Environmental Profiles of Chemical Flame-Retardant Alternatives for

Species, strain: Mouse lymphoma L5178Y
Metabolic activation: None, phenobarbital-induced or PCB-induced
Concentrations: 0, 0.005-0.03 :L/mL for phenobarbital-induced (4 concentrations), and 6
concentrations up to 0.070 :L/mL for non-induced or PCB-induced mouse
Purity: Not reported
Method: Ten cells per concentration were analyzed.
Results: TDCPP increased the incidence of sister chromatid exchanges in mouse lymphocytes
under all three test conditions.
Reference: Brusick et al., 1979; also Litton Bionetics, Inc., 1977
Additional Information
One submitted confidential study reported negative results in a sister chromatid exchange assay.
The data for this study are not adequate because the cell line was not identified. Fyrol FR-2 did
not induce sister chromatid exchanges when applied to 3- to 4-day-old chicken embryos (Bloom,
1984).
Chromosomal Aberration in Vivo:
C Mammalian Bone Marrow Chromosomal Aberration Test (OPPTS Harmonized
Guideline 870.5385)
The available study provides sufficient evidence that TDCPP did not induce chromosomal
aberrations in mice exposed at the maximum tolerated dose of 760 mg/kg.
Type: Bone marrow chromosomal aberration in vivo
Species, strain: Mouse, CD-1, 4-8 males/group
Metabolic activation: None
Concentrations: 0, 0.05, 0.17, and 0.5 mL/kg; using the specific gravity of 1.52, the doses were
0, 76, 260, or 760 mg/kg. The highest dose was the maximum tolerated dose. Negative control
was DMSO
Exposure duration, frequency: By oral gavage in once or daily on 5 consecutive days.
Purity: Technical grade; Not reported
Method: Mice were sacrificed at 6, 24, and 48 hours after single dose or 6 hours after the last of
5 doses. Between 233 and 400 cells were scored, rather than 500/animal. Triethylenemelamine
was positive control.
Results: No evidence of increased frequency of chromosomal aberrations with TDCPP. TBPP
was also negative at doses up to 1,000 mg/kg. Positive control produce expected large increase
in micronucleated polychromatic erythrocytes.
Reference: Brusick et al., 1979; Litton Bionetics, Inc., 1978
C Mammalian erythrocyte micronucleus test (OPPTS Harmonized Guideline
870.5395)
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