Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
3. Environmental Fate and Pathways Substance ID: 71–43–2
______________________________________________________________________________
Type: aerobic
Inoculum: other
Concentration: 1 mg/l related to Test substance
Degradation: > 99 % after 16 day(s)
Method: other
Year: 1985
GLP: no data
Test substance: no data
Method: Removal of benzene in groundwater samples from Gainesville,
Florida (drawn from a 9 m deep shallow).
Batch culture experiments according to Bouwer et al.,
Environ. Sci. Technol. 15, 596–599. Elimination of test
substance monitored by GC–MS. Sterile control.
Result: After an acclimatization phase of 8 days and a period of slow
degradation of 4 days a complete primary degradation of
benzene was achieved between day 12 and day 16. No change in
benzene concentration in sterile control indicates biological
transformation of TS.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Incubation at 20 degree C in the dark; pH 5.3
Reliability: (2) valid with restrictions
No standard test procedure, but in accordance with generally
Flag: Risk Assessment
02–SEP–2003 (292)
Type: aerobic
Inoculum: other
Method: other
Year: 1981
GLP: no data
Test substance: no data
Method: The static–culture flask–screening procedure of Bunch R.L. &
Chambers C.W. J. Wat. Pollut. Control Fed. 39, 181 (1967)
modified by Tabak H.H. & Hannah S. Static Procedure for
Biodegradability Determination. Internal EPA Report, Mun.
envir. Res. Lab., Cincinnati, Ohio (1979) was followed.
Wastewater from domestic sewage was used as inoculum.
Benzene was prepared in deionized distilled water as a 10%
solution to give concentrations of 5 and 10 mg benzene/l.
The solutions were incubated with prechilled dilution water
containing 5 mg yeast extract/l, and also with settled
domestic wastewater inoculum. The flasks were then incubated
for 7 days in glass stoppered bottles (to minimize
volatilization) and three weekly subcultures were taken
(totalling 28 days of incubation). Duplicate samples at the
beginning of each incubation period and triplicate samples
at the end of each 7–day period were subjected to GC, total
organic carbon (TOC) and dissolved organic carbon (DOC)
analyses. The minimum sensitivity of the GC procedures used
was about 0.1 mg/l.
Remark: Modification of standard test procedure; method in
accordance with generally accepted standards and described
Appendix D: Benzene SIDS Dossier
– 207/957 –