Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
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in standard plastic cages.
Reliability: 4 (validity could not be judged)
Original reference not yet available
Result: Benzene caused a dose–dependent increase in the frequencies
of hprt variant (mutant) lymphocytes in both male and female
mice at concentrations of 40 and 100 ppb. At 1000 ppb, the
frequency was also increase when compared with the controls
but this was less than in the 40 and 100 ppb groups.
Females were more sensitive than males.
Source: Deutsche Shell Chemie GmbH Eschborn
06–JAN–1997 (1236)
Type: Somatic mutation assay
Species: Drosophila melanogaster Sex: male/female
Strain: other: see method
Route of admin.: oral feed
Exposure period: up to 96 hours
Doses: 0.88, 4.4, 8.8 mg/ml
Method: other
Year: 1983
GLP: no data
Test substance: other TS
Remark: Reliability: 2 (valid with restriction)
No standard test procedure, but in accordance with generally
accepted scientific standards and described in sufficient
detail
one dose tested per experimental design
The method of Wurgler F.E. et al. Mutation Res. 122,
321–328, 1983 was followed. Benzene was administered to
mei–9 and +/mei–9 strain Drosophila as a solution in 5%
Tween 80 and 5% ethanol. Larvae aged 48 or 72 hours were
exposed for 2 hours to benzene concentrations of 4.4 and
0.88 mg/ml respectively. In some cases, larvae were treated
during the entire larval life (96 hours) to 4.4 or 8.8
mg/ml. After exposure, the wings of the surviving adults
were mounted and scored for the presence of single and twin
spots. The number of wings scored and the frequency and
size of the spots were recorded. Control experiments were
conducted by exposing larvae of the same age as those in the
treatment groups to the 5% Tween 80 and 5% ethanol vehicle
for the same length of time as the corresponding treated
larvae.
Result: A benzene concentration of 8.8 mg/ml was lethal and 4.4
mg/ml toxic when given for the entire larval life. A
significant increase in the frequency of small single spots
and/or total single spots was seen in the acute (larvae aged
72 h) and chronic studies in the excision–repair–defective
larvae (mei–9 strain). The acute result was only marginally
positive. No such effect was seen when benzene was given to
excision–repair–proficient larvae (+/mei–9 strain).
Source: Deutsche Shell Chemie GmbH Eschborn
Test substance: Laboratory reagent grade; 99% pure.
06–JAN–1997 (1284)
Appendix D: Benzene SIDS Dossier
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