Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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by in vivo chlorophyll fluorescence on the 2nd or 3rd day
of log. growth.
Test substance: Spectro–grade benzene was tested.
Reliability: (2) valid with restrictions
17–MAR–2004 (313)
Species: other algae: Cricosphaera carterae (coccolithophorid)
Endpoint: growth rate
Unit: mg/l Analytical monitoring: no data
EC50: > 100 –
EC : > 10 –
Method: other
Year: 1975
GLP: no data
Test substance: other TS
Remark: Growth was not inhibited up to 10 mg/l. At 100 mg/l, the
highest concentration tested, benzene inhibited growth to
about 70% of controls.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Axenic cultures of Cricosphaera carterae (Clone, 156,
Plymouth) were incubated under the following conditions:
12h dark/ 12h white light (ca. 4000 uW/cm2 at 380–700 nm)
125 ml flasks with rubber stopper, 3 flasks/concentration.
50 ml enriched filtered seawater
temperature: 18 degree C.
Cell density not specified, but such that growth was not
limited due to CO2–shortage.
Benzene concentration was monitored by gas chromatography.
Values given refer to theoretical values, not measured
concentrations, which, after 3 days of culture, dropped to
ca. 20–50% of the calculated initial amount.
Growth was measured by direct cell count (hemacytometer) and
by in vivo chlorophyll fluorescence on the 2nd or 3rd day
of log. growth.
Test substance: Spectro–grade benzene was tested.
Reliability: (2) valid with restrictions
17–MAR–2004 (313)
Appendix D: Benzene SIDS Dossier
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