Environmental Profiles of Chemical Flame-Retardant Alternatives for

Chromosomal Aberration in Vivo:
C Mammalian Bone Marrow Chromosomal Aberration Test (OPPTS Harmonized
Guideline 870.5385)
The available study provides sufficient evidence that Proprietary A did not induce chromosomal
aberrations in mice exposed at the maximum tolerated dose of 760 mg/kg.
Type: Bone marrow chromosomal aberration in vivo
Species, strain: Mouse, CD-1, 4-8 males/group
Metabolic activation: None
Concentrations: 0, 0.05, 0.17, and 0.5 mL/kg; using the specific gravity of 1.52, the doses were
0, 76, 260, or 760 mg/kg. The highest dose was the maximum tolerated dose. Negative control
was DMSO
Exposure duration, frequency: By oral gavage in once or daily on 5 consecutive days.
Purity: Technical grade; Not reported
Method: Mice were sacrificed at 6, 24, and 48 hours after single dose or 6 hours after the last of
5 doses. Between 233 and 400 cells were scored, rather than 500/animal. Triethylenemelamine
was positive control.
Results: No evidence of increased frequency of chromosomal aberrations with Proprietary A.
[Chemical 2] was also negative at doses up to 1,000 mg/kg. Positive control produce expected
large increase in micronucleated polychromatic erythrocytes.
Reference: Ref. 12; Ref. 34
C Mammalian erythrocyte micronucleus test (OPPTS Harmonized Guideline
870.5395)
Proprietary A administered as 2,000 mg/kg by an unspecified route to mice did not induce
micronuclei in bone marrow erythrocytes (Ref. 60 as reported in Ref. 61).
DNA Damage and Repair:
C Unscheduled DNA synthesis in mammalian cells in culture (OPPTS Harmonized
Guideline 870.5550)
Type: Unscheduled DNA synthesis in mammalian cells (hepatocytes) in culture
Species, strain: Rat, Wistar, male
Metabolic activation: With or without phenobarbital-induction
Concentrations: 0, 0.05, and 0.1 mM
Purity: Not reported
Vehicle: DMSO
Method: Cultured hepatocytes exposed to Proprietary A or [Chemical 2] for 18-19 hours.
Incorporation of radiothymidine into DNA.
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