Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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side continuously perfused with freshwater, the other side
with freshwater containing benzene which was added from a
reservoir by a syringe pump.
20–60 fish were placed in the holding region of the Y–maze
for 15 min before injection of benzene to the water of one
arm was started. After 50–70 min, the number of animals in
each of the two upstream arms of the Y–maze was counted.
Analytical monitoring showed that measured concentrations of
test substance well agreed with calculated concentrations.
The calculated EC50 value represents the concentration of
benzene at which half the expected number of fish would be
found in the test arm with treated water.
Reliability: (2) valid with restrictions
16–MAR–2004 (749)
Type: flow through
Species: Oncorhynchus mykiss (Fish, fresh water)
Exposure period: 96 hour(s)
Unit: mg/l Analytical monitoring: yes
LC50: = 5.3 –
Method: other
Year: 1982
GLP: no data
Test substance: no data
Method: Several parameters were not reported in the study (purity of
test substance, EC0, EC100 values).
Remark: Test procedure in accordance with national standard methods
with acceptable restrictions.
Effect values are related to measured concentrations.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: The method of the APHA. Standard Methods for the Examination
of Water and Wastewater. 14th Edition. American Public
Health Association, New York, 1975 was followed. Groups of
20 juvenile fish (Salmo gairdneri/Oncorhynchus mykiss, mean
weight 13.9 g) were exposed to benzene in freshwater at
13–17 degree C. Dissolved oxygen concentration was about 7
mg/l, pH was 7.9–8, alkalinity 147–165 mg/l as CaCO3 and
water hardness 535–596 mg/l as CaCO3. The fish were not fed
during treatment. The photoperod was 16 h light/ 8 h dark.
Benzene concentrations in the test tanks were monitored
daily by HPLC.
Reliability: (2) valid with restrictions
Flag: Risk Assessment
17–MAR–2004 (287)
Appendix D: Benzene SIDS Dossier
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