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Appendix D - Dossier (PDF) - Tera

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date: 20–JUL–2005<br />

4. Ecotoxicity Substance ID: 71–43–2<br />

______________________________________________________________________________<br />

Type: static<br />

Species: Salmo trutta (Fish, fresh water, marine)<br />

Exposure period: 1 hour(s)<br />

Unit: mg/l Analytical monitoring: no data<br />

LC50: = 12 –<br />

Method: other<br />

Year: 1974<br />

GLP: no data<br />

Test substance: no data<br />

Source: Deutsche Shell Chemie GmbH Eschborn<br />

German Rapporteur<br />

Test condition: The method employed in this study developed, with certain<br />

modifications, into the standard method to be used in<br />

conditions of consent for effluents, by the Ministry of<br />

Housing and Local Government. Fish Toxicity Tests. Report of<br />

the Technical Committee. HMSO, London, 1969. Groups of ten<br />

yearling fish were exposed to benzene at 10 degree C in<br />

freshwater.<br />

Reliability: (2) valid with restrictions<br />

16–MAR–2004 (1278)<br />

Type: static<br />

Species: Salvelinus malma (Fish, marine)<br />

Exposure period: 96 hour(s)<br />

Unit: mg/l Analytical monitoring: yes<br />

LC50: = 10.5 –<br />

Method: other<br />

Year: 1979<br />

GLP: no data<br />

Test substance: no data<br />

Remark: No EC0, EC100 reported, purity of test substance not<br />

specified.<br />

Result: LD50 confidence limits = 7.9–13 mg/l.<br />

Most deaths occurred during the first 12 h of exposure.<br />

Smolts acclimated to saltwater (salinity 28–30 g/l) were<br />

more sensitive to benzene (LC50 value 5.5 mg/l, 95%<br />

confidence limits 4–7 mg/l) than smolts in freshwater (LC50<br />

value 10.5 mg/l, confidence limits 7.8–13.2 mg/l).<br />

Effect values are related to initial nominal concentrations.<br />

Source: Deutsche Shell Chemie GmbH Eschborn<br />

German Rapporteur<br />

Test condition: Dolly varden were exposed to benzene (12 fish/concentration)<br />

in freshwater at 8 degree C. Solutions were aerated during<br />

the final 48 h to ensure dissolved oxygen content at >85% of<br />

the saturation level.<br />

Concentrations of benzene as monitored by<br />

UV–spectrophotometry (at 254 nm) decreased to ca. 75% of<br />

initial value in 24 h and to ca. 10% after 96h,<br />

respectively.<br />

Test substance: Purity not specified<br />

Reliability: (2) valid with restrictions<br />

Flag: Risk Assessment<br />

17–MAR–2004 (787)<br />

<strong>Appendix</strong> D: Benzene SIDS <strong>Dossier</strong><br />

– 254/957 –

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