Environmental Profiles of Chemical Flame-Retardant Alternatives for

Acute Toxicity to Freshwater Invertebrates (OPPTS Harmonized Guideline 850.1010;
OECD 202)
Conclusion:
The acute toxicity data for freshwater invertebrates were judged inadequate to meet the endpoint.
However, if the results of the study cited by Ref. 29 (see below) are confirmed independently,
the endpoint might be satisfied given the high degree of agreement of the two available studies in
freshwater invertebrates.
Basis for Conclusion:
The available data are summarized in Table 4-2. A flow-through study revealed a 48-hour LC 50
of Proprietary A with Daphnia magna of 3.8 mg/L (95% CI: 3.5-4.2 mg/L) and a NOEC of 1.6
mg/L (Unpublished study conducted in 1999, summarized in Ref. 4, 5). Although some of the
conditions of the study design (such as number of organisms, and water temperature and
chemistry) appear to meet OPPTS Harmonized Guideline 850.1010, other aspects of the study,
including compound purity and condition and fertility of the organisms in culture, were not
reported in the summary. The amount of solvent used in the control group and the Proprietary A
treatments might have exceeded the recommended maximum solvent concentration, as per the
OPPTS Guideline (100 mg/L), but this does not appear to have affected the study results. A 48hour
LC 50 of 4.6 mg/L and a NOEC of 1.8 mg/L were reported for daphnia in a study by Ref. 43,
as cited in Ref. 29. Although the results of the study by Ref. 43 are in agreement with those of
Ref. 4, 5, the study by Ref. 43, or a study summary, was not available to allow for an
independent evaluation of these data. Confirmation of the results of the study by Ref. 43 might
allow the acute freshwater invertebrate toxicity endpoint to be satisfied.
Acute Toxicity to Marine/Estuarine Invertebrates (OPPTS Harmonized Guideline
850.1035)
Conclusion:
No available acute marine/estuarine invertebrate toxicity data.
Basis for Conclusion:
No acute toxicity studies in marine/estuarine invertebrate species were located.
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