Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
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mg/kg bw/day.
The number of polychromatic erythrocytes was reduced at 2198
mg/kg bw/day and, therefore, the reading of smears was
unsatisfactory. Male mice were more sensitive to
micronucleus induction than the females. These
investigators have previously reported positive micronuclei
tests in mice (Siou G. & Conan L. Cah. Notes Doc. 89,
443–444, 1977; Siou G. & Sourdeix D. ibid. 101, 531–534,
1980).
Source: Deutsche Shell Chemie GmbH Eschborn
Test substance: "Chemically pure" benzene tested.
06–JAN–1997 (1042)
Type: Micronucleus assay
Species: mouse Sex: male/female
Strain: other: Swiss CD–1
Route of admin.: gavage
Exposure period: 3 days
Doses: 0, 0.1, 0.5, 1 ml/kg bw/day (converts to 0, 88, 440, 880 mg/kg
bw/day)
Method: other: nearly in compliance with OECD Guideline 474
GLP: no data
Test substance: other TS
Remark: Benzene diluted in corn oil. Control animals received corn
oil only. Groups of five males and five females were
exposed at each dose level. The animals were sacrificed 4
hours after the final dose (2 h after colchicine treatment,
assay combined with metaphase analysis), the femurs removed
and bone marrow used for cytogenetic preparations. 5 male
and 5 female mice per group.
Reliability: 2 (valid with restriction)
Comparable to guideline study with acceptable restrictions
no positive control, no toxicity data, one sampling time
Result: Significant increase in frequency of micronuclei was seen in
the in the mid– and high–dosed male and female mice.
Source: Deutsche Shell Chemie GmbH Eschborn
Test substance: >99% pure.
06–JAN–1997 (771)
Type: Micronucleus assay
Species: mouse Sex: male/female
Strain: CD–1
Route of admin.: unspecified
Exposure period: 0, 15, 30, 60, 90 days
Doses: 1, 10, 30, 300 pmm
Method: other: see ref.
Year: 1985
GLP: no data
Test substance: as prescribed by 1.1 – 1.4
Result: Exposure to 3000 ppm benzene caused a significant increase
in micronucleated polychromatic and monochromatic
erytrocytes in male and female mice at all simples times.
Source: REPSOL PETROLEO, S.A. MADRID
Appendix D: Benzene SIDS Dossier
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