Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
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In 1991 and 1992, when the benzene concentrations were
brought down, there was a concomitant decrease in the
frequencies of chromosomal aberrations.
Source: Deutsche Shell Chemie GmbH Eschborn
06–JAN–1997 (1145)
Type: Cytogenetic assay
Species: mouse Sex: male/female
Strain: other: Swiss Lane Petter
Route of admin.: gavage
Exposure period: 24 hours
Doses: 0, 10.2, 25.6, 64, 160, 400, 2500 ul/kg bw/day (converts to 0,
8.96, 22.5, 56.3, 140, 352, 2198 mg/kg bw/day)
Method: other: in compliance with OECD Guide–line 475
Year: 1984
GLP: no data
Test substance: other TS
Remark: Benzene was administered in peanut oil. Groups of ten male
and ten female Swiss Lane Petter mice were exposed to each
benzene dose. Control animals received peanut oil. The
animals were given two administrations, of the same benzene
dose, at 24–hour intervals. Animals received an
intraperitoneal injection of colchicine 3 hours after the
second dose and were killed 3 hours later. The femurs were
removed and processed for metaphase analysis.
Reliability: 2 (valid with restriction)
Comparable to guideline study with acceptable restrictions
no positive control, one sampling time
Result: The frequency of chromosome aberrations was increased in the
benzene treated animals, this being significant at 56.3
mg/kg bw/day and above in males and females. The male
animals were more sensitive than the females. At doses up
to 56.3 mg/kg bw/day, the abnormalities consisted of
chromatid gaps and single breaks. At 140 mg/kg bw/day,
multiple breaks, as well as gaps and single breaks,
occurred and at the higher doses, cells showing
pulverisation of chromosomes were also seen. Chromosomal
damage has also been reported in Swiss CD–1 mice
administered benzene by gavage on two or three days by
Gad–El–Karim M.M. et al. Mutation Res. 135, 225–243, 1984
and Meyne J. & Legator M.S. Envir. Mutagen 2, 43–50, 1980.
Source: Deutsche Shell Chemie GmbH Eschborn
Test substance: "chemically pure" benzene tested.
06–JAN–1997 (1042)
Appendix D: Benzene SIDS Dossier
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