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Appendix D - Dossier (PDF) - Tera

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date: 20–JUL–2005<br />

3. Environmental Fate and Pathways Substance ID: 71–43–2<br />

______________________________________________________________________________<br />

Type: aerobic<br />

Inoculum: other<br />

Concentration: 3 mg/l related to Test substance<br />

Degradation: = 29 % after 20 day(s)<br />

Result: other<br />

Method: other<br />

Year: 1974<br />

GLP: no data<br />

Test substance: no data<br />

Result: 24, 27, 24 and 29% bio–oxidation was recorded after 5, 10,<br />

15 and 20 days respectively indicating that benzene is<br />

biodegradable.<br />

Source: BP Chemicals Ltd LONDON<br />

German Rapporteur<br />

Test condition: The method of the APHA. Standard Methods for the Examination<br />

of Water and Wastewater. 13th Edition. American Public<br />

Health Association, New York, 1971 was followed. Wastewater<br />

from domestic sewage was used as inoculum. Settled domestic<br />

wastewater was filtered through glass wool and added as seed<br />

material. Aerated dilution water (freshwater) containing<br />

minerals and buffer was added to the BOD bottles followed by<br />

small aliquots of benzene, at concentrations of 3, 7 and 10<br />

mg/l, taken from 0.1% stock solutions. At least two of the<br />

concentrations were tested in duplicate. Dissolved oxygen<br />

was monitored periodically. Samples were analysed routinely<br />

for ammonia, nitrogen and organic nitrogen which may result<br />

in an oxygen demand.<br />

Reliability: (2) valid with restrictions<br />

guideline study without detailed documentation<br />

Flag: Risk Assessment<br />

02–SEP–2003 (910)<br />

Type: aerobic<br />

Inoculum: other<br />

Concentration: 480 µg/l related to Test substance<br />

Degradation: = 54.6 % after 48 hour(s)<br />

Result: other<br />

Method: other<br />

GLP: no data<br />

Test substance: no data<br />

Method: Removal of benzene in gasoline–contaminated groundwater<br />

collected from a monitoring well in Los Angeles by the<br />

autochthonic, petroleum degrading microflora was studied.<br />

Influence of additional nitrogen and from contaminated soil<br />

enriched microbial inocula to the test culture was<br />

investigated.<br />

Elimination determined, amount of stripped or adsorbed test<br />

substance not measured.<br />

Remark: Longer incubation than 20 hours did not result in further<br />

elimination, probably due to 35 ug/l benzene being an<br />

insufficient carbon source to sustain the microbial<br />

population.<br />

Result: (I) and (II):

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