Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Species: other aquatic crustacea: Cancer magister
Endpoint: other
Exposure period: 20 day(s)
Unit: mg/l Analytical monitoring: yes
NOEC: ca. .17 –
LOEC: ca. 1.1 –
Method: other
Year: 1977
GLP: no data
Test substance: no data
Method: The concentration of benzene in the test solutions was
measured by UV–spectrophotometry (at 255 nm).
Result: Benzene had little effect on the duration of the larval
stages and had no effect on the size of surviving larvae.
At the lowest concentration used, benzene had no effect on
survival. At the other two concentrations used, benzene led
to an accelerated mortality rate compared to untreated
controls. After 10 days of exposure to the highest
concentration of benzene (6.5–7 mg/l), most larvae died.
With 1.1–1.2 mg/l, mortality rate was less but most larvae
died before day 20 of exposure.
Control mortality increased steadily during the two tests.
At the end of the experiments control survival was only 10 %
in the fist assay and about 35 % in the second. From the
poor control survival it can be concluded, that the test
animals were in poor health and/or stressed by test factors
other than the benzene exposure. Therefore, the result of
this study cannot be used for risk assessment purposes.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Larval stages of the Dungeness crab (Cancer magister), were
continuously exposed after hatching in flowing water
laboratory culture systems to benzene for up to 60 days as
follows:
Filtered sterile seawater,saturated with air, salinity:
29–34 g/l, temperature: 10.5–14.2 degree C, photoperiod: 11h
dark/13h light, 3 benzene concentrations (0.17–0.18 mg/l,
1.1–1.2 mg/l, and 6.5–7.0 mg/l) and control.
In each test, 25 larvae/culture container were used, a total
of 300 in controls and 100 in each exposure treatment.
Larvae were fed brine shrimp during exposure and were
transferred to clean culture containers three times a week.
Effects on survival, duration of larval development and size
were employed as indicators of toxic effects.
Reliability: (3) invalid
Flag: Risk Assessment
17–MAR–2004 (172)
Appendix D: Benzene SIDS Dossier
– 306/957 –