Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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Test substance: >= 80% pure.
Reliability: (4) not assignable
17–MAR–2004 (673)
Species: Daphnia magna (Crustacea)
Exposure period: 48 hour(s)
Unit: mg/l Analytical monitoring: no
EC50: 31.2 –
Method: other
Year: 1983
GLP: no data
Test substance: other TS
Remark: 95% confidence interval: 22.2–43.3 mg/l.
In the study, values are presented as mmol/m3.
Significant methodological deficiencies
No analytical monitoring, applied concentrations of test
substance calculated from solubility data.
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Daphnia (4–6 days old) were exposed to benzene in distilled
water as follows:
10 animals in 24 ml glass vials with Teflon–lined screw caps
completely filled,
5 concentrations of benzene and control,
temperature: 21–25 degree C,
pH ca. 7 at start and ca. 6 at end of exposure
dissolved oxygen ca. 9 mg/l at start and 5 mg/l at end of
exposure.
Benzene was added as saturated stock solution in water
(lower phase of water/benzene mixture in a separatory
funnel). The concentration of the stock solution was
calculated from solubility data and it was assumed that loss
of benzene due to volatilisation was neglible.
No analytical monitoring was performed.
Test substance: Purity: at least 97% (no further specification)
Reliability: (3) invalid
17–MAR–2004 (121)
Appendix D: Benzene SIDS Dossier
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