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Post harvest diseases fruits and vegetables - Xavier University ...

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FREEDOM PALESTINE FREEDOM PALESTINE FREEDOM PALESTINE<br />

Chemical Control 151<br />

abundance of pathogenic spores, which may originate from infected<br />

<strong>fruits</strong> <strong>and</strong> <strong>vegetables</strong> covered with spores, or from plant remnants in<br />

the packinghouse or its surroundings, which serve as substrates for<br />

many pathogenic fungi. During the citrus packing season, spores of<br />

Penicillium digitatum <strong>and</strong> P. italicum form the most conspicuous<br />

components of the spore population in packing houses <strong>and</strong> their vicinity.<br />

Fruit containers, the equipment in the packinghouse, as well as the<br />

workers' h<strong>and</strong>s <strong>and</strong> tools, all bear pathogenic fungal spores during this<br />

season. Fresh <strong>fruits</strong> arriving at the packinghouse may, therefore, come<br />

into contact with pathogenic spores from any of these sources<br />

(Barkai-Golan, 1966).<br />

Many <strong>fruits</strong> <strong>and</strong> <strong>vegetables</strong> have to be cleaned <strong>and</strong> washed on arrival<br />

at the packinghouse, in order to remove soil particles, dust or other<br />

contaminants. Water that does not contain disinfectants becomes heavily<br />

contaminated by fungal spores <strong>and</strong> bacterial cells <strong>and</strong> may infect<br />

products treated with non-recirculated water. Chlorine is the principal<br />

disinfectant used to sanitize wash water in packinghouses. Solutions of<br />

hypochlorous acid <strong>and</strong> its salts (sodium or calcium hypochlorite) are the<br />

most effective <strong>and</strong> economical agents available for destroying<br />

microorganisms in water <strong>and</strong> they have been applied widely to reduce<br />

bacterial contamination of the water used to wash or to hydrocool <strong>fruits</strong><br />

<strong>and</strong> <strong>vegetables</strong> (Eckert <strong>and</strong> Sommer, 1967). Elemental chlorine (CI2)<br />

reacts with water to form hypochlorous acid (HOCI), hydrogen ion, <strong>and</strong><br />

chloride ion:<br />

CI2+H2O '^—^ HOCl + C1-+H+<br />

Hypochlorous acid is a weak acid (pka=7.46), which dissociates to<br />

hypochlorite ion (OCl") <strong>and</strong> H+:<br />

HOCl ^—^ 0C1-+H+<br />

Hypochlorous acid <strong>and</strong> OCl" are in equilibrium in the solution, but the<br />

antimicrobial effectiveness of the chlorine is correlated with the<br />

concentration of the hypochlorous acid in the solution (Bartz <strong>and</strong> Eckert,<br />

1987). The relative concentration of the hypochlorous acid <strong>and</strong> its<br />

antimicrobial activity depend on the pH of the solution (Fig. 26).<br />

If the initial concentration of hypochlorous acid is not sufficient to<br />

kill microbes on contact, the hypochlorous acid formed by conversion<br />

from hypochlorite ion should become involved, provided the exposure<br />

period is long enough <strong>and</strong> the pH is not too high to inhibit the<br />

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