Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
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removed and processed for metaphase analysis.
Result: The frequency of chromosome aberrations was increased in the
benzene treated animals, this being significant at 56.3
mg/kg bw/day and above. The male animals were more
sensitive than the females. At doses up to 56.3 mg/kg
bw/day, the abnormalities consisted of chromatid gaps and
single breaks. At 140 mg/kg bw/day, multiple breaks, as
well as gaps and single breaks, occurred and at the higher
doses, cells showing pulverisation of chromosomes were also
seen. Chromosomal damage has also been reported in Swiss
CD–1 mice administered benzene by gavage on two or three
days by Gad–El–Karim M.M. et al. Mutation Res. 135,
225–243, 1984 and Meyne J. & Legator M.S. Envir. Mutagen 2,
43–50, 1980.
Source: BP Chemicals Ltd LONDON
Test substance: "chemically pure" benzene tested.
10–AUG–2000 (1042)
Type: Cytogenetic assay
Species: hamster Sex: male/female
Strain: other
Route of admin.: gavage
Exposure period: 24 hours
Doses: 0, 2.5, 10 ml/kg bw/day (converts to 0, 2.2, 8.8 g/kg bw/day)
Method: other
GLP: no data
Test substance: other TS
Remark: Groups of ten male and ten female Chinese hamsters were
administered benzene in peanut oil. Control animals
received peanut oil. The animals were given two
administrations, of the same benzene dose, at 24–hour
intervals. Animals received an intraperitoneal injection of
colchicine 3 hours after the second dose and were killed 3
hours later. The femurs were removed and processed for
metaphase analysis.
Result: In the males, a dose of 8.8 g/kg bw/day induced a
significant increase in the frequency of chromosome
aberrations, including gaps. In the low–dose group, the
frequency of aberrations did not differ significantly from
the controls. No significant effect was seen in the females
at either dose level.
Source: BP Chemicals Ltd LONDON
Test substance: "Chemically pure" benzene tested.
13–DEC–1996 (1042)
Appendix D: Benzene SIDS Dossier
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