Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Type: static
Species: Gasterosteus aculeatus (Fish, estuary, marine)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: yes
LC50: = 21.8 –
Method: other
Year: 1979
GLP: no data
Test substance: no data
Result: LC50 95% confidence limits = 19–25 mg/l.
Most deaths occurred during the first 12 h of exposure.
Effect values are related to initial nominal concentrations.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Three–year–old adult three spine sticklebacks (length ca. 55
mm, 12 fish/concentration) were exposed in freshwater at 8
degree C. Solutions were aerated during the final 48 h to
ensure dissolved oxygen content >85% saturation.
Concentrations of benzene as monitored by
UV–spectrophotometry (at 254 nm) decreased to ca. 75% of
initial value in 24 h and to ca. 10% after 96 h,
respectively.
Test substance: purity not specified
Reliability: (2) valid with restrictions
Flag: Risk Assessment
17–MAR–2004 (787)
Type: static
Species: Ictalurus punctatus (Fish, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: no data
LC50: = 425 –
Method: other
GLP: no data
Test substance: no data
Remark: LC50 confidence limits = 357–505 mg/l.
Further 96h LC50 values (confidence limit) given:
rainbow trout (appr. 2.4g), 12 degree C: 9.2 mg/l (7.2–11.7)
Bluegill sunfish (appr. 0.2 g), 22 degree C: 100 mg/l
(70–142).
Secondary literature
Data from handbook with compilation of results
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Fish (approx. 0.1 g) exposed in freshwater aerated prior to
use at 22 degree C to benzene. Water hardness was 40–50 mg/l
as CaCO3, alkalinity was 30–35 mg/l as CaCO3 and pH was
7.2–7.5.
Reliability: (4) not assignable
02–SEP–2003 (585)
Appendix D: Benzene SIDS Dossier
– 246/957 –