Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
3. Environmental Fate and Pathways Substance ID: 71–43–2
______________________________________________________________________________
benzene under a headspace of 90% N2–10% CO2 at 35°C. Sterile
controls contained autoclaved sludge and sterile test
chemical. Incubation was conducted for at least 60 days.
Total gas production was measured weekly. Net gas production
(NGP) was expressed as percentage of the theoretical gas
production calculated from the stoichiometry of test
chemical mineralization to methane and carbon dioxide.
Test substance: The test substance was laboratory reagent grade.
Reliability: (2) valid with restrictions
Flag: Risk Assessment
02–SEP–2003 (81)
Type: anaerobic
Inoculum: other
Degradation: = 0 % after 161 day(s)
Result: under test conditions no biodegradation observed
Method: other
GLP: no data
Test substance: no data
Method: The microcosms were amended with NH4–N, PO4–P and one or
more of the following compounds: benzene, toluene, phenol,
catechol, nitrate and/or biocides. Removal of test substance
was determined by GC.
Remark: No standard test procedure, but in accordance with generally
accepted scientific methods and described in sufficient
detail.
Result: Within an incubation time of 63 or 161 days benzene was
neither removed in uncontaminated nor in contaminated
samples to a significant degree regardless if it was present
as sole source substrate or with other metabolizable
compounds.
A second study reported the complete removal of benzene
after 6 months following the in situ anoxic biological
treatment of a hydrocarbon contaminated aquifer under
anaerobic conditions (Battermann G. In: Contaminated Soil.
TNO International Conference. p.711–722. Edited by J.W.
Assink & W.J. Van den Brink. Nijhoff, Dordrecht, The
Netherlands, 1986 (cited in this paper).
In the uncontaminated microcosm, benzene was not degraded
after 56 days but did inhibit the basal rate of
denitrification of the microcosm.
No significant degradation was seen in the contaminated
microcosm after 161 days but the denitrification processes
were unaffected.
Source: Deutsche Shell Chemie GmbH Eschborn;
German Rapporteur
Test condition: Removal of benzene (initial concentration 7–8 mg/l) in
microcosms filled with aquifer core samples (a) from an
uncontaminated site and (b) from the saturated zone below a
jet fuel contaminated interval under denitrifying
conditions.Incubation under nitrogen atmosphere at 12°C.
Test substance: Test substance was reagent grade.
Reliability: (2) valid with restrictions
02–SEP–2003 (544)
Appendix D: Benzene SIDS Dossier
– 211/957 –