Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Type: aquatic
Species: Tetrahymena pyriformis (Protozoa)
Exposure period: 24 hour(s)
Unit: mg/l Analytical monitoring: no data
EC0: 391 –
Method: other
Year: 1983
GLP: no data
Test substance: other TS
Result: Value reported is the acute toxicity threshold level, i.e.
the intermediate concentration between the lowest and
highest concentration causing cell death and permitting
replication, respectively.
In the study, the value is given as 5010 mmol/m3.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: The ciliate T. elliotti (=T. pyriformis; strain L1630/1C)
was used. Ca. 500 cells were treated with different
concentrations of benzene in a 10 ml sterile, gas tight
syringe to avoid volatilisation of benzene. After 24 h, cell
death was recorded using cessation of ciliary movement as
criterion.
Benzene solutions were prepared from a saturated stock
solution, which concentration was determined by GC analysis.
Test substance: Purity: 99%
Reliability: (2) valid with restrictions
Flag: Risk Assessment
17–MAR–2004 (954)
Species: Entosiphon sulcatum (Protozoa)
Exposure period: 72 hour(s)
Unit: mg/l Analytical monitoring: no data
EC0: > 700 –
Method: other
Year: 1980
GLP: no data
Test substance: no data
Remark: Endpoint: toxicity threshold (TGK) for cell multiplication.
No measurement of actual concentrations; no data about purity
of test substance.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: 20 ml test solution (Entosiphon in defined medium with
appropriate concentration of test substance) is incubated at
pH 6.9 and 25 degree C in metal–cap stoppered 300 ml
Erlenmeyer flasks for 72 h under static conditions.
The number of cells is determined by means of a cell counter.
Reliability: (2) valid with restrictions
17–MAR–2004 (148)
Appendix D: Benzene SIDS Dossier
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