Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
______________________________________________________________________________
4 h after treatment of HL60 cells with 50, 75 and 100 microM
MUC. No increases in DNAPC levels were measured in HL60
cells 4 h after treatment with the MUC metabolites
6–hydroxy–trans,trans–2,4–hexadienal (HO–M–CHO),
6–oxo–trans,trans–2,4–hexadienoic acid (CHO–M–COOH), or
trans,trans–muconic acid (HOOC–M–COOH), each at 100 microM.
Significant increases in DNAPC levels were obsd. 4 h after
treatment with 500 and 1000 microM HO–M–CHO, but not
CHO–M–COOH. No effect on DNAPC levels was obsd. 4 h after
treatment with 100 microM for trans,trans–2,4–hexadienal,
trans–2–hexenal, hexanal, trans,trans–2,4–hexadiene,
glutaraldehyde, or acrolein. DNAPC induced by MUC and
HO–M–CHO may be cytotoxic lesions, as increases in DNAPC
levels by these compds. correlated with decreases in cell
viability. Except for acrolein, compds. not inducing DNAPC
at 100 microM also did not affect cell viability. These
studies suggest that both aldehydic carbons of MUC
contribute to DNAPC induction, and that the presence of
alpha,beta–unsatd. double bonds conjugated with the aldehyde
groups increases the ability of MUC to induce DNAPC relative
to the satd. dialdehyde glutaraldehyde.
Source: ExxonMobil Biomedical Sciences Inc. Annadale, New Jersey
14–FEB–2002 (1026)
Type: other: Benzene percutaneous absorption: dermal exposure
relative to other benzene sources.
Remark: Skin is one of several exposure routes whereby benzene, a
widely distributed environmental contaminant that causes
leukemia, enters the body, so accurate predictions of its
percutaneous absorption are important for risk assessment.
Determining benzene’s skin–exposure dose and subsequent
absorption is difficult because it has a low boiling point
and exists as both liquid and vapor. Industrial and
environmental benzene is present as a contaminant in other
vehicles/solvents, and its percutaneous absorption is in
part dependent upon co–solvent volatility. Co–solvents such
as benzene in toluene rapidly evaporate from skin, whereas
benzene contaminant in water is retained on skin longer due
to water’s lower volatility. Co–solvents can also affect
benzene–skin partition coefficients; thus, permeability
coefficients and percentage doses absorbed can vary
many–fold. The exposure situation will determine
percutaneous absorption, which, if low, can be overwhelmed
by benzene intake from the food we eat and the air we
breathe.
Source: EXXON Biomedical Sciences East Millstone, NJ
Reliability: (1) valid without restriction
21–JUL–2000 (1246)
Appendix D: Benzene SIDS Dossier
– 792/957 –