virulent to Yr5, which is located on 2BL and resistant to all<br />

Pst races <strong>in</strong> <strong>the</strong> USA so far, <strong>in</strong>dicated that <strong>the</strong> gene is different from Yr5 and<br />

should be a new and useful gene for resistance to stripe rust. Resistant<br />

common wheat l<strong>in</strong>es with plant types similar to Avocet Susceptible were<br />

selected for use <strong>in</strong> breed<strong>in</strong>g programmes to develop common wheat cultivars<br />

with <strong>the</strong> resistance gene.<br />

403<br />

Evaluation <strong>of</strong> germplasm for resistance to yellow rust <strong>in</strong><br />

Ankara <strong>in</strong> <strong>the</strong> period 2000–2008<br />

K. Akan, Z. Mert, L. Çet<strong>in</strong> and F. Düşünceli<br />

Central Research Institute for Field <strong>Crops</strong>, Ankara, Turkey<br />

<strong>Yellow</strong> rust caused by Pucc<strong>in</strong>ia striiformis f.sp. tritici is an important fungal<br />

disease <strong>of</strong> wheat. The disease caused significant economic losses <strong>in</strong> <strong>the</strong><br />

Cukurova region <strong>in</strong> 1995. Losses were estimated at 500 000 t. Use <strong>of</strong> genetic<br />

resistance and development <strong>of</strong> resistant cultivars are very important to control<br />

<strong>the</strong> disease. In this respect, we evaluated <strong>the</strong> resistance <strong>of</strong> adult plants <strong>of</strong><br />

genotypes from breed<strong>in</strong>g programme aga<strong>in</strong>st yellow rust under artificial<br />

<strong>in</strong>oculation <strong>in</strong> Ankara conditions dur<strong>in</strong>g <strong>the</strong> period 2000–2008. The experiment<br />

was conducted at <strong>the</strong> Central Research Institute for Field <strong>Crops</strong> (CRIFC) <strong>in</strong><br />

Ikizce-Haymana and Yenimahalle sites <strong>in</strong> Ankara, Turkey. Each genotype was<br />

planted as a s<strong>in</strong>gle row 1-m long. The trial was sown <strong>in</strong> October each year. The<br />

experiment was conducted under natural conditions, supplemented by mist<br />

irrigation. Cvs Little Club, Michigan Amber, Türkmen, Gerek 79, Seri 82 and<br />

Gün 91 were sown around <strong>the</strong> experimental field. In addition, Little Club was<br />

sown after every 10 entries and used as control and susceptible check <strong>in</strong> <strong>the</strong>

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