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Meeting the Challenge of Yellow Rust in Cereal Crops - ICARDA

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68<br />

Table 2. AFLP polymorphisms associated with resistant l<strong>in</strong>es (<strong>in</strong> coupl<strong>in</strong>g or repulsion)<br />

Primer<br />

comb<strong>in</strong>ation<br />

Polymorphism<br />

<strong>in</strong> LR resistant<br />

l<strong>in</strong>es<br />

Polymorphism<br />

<strong>in</strong> YR resistant<br />

l<strong>in</strong>es<br />

Polymorphism<br />

<strong>in</strong> susceptible<br />

l<strong>in</strong>es<br />

% association<br />

with LR<br />

resistance<br />

% association<br />

with YR<br />

resistance<br />

TCC/GTT 12/12 10/10 0/10 100 100<br />

TCG/GCA 12/12 10/10 0/10 100 100<br />

AAG/CGA* 2/11 0/10 11/11 91 100<br />

AAG/CTA* 10/11 10/10 1/10 90 95<br />

ACA/CGA 2/11 0/10 11/11 91 100<br />

NOTES: * denotes previously associated with Lr46/Yr29. LR = leaf rust; YR = yellow rust.<br />

Table 3. Summary <strong>of</strong> analysis <strong>of</strong> markers across entire Avocet "S"×Attila population<br />

to determ<strong>in</strong>e <strong>the</strong> level <strong>of</strong> association between markers and <strong>the</strong> amount each marker<br />

contributes to a reduction <strong>in</strong> disease severity <strong>of</strong> both leaf rust and yellow rust<br />

AAG/CTA<br />

(Lr46)<br />

AAG/CTA<br />

(Lr46)<br />

TCC/GTT TCG/GCA ACA/CGA<br />

%<br />

reduction<br />

LR severity<br />

%<br />

reduction<br />

YR severity<br />

100 79 82 82 36 20<br />

TCC/GTT – 100 98 86 51 29<br />

TCG/GCA – – 100 86 48 26<br />

ACA/CGA – – – 100 46 27<br />

NOTES: LR = leaf rust; YR = yellow rust.<br />

References<br />

Hois<strong>in</strong>gton D., Khairallah M. & Gonzalez-de-Leon D. 1994. Laboratory Protocols:<br />

CIMMYT Applied Molecular Genetics Laboratory. 2nd Ed. CIMMYT, Mexico, D.F.,<br />

Mexico.<br />

Johnson, R. 1988. Durable resistance to yellow (stripe) rust <strong>in</strong> wheat and its<br />

implications <strong>in</strong> plant breed<strong>in</strong>g. pp. 63-75, <strong>in</strong>: N.W. Simmonds and S. Rajaram<br />

(editors). Breed<strong>in</strong>g Strategies for Resistance to <strong>the</strong> <strong>Rust</strong>s <strong>of</strong> Wheat. CIMMYT, Mexico,<br />

D.F., Mexico.<br />

S<strong>in</strong>gh, R.P., Huerta-Esp<strong>in</strong>o, J. & Rajaram S. 2000. Achiev<strong>in</strong>g near-immunity to leaf<br />

and stripe rusts <strong>in</strong> wheat by comb<strong>in</strong><strong>in</strong>g slow-rust<strong>in</strong>g resistance genes. Acta<br />

Phytopathologica et Entomologica Hungarica, 35: 133–139.<br />

William, M., S<strong>in</strong>gh, R.P., Huerta-Esp<strong>in</strong>o, J., Ortiz Islas, S. & Hois<strong>in</strong>gton, D. 2002.<br />

Molecular marker mapp<strong>in</strong>g <strong>of</strong> leaf rust resistance gene Lr46 and its association with<br />

stripe rust resistance gene Yr29 <strong>in</strong> wheat. Genetics and Resistance, 93: 153–159.

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