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Post harvest diseases fruits and vegetables - Xavier University ...

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FREEDOM PALESTINE FREEDOM PALESTINE FREEDOM PALESTINE<br />

Means for Maintaining Host Resistance 139<br />

effect on fungal development in vitro, or in vivo on inoculated <strong>fruits</strong>, <strong>and</strong><br />

its effect on decay development was attributed to the enhancement of<br />

fruit resistance. A pre-<strong>harvest</strong> combination of GA3 <strong>and</strong> iprodione<br />

treatments may further reduce the percentage of <strong>fruits</strong> rendered<br />

unmarketable by the black spot disease (Perez et al., 1995).<br />

Retardation of senescence by growth regulators has also been reported<br />

for leafy <strong>vegetables</strong>. Fresh herbs are prone to accelerated senescence<br />

because of their high rate of metabolism, which is further increased<br />

following <strong>harvest</strong>ing <strong>and</strong> h<strong>and</strong>ling procedures. Their accelerated<br />

senescence is expressed in decreased levels of RNA, proteins, fats,<br />

carbohydrates, organic acids <strong>and</strong> vitamins, <strong>and</strong> increased enzymatic<br />

activity of RNase, protease, lypase <strong>and</strong> other hydrolytic enzymes, as well<br />

as those associated with fat oxidation. These changes are accompanied by<br />

chloroplast destruction <strong>and</strong> chlorophyll decomposition, resulting in a<br />

rapid yellowing of the leaves (Aharoni, 1994). During <strong>harvest</strong>ing, grading<br />

<strong>and</strong> packaging, when the leaves lose water because of evaporation, <strong>and</strong><br />

suffer from mechanical injury, the senescence process is further<br />

enhanced <strong>and</strong> is accompanied by increased ethylene <strong>and</strong> respiration<br />

levels. The endogenous ethylene continues to accelerate chlorophyll<br />

destruction <strong>and</strong> leaf senescence (Meir et al., 1992). Along with their<br />

aging, the leaves lose their natural resistance to pathogens <strong>and</strong> tend to<br />

rot. In many cases, the senescence process is controlled by plant<br />

hormones; it is accompanied by decreases in the levels of<br />

growth-stimulating hormones - gibberelin, cytokinin <strong>and</strong> auxin, <strong>and</strong><br />

increases in those of growth-inhibiting hormones - ethylene <strong>and</strong> abscissic<br />

acid (ABA). It is not surprising that application of synthetic hormones -<br />

gibberelin, cytokinin <strong>and</strong> a very low concentration of auxin - was very<br />

efficient in delaying leaf senescence, while application of ABA or ethylene<br />

accelerated it (Aharoni <strong>and</strong> Richmond, 1978). Delay of senescence by<br />

gibberelin <strong>and</strong> cytokinin is attributed to their antagonistic effects on<br />

ethylene <strong>and</strong> to their ability to neutralize the stimulation of chlorophyll<br />

destruction caused by ethylene (Aharoni, 1989).<br />

The delay of aging conferred by GA3 has also been found in several<br />

fresh herbs which tend to yellow, such as chives, dill, chervil, cori<strong>and</strong>er<br />

<strong>and</strong> parsley (Aharoni, 1994). In a laboratory experiment with chives, a<br />

dip in GA3 solution (10 ppm) was found to prevent the accelerated<br />

respiration caused by the cutting stress, <strong>and</strong> then to prevent the<br />

climacteric respiration associated with leaf senescence. A marked effect<br />

on respiration was achieved by spraying chives in the greenhouse, close<br />

to <strong>harvest</strong>ing. Such a treatment delays chlorophyll destruction, retards<br />

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