Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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ten fish were exposed to benzene in either soft or hard
freshwater at 25 degree C. The hardness was measured to be
20 and 360 mg/l (EDTA) respectively, alkalinity 18 and 300
mg/l respectively, pH 7.5–8.2 and dissolved oxygen 7.8 mg/l.
Test substance: Reagent grade benzene tested.
Reliability: (3) invalid
Flag: Risk Assessment
17–MAR–2004 (895)
Type: static
Species: Poecilia reticulata (Fish, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no
LC50: = 36.6 –
Method: other
Year: 1966
GLP: no data
Test substance: other TS: reagent grade
Remark: Concentration of benzene was not checked analytically in an
open, static test system (wide–mouth bottles).
Test procedure in accordance with national standard methods
with acceptable restrictions
Synonym for the species tested: Poecilia reticulata.
LC50 confidence limits = 28.63–54.33 mg/l.
Further tests gave 48– and 96–hour LC50 values of 36.60 mg/l
(confidence limits 28.63–54.33 mg/l).
Source: German Rapporteur
Test condition: The method of the APHA. Standard Methods for the Examination
of Water and Wastewater. 11th Edition. American Public
Health Association, New York, 1960 was followed. Groups of
ten fish were exposed to benzene at 25 degree C in
freshwater (hardness 20 mg/l (EDTA), alkalinity 18 mg/l, pH
7.5 and dissolved oxygen 7.8 mg/l).
Test substance: Reagent grade benzene tested.
Reliability: (3) invalid
Flag: Risk Assessment
17–MAR–2004 (895)
Appendix D: Benzene SIDS Dossier
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