Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
3. Environmental Fate and Pathways Substance ID: 71–43–2
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Type: aerobic
Inoculum: activated sludge, domestic, non–adapted
Concentration: 17 mg/l related to Test substance
Degradation: 53 – 100 % after 28 day(s)
Result: other: not valid because of over 20 % difference between
replicates of the test.
Method: OECD Guide–line 301 F "Ready Biodegradability: Manometric
Respirometry Test"
Year: 1999
GLP: yes
Test substance: no data
Method: The test was run with three parallel vessels.
Result: Benzene in the tested concentration was inhibitory over the
first week of incubation, with oxygen uptake in the test
flasks being lower than in the blanks.
The lag periods in the three test flasks ranged from 4 to 7
days.
At the end of the 10–day window a degradation of 58 %, 72 %
and 87 % was measured.
After 28 days biodegradation of benzene (measured as ThOD)
was 53 %, 80 % and 102 % in the three parallel vessels.
As the biodegradation of the replicates differ by more than
20 % the test is regarded as not valid.
Source: German Rapporteur
Reliability: (3) invalid
Flag: Risk Assessment
16–MAR–2004 (1032)
Type: aerobic
Inoculum: activated sludge, domestic, non–adapted
Concentration: 17 mg/l related to Test substance
Degradation: 63 % after 28 day(s)
Result: other: not valid because of over 20 % difference between
replicates of the test.
Method: OECD Guide–line 301 F "Ready Biodegradability: Manometric
Respirometry Test"
Year: 2001
GLP: yes
Test substance: other TS: benzene, purity > 99 %
Result: The average percent biodegradability of benzene was
determined to be 63 % (n=3) over a 28 day testing period (54%,
72 % and 63 % in the single replicates).
Benzene was not inhibitory to the test systems based on the
results of the toxicity control experiments.
As the biodegradation of the replicates differ by more than
20 % the test is regarded as not valid.
Test condition: The aqueous aerobic biodegradability potential of benzene
was determined using the OECD (301F) manometric respirometry
test method. Biodegradability was determined by measuring
the oxygen consumption in a test medium with trace nutrients
and inoculated with activated sludge supernatant
(unacclimated). Benzene was evaluated at a
concentration of 17 mg/L (carried out in triplicate test
Appendix D: Benzene SIDS Dossier
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