Appendix D - Dossier (PDF) - Tera

date: 20–JUL–2005
5. Toxicity Substance ID: 71–43–2
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Species: mouse Sex: female
Strain: Swiss Webster
Route of administration: inhalation
Exposure period: gestation days 6–15
Frequency of treatment: daily/6 hours per day
Duration of test: 12–week–old offspring
Doses: 10 ppm/day (converts to 0.033 mg/l/day); 5–10
mice/dose
Control Group: yes, concurrent no treatment
Method: other
GLP: no data
Test substance: no data
Remark: Control group consisted of five to ten females exposed to
filtered conditioned air. Exposed, pregnant mice were
allowed to proceed through normal parturition. At 10 weeks
of age, the offspring [numbers unspecified] were re–exposed
for 2 weeks to 0.033 mg/l. After the 2 weeks, the animals
were sacrificed and their spleens and femurs removed for
enumeration of haematopoietic colony forming cells. Control
offspring exposed in utero to air were similarly exposed at
10 weeks of age to 0.033 mg/l benzene for 2 weeks.
Result: The in utero benzene–exposed offspring showed a marked
reduction in the numbers of bone marrow CFU–E on
re–exposure. No such effect was seen in the in utero
air–exposed animals. Both groups of offspring had decreased
splenic granulocyte/macrophage colony forming unit–culture
numbers on re–exposure but this was more severe in the in
utero benzene–exposed offspring.
Source: BP Chemicals Ltd LONDON
25–OCT–2000 (607)
Species: mouse Sex: female
Strain: other
Route of administration: inhalation
Exposure period: gestation days 5–14
Frequency of treatment: daily/three 4–hour exposures per day
Duration of test: gestation day 17
Doses: 0.5, 1.0 mg/l; 15 mice/dose
Control Group: yes, concurrent no treatment
Method: other
GLP: no data
Test substance: no data
Remark: CFLP (LATI–Gödöllö, Hungary) strain mice were used. Control
group consisted of 115 females exposed to air. Dams were
killed on day 17 and foetuses examined as described by Hudak
A. & Ungvary G. Toxicology 11, 55–63, 1978.
Result: No effects were seen on the numbers of live or dead or
resorbed foetuses or on the incidence of minor anomalies or
total malformations. The incidences of weight and skeletal
retarded foetuses were increased, this being statistically
significant.
Source: BP Chemicals Ltd LONDON
24–OCT–2000 (1188)
Appendix D: Benzene SIDS Dossier
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