Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
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magnitude of the response of bone marrow cellular
populations, as determined by different cytogentic end
points in male and female mice exposed to benzene or to
phenobarbital and benzene, suggest not only that a
metabolite of benzene is responsible for theobserved
effects, but that different metabolites may be involved in
different end points.
Source: Deutsche Shell Chemie GmbH Eschborn
06–JAN–1997 (1139)
Type: Somatic mutation assay
Species: Drosophila melanogaster Sex: male/female
Strain: other
Route of admin.: oral feed
Exposure period: up to 96 hours
Doses: 0.88, 4.4, 8.8 mg/ml
Method: other
Year: 1983
GLP: no data
Test substance: other TS
Remark: The method of Wurgler F.E. et al. Mutation Res. 122,
321–328, 1983 was followed. Benzene was administered to
mei–9 and +/mei–9 strain Drosophila as a solution in 5%
Tween 80 and 5% ethanol. Larvae aged 48 or 72 hours were
exposed for 2 hours to benzene concentrations of 4.4 and
0.88 mg/ml respectively. In some cases, larvae were treated
during the entire larval life (96 hours) to 4.4 or 8.8
mg/ml. After exposure, the wings of the surviving adults
were mounted and scored for the presence of single and twin
spots. The number of wings scored and the frequency and
size of the spots were recorded. Control experiments were
conducted by exposing larvae of the same age as those in the
treatment groups to the 5% Tween 80 and 5% ethanol vehicle
for the same length of time as the corresponding treated
larvae.
Result: A benzene concentration of 8.8 mg/ml was lethal and 4.4
mg/ml toxic when given for the entire larval life. A
significant increase in the frequency of small single spots
and/or total single spots was seen in the acute and chronic
studies in the excision–repair–defective larvae (mei–9
strain). The acute result was only marginally positive. No
such effect was seen when benzene was given to
excision–repair–proficient larvae (+/mei–9 strain).
Source: BP Chemicals Ltd LONDON
Test substance: Laboratory reagent grade; 99% pure.
13–DEC–1996 (1284)
Appendix D: Benzene SIDS Dossier
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