Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Survival, behaviour and reproduction were observed at the time
of daily renewal.
Concentration of benzene was measured by HPLC.
Test substance: purity 99.9 %
Reliability: (1) valid without restriction
Flag: Risk Assessment
18–MAR–2004 (825)
Species: other aquatic crustacea: Callinectes sapidus
Endpoint: mortality
Unit: mg/l Analytical monitoring: yes
EC : 1 –
Method: other
Year: 1981
GLP: no data
Test substance: no data
Result: A significantly greater number of benzene treated animals
died before molting (55.8% vs. 23%).
The mean time required to molt was significantly increased
for animals exposed to benzene (control: 33 days, treated:
50 days).
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Juvenile C. sapidus (blue crab, carapace widths 1–8 cm),
collected from the wild, were kept in 750 ml artificial sea
water (osmolality 280–380 osmol, temperature 23 degree C,
12h:12h dark/light cycle) for at least 2 weeks before the
experiment was started. Crabs were induced to autotomize the
3rd appendage. One group was exposed to 1 mg/l benzene
(nominal concentration, see below) (added as stock solution
in ethanol, water with benzene was changed daily), the other
received ethanol only (water was changed twice a week).
To assess the concentration of benzene in the water, the
actual concentrations were monitored in a control experiment
by GC analysis. Concentrations fell linearly within 7 h to
about 20% of initial level. It was concluded that the
animals were exposed to 0.26–0.3 mg/l benzene for 18 h a
day.
Reliability: (3) invalid
Flag: Risk Assessment
17–MAR–2004 (173)
Appendix D: Benzene SIDS Dossier
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