Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Species: other: Rana pipiens (Leopard frog)
Endpoint: other
Expos. period: 9 day(s)
Unit: ppm
Method: other: flow–through system
Year: 1982
GLP: no data
Test substance: other TS: reagent grade
Method: Eggs were exposed to 5 resp. 6 different benzene
concentrations within 30 minutes of fertilization. Exposure
was maintained through 4 days after hatching. Average
hatching time was 5 days.
Benzene concentration was measured daily by GLC or HPLC.
Test parameters were egg hatchability, including all embryos
(normal or aberrant) and survival of normal organisms
determined at hatching and 4 days posthatching. Normal
organisms were determined as those organisms that were free
of gross teratic defects.
Result: Benzene conc. Percent Percent survival of normal
[mg/l] hatchability organisms
at hatching 4 days post hatch
0.016 95 95 95
0.048 90 90 90
0.61 81 81 79
2.99 64 64 62
5.07 39 32 32
Log probit analysis was used by the authors to determine the
LC50 at hatching and 4 days after hatching. For Rana pipiens
values of 4.03 resp. 3.66 mg/l were calculated.
Additionally, the authors determined with the same
statistical method LC1– and LC10–values at 4 days
posthatching. For Rana pipiens a LC1–value of 3.2 µg/l and a
LC10–value of 75.6 µg/l was determined.
Source: German Rapporteur
Test condition: Flow–through system:
temperature: 20.2 ± 0.5 °C;
dissolved oxygen: 7.5 mg/l;
water hardness: 96.6 ± 1 mg/l CaCO3;
pH: 7.7 ± 0.02.
Reliability: (2) valid with restrictions
Flag: Risk Assessment
18–MAR–2004 (109)
Appendix D: Benzene SIDS Dossier
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