Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
4. Ecotoxicity Substance ID: 71–43–2
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17–MAR–2004 (895)
Type: static
Species: Morone saxatilis (Fish, estuary, marine)
Exposure period: 24 hour(s)
Unit: mg/l Analytical monitoring: no data
LC50: = 6.1 –
Method: other
GLP: no data
Test substance: other TS
Remark: Similar exposure for 96 hours gave an LC50 value of 5.8 ppm
(converts to 5.1 mg/l).
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Juveniles (mean weight: 6.0 g) were exposed at 16 degree C
in saltwater (salinity: 25 g/l).
Benzene concentration was assayed by gas chromatography at
0, 24, 48, 72, and 96 hours.
Test substance: >99% pure.
Reliability: (2) valid with restrictions
16–MAR–2004 (94)
Type: static
Species: Mugil curema (Fish, estuary, marine)
Exposure period: 48 hour(s)
Unit: mg/l Analytical monitoring: no
LC50: = 22 –
Method: other
GLP: no data
Test substance: no data
Remark: Significant methodological deficiencies
No analytical monitoring, no EC0 value, no confidence limits
reported, no. of animals not reported. According to the
authors, benzene solutions were "emulsified".
Source: Deutsche Shell Chemie GmbH Eschborn
German Rapporteur
Test condition: Juvenile white mullet (0.5–1.0 g) were captured from the
wild and kept at 18–22 degree C in filtered, well–aerated
seawater (salinity: 35 g/l) for 10 days prior to the assay.
Fish were fed ad libitum.
48 h LC50 bioassays were conducted in 2.5 l glass bottles
with water aerated before benzene was added from stock
solution (1000 mg/l).
Reliability: (3) invalid
16–MAR–2004 (245)
Appendix D: Benzene SIDS Dossier
– 248/957 –