Environmental Profiles of Chemical Flame-Retardant Alternatives for

TDCPP administered as 2,000 mg/kg by an unspecified route to mice did not induce micronuclei
in bone marrow erythrocytes (Thomas and Collier, 1985 as reported in WHO, 1998).
DNA Damage and Repair
C
Unscheduled DNA synthesis in mammalian cells in culture (OPPTS Harmonized
Guideline 870.5550)
Type: Unscheduled DNA synthesis in mammalian cells (hepatocytes) in culture
Species, strain: Rat, Wistar, male
Metabolic activation: With or without phenobarbital-induction
Concentrations: 0, 0.05, and 0.1 mM
Purity: Not reported
Vehicle: DMSO
Method: Cultured hepatocytes exposed to TDCPP or TBPP for 18-19 hours. Incorporation of
radiothymidine into DNA.
Results: TDCPP was not genotoxic at 0.05 mM, but at 0.1 mM, a moderate response was
observed in hepatocytes from untreated rats, but not phenobarbital-treated rats. TBPP, the
positive control, yielded positive results in induced and non-induced hepatocytes.
Reference: Soederlund et al., 1985
Additional Information
A confidential study reported negative results for TDCPP and its metabolites (not identified) in
cultured primary hepatocytes from male Sprague-Dawley rats.
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