Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Species: Chlamydomonas sp. (Algae)
Endpoint: other
Exposure period: 3 hour(s)
Unit: mg/l Analytical monitoring: no data
EC50: = 461 –
Method: other
Year: 1980
GLP: no data
Test substance: no data
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Exponential phase Chlamydomonas angulosa (green algae, 680
Indiana collection) cells (5 x 10+4 cells/ml), grown axenic
in Bolds Basal Medium at a 12 h dark/12 h light cyclus, were
exposed in glass stoppered flasks under static conditions at
19 degree C and pH 6.5. A dark set of controls was also
run. Benzene was added from saturated stock solutions in
medium. Radiolabelled carbon dioxide uptake (1.25 uCi/100
ml) from NaHCO3 (purity not specified) was measured as a
sign of effects on photosynthesis. This may indicate a
biomass effect but no details were given.
Test substance: No information on the purity of the test substance was
given.
Reliability: (2) valid with restrictions
Flag: Risk Assessment
17–MAR–2004 (545)
Species: Chlorella vulgaris (Algae)
Endpoint: growth rate
Exposure period: 24 hour(s)
Unit: mg/l Analytical monitoring: no data
EC50: = 525 –
Method: other
Year: 1975
GLP: no data
Test substance: no data
Remark: In the study, the effects of benzene on growth of the
unicellular algae Chlorella vulgaris (culture no. 29,
Indiana University Culture Collection of Algae) was
studied.
Significant methodological deficiencies
The concentration of benzene in the culture medium at 20
degree C was calculated from reported solubility data of a
saturated benzene solution in pure water at 25 degree C.
Actual benzene concentrations were not measured.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: 30000–100000 cells were incubated with various
concentrations of benzene in 50 ml Bolds Basal Medium at
18–22 degree C in cotton–plugged 125 ml Erlenmeyer flasks
under a 12 h light/12 h dark cycle. Growth was recorded by
determination of cell number.
Effective concentrations refer to nominal concentrations at
Appendix D: Benzene SIDS Dossier
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