few different pathogen stra<strong>in</strong>s. The application methods are also various among company, however the generalpractice are <strong>in</strong>clud<strong>in</strong>g <strong>in</strong>jection, bath<strong>in</strong>g, and feed<strong>in</strong>g. Age and size <strong>of</strong> fish also play a role <strong>in</strong> the response to thevacc<strong>in</strong>ation. Vacc<strong>in</strong>ation at the early stage <strong>of</strong> fish normally confers a better response to the vacc<strong>in</strong>e.Pr<strong>in</strong>ciple <strong>of</strong> vacc<strong>in</strong>ationVacc<strong>in</strong>ation should be conducted <strong>in</strong> the healthy fish and where the environment is free from the diseases. Fishshould not be subjected to any k<strong>in</strong>d <strong>of</strong> stress <strong>in</strong>clud<strong>in</strong>g diseases before vacc<strong>in</strong>ation. Th<strong>in</strong>gs to be consideredwhen apply a vacc<strong>in</strong>ation scheme <strong>in</strong>to the farm <strong>in</strong>clude fish age, vacc<strong>in</strong>ation method, time to vacc<strong>in</strong>ate, <strong>in</strong>tervalperiod between doses (if applicable) and withdrawal period before production harvest.Practical constra<strong>in</strong>tsThe ideal <strong>of</strong> vacc<strong>in</strong>ation is to provide effective protection to fish from the target disease throughout the productioncycle. However, most vacc<strong>in</strong>es have their limitation and vacc<strong>in</strong>ation procedure cannot avoid <strong>in</strong>troduc<strong>in</strong>g somecerta<strong>in</strong> degree <strong>of</strong> stress due to over crowd<strong>in</strong>g, handl<strong>in</strong>g, <strong>in</strong>sufficient oxygen supply and anesthetic effect.Sometime, protection period cannot be planed especially to non-seasonal diseases. Vacc<strong>in</strong>e cost and itsavailability are also major constra<strong>in</strong>t for the vacc<strong>in</strong>ation scheme <strong>of</strong> aquaculture.Evaluation <strong>of</strong> vacc<strong>in</strong>eEvaluation <strong>of</strong> the vacc<strong>in</strong>e efficacy is ma<strong>in</strong>ly done by the relative percent survival (RPS).us<strong>in</strong>g the follow<strong>in</strong>g formula;The calculation is madeRPS = 1- (vacc<strong>in</strong>ated fish mortality% / non-vacc<strong>in</strong>ated mortality%) x 100 %This method has a basel<strong>in</strong>e criteria that at least 25 fish should be used <strong>in</strong> duplicate for each test population, whilethe challenge should cause at least 60% mortality <strong>in</strong> control group on the same time scale <strong>of</strong> the natural disease.The cause <strong>of</strong> mortality should be determ<strong>in</strong>ed and non-specific <strong>in</strong>fection must not exceed 10% <strong>in</strong> any group andmortality rate <strong>of</strong> vacc<strong>in</strong>ated fish must be below 24% if the test is concerned to be positive (Ellis, 1988).Availability <strong>of</strong> fish vacc<strong>in</strong>eVacc<strong>in</strong>ation has been <strong>in</strong>tegrated <strong>in</strong>to the disease prevention strategies <strong>of</strong> the salmon and trout <strong>in</strong>dustries over adecade. S<strong>in</strong>ce these two fish species have a high marketable value, which a healthy fish stock justifies the pricepaid per vacc<strong>in</strong>e dose. Some other commercial vacc<strong>in</strong>es are also available such as for channel catfish, Europeanseabass and seabream, Japanese amberjack and yellowtail, Atlantic cod, and tilapia. Vacc<strong>in</strong>e aga<strong>in</strong>stStreptococcus <strong>in</strong>iae <strong>in</strong> Asian seabass is now available, whereas other bacterial and viral vacc<strong>in</strong>es are stilldeveloped.ConclusionDisease prevention strategies <strong>in</strong>volve with various factors <strong>in</strong>clud<strong>in</strong>g, species economy importance, f<strong>in</strong>ancialcircumstance and national regulation. Vacc<strong>in</strong>e is one <strong>of</strong> an ideal method as its benefit is long term last. However,availability <strong>of</strong> vacc<strong>in</strong>e particular to the Asian economic important fish species is rather limited. Cost <strong>of</strong> vacc<strong>in</strong>ationis also the ma<strong>in</strong> constra<strong>in</strong>t both the user and developer company. The health surveillance and good farm practiceseem to be better practical low cost measure to prevent and control the disease. However this may require atra<strong>in</strong><strong>in</strong>g, which <strong>in</strong>cludes from know-how level to pr<strong>of</strong>essional level. The scheme should be implemented <strong>in</strong>to thenational strategies and sufficient tra<strong>in</strong><strong>in</strong>g should be regularly provided to farmers.53
ReferencesAAHRI (1999) Diagnostic procedures for f<strong>in</strong>fish diseases. ISBN 974-7604-64-7.Bondad-Reantaso, M., McGladdery, S.E., East, I. & Subas<strong>in</strong>ghe, R.P. (2001). Asia diagnostic guide to aquaticanimal diseases. FAO Fisheries Technical Paper 402/2. 237pp.Cameron, A. (2002). Survey toolbox for aquatic animal diseases: a practical manual and s<strong>of</strong>tware package.ACIAR Monograph No. 94, 375pp.Ellis, A.E. (1988). Fish Vacc<strong>in</strong>ation. Academic Press: London, 255pp.54
- Page 1 and 2:
Training of TrainersProgramme3-7 Au
- Page 3 and 4: Table of Contents1. Preface 42. Sen
- Page 5 and 6: knowledge about the activities carr
- Page 7 and 8: iosphere, in that it is essentially
- Page 10 and 11: Importance in narrowing the supply
- Page 12 and 13: Figure 7: The trend in aquaculture
- Page 14: aquaculture has been a success thus
- Page 17 and 18: pathogen transfer is generally cons
- Page 19 and 20: • It highlights the importance a
- Page 21 and 22: particular animal are identified, t
- Page 23: Knowledge at the bottom of the pyra
- Page 26 and 27: farmer innovation process. In this
- Page 28 and 29: • Rapport building• Working str
- Page 30 and 31: shrimp farming is undertaken in the
- Page 32 and 33: Reintjes, C and Hiemstra, W. 1989 F
- Page 34 and 35: • An increase in reliable product
- Page 36 and 37: Principles of AACC are to: 1) Facil
- Page 38 and 39: Maintaining genetic quality of fish
- Page 40 and 41: Where F is the percent increase in
- Page 42 and 43: o G=(0.95) 1/10G =0.994883803Figure
- Page 44 and 45: Aquaculture extension and training
- Page 46 and 47: ResearchExtensionFarmersFlow of inf
- Page 48 and 49: view of outside world than before a
- Page 50 and 51: Along with fish sample, the support
- Page 52 and 53: Fungal examinationFungal infection
- Page 56 and 57: Major finfish diseases in Asia and
- Page 58 and 59: A great number and diversity of ani
- Page 60 and 61: • -oxidase test positive• -resi
- Page 62 and 63: ReferencesAustin, B. and D. Austin.
- Page 64 and 65: Accessing better markets-improving
- Page 66 and 67: Owing to its importance in generati
- Page 68 and 69: adopt standards for responsible shr
- Page 70: Tokrisna R, Benheam W., 1995. Gain
- Page 73 and 74: of the temperature rise that were d
- Page 75 and 76: Principles of developing, validatin
- Page 77 and 78: such as determinants, exposures and
- Page 79 and 80: How to promote adoption of BMPs?Pro
- Page 81 and 82: economic sustainability. BMPs need
- Page 83 and 84: Once you understand your stakeholde
- Page 85 and 86: As conventional approaches such as
- Page 87 and 88: Printed publicationsPrinted publica
- Page 89 and 90: Set up a blog or website for your w
- Page 91 and 92: If you have a website, all you need
- Page 93 and 94: ought about increased profits among
- Page 95 and 96: • It is a farming system the prod
- Page 97 and 98: Annex 1. The area surveyed for obta
- Page 99 and 100: contribute substantially to maintai
- Page 101 and 102: seawater, but in fresh water its le
- Page 103 and 104: type of ingredients used, since som
- Page 105 and 106:
This is a new approach used to buil
- Page 107 and 108:
New, M.B., A. G. J. Tacon and I. Cs
- Page 109 and 110:
later stages it has become a much m
- Page 111 and 112:
c. Improved information exchange an
- Page 113 and 114:
implemented by both societies. Inte
- Page 115 and 116:
With better informed farmers, the s
- Page 117 and 118:
According to FAO, it is estimated t
- Page 119 and 120:
Farmer organization as models for p
- Page 121 and 122:
NaCSA disseminates BMPs mainly thro
- Page 123 and 124:
1. Drain the pond water completely
- Page 125 and 126:
Seed transportation and Stocking:
- Page 127 and 128:
7. Presently 100% of the society po
- Page 129 and 130:
would help in sustaining shrimp sec
- Page 131 and 132:
Stress brought about by the capture
- Page 133 and 134:
Enhance women participation in aqua
- Page 135 and 136:
women in aquaculture and identify a
- Page 137 and 138:
major percentage of staff, the need
- Page 139 and 140:
having more self confidence through
- Page 141 and 142:
Overall status of men and women in
- Page 143 and 144:
Compliance to international standar
- Page 145 and 146:
(ii) to protect human or animal lif
- Page 147 and 148:
c) FAO/WHO Codex Alimentarius Commi
- Page 149 and 150:
Annex 1: List of participantsCountr
- Page 151 and 152:
Annex 2: AgendaDate Time Presentati
- Page 153:
Annex 3: List of resource persons1.