The Influence Of Priming Two Cucumber Cultivar Seeds

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A J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 112-121, 2010 shoots/ culture and 3.34 leaves/ culture respectively (Table 2, Fig. 3. b-d), however the highest length of shoot was obtained at 7.5 mgl -1 Kin which was 2.89 cm. Whereas no embryo and shoot per culture was obtained in MS medium free-hormone and the lowest length of shoot was obtained in MS medium containing 10 mgl -1 BA which was 1.50 cm. Results of other researchers showed that BA is more effective than Kin for multiple shoot induction like nodal culture of lagerstroemia flos-regina (Paily and D , Souza, 1986). In general, BA has been frequently reported to induce better shoot 111 multiplication than other cytokinins, particularly in tree species. Its effectiveness has been demonstrated in juvenile as well as mature tissues of Calophyllum inophyllum, Euggenia grandis and Fragraea fragns (Rao and Lee, 1982). Ahmad (1989) observed the highest length of shoots from Acacia mangium (0.95 cm) on MS medium with 1 mgl -1 Kin from in vitro nodal explant. Faisal et al. (2006) reported the establishment of shoot in vitro from a nodal segment in 10 mgl -1 cytokinin, while Faisal et al (2007) got the best results from shoot segment at 2.5 mgl -1 Kin. Fig. (3): a: somatic embryos and shoots developed on 2.5 mgl -1 BA; b: somatic embryos and shoots developed on 7.5 mgl -1 Kin; c: shoot induction from somatic embryos explants on 2.5 mgl -1 BA; d: shoot induction from somatic embryos explants on 7.5 mgl -1 Kin. Table (2): Effects of different concentrations of BA and Kin on the growth response of Codiaeum variegatum leaf explants after 8 weeks of culture. Growth regulator mg l -1 No. of somatic embryos induced from callus No. of shoots/ culture No. of Leaves/ culture average Length of Shoots control 0.0 1.00e 0e 0c 0d Kin BA B 2.5 2.01de 1.85d 3.30a 1.63c 5.0 3.55c 2.33cd 2.81ab 1.83bc 7.5 3.70c 3.45b 3.35a 2.89a 10 2.05de 2.50cd 2.83ab 1.93a 2.5 7.95a 4.58a 3.13a 2.33ab 5.0 7.71ab 3.20bc 2.10b 2.06bc 7.5 6.69b 2.25d 2.15b 1.50c 10 2.88cd 2.75bcd 2.50ab 1.50 c * Means followed by the same letter within each character (column) do not differ significantly (P≤0.05) according to Duncan's Multiple Range Test (Duncan ,1955). D (cm)
J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 112-121, 2010 Effect of different concentrations of BA+ Kin on in vitro shoot multiplication: Different concentrations of BA ranging from 2.5 mgl -1 to 5 mgl -1 with Kin ranging from 2.5 mgl -1 to 5 mgl -1 in MS medium were also used for in vitro shoot multiplication. It was observed that when 3 mgl -1 BA +4.5 mgl -1 Kin was used in MS medium, average of 4.53 shoots / culture were formed. When the concentration of BA was increased from 2.5 mgl -1 to 3 mgl -1 BA with 5 mgl -1 to 4.5 mgl -1 Kin the number of shoots pre culture formed was also increased. At this concentration, maximum number of leaves per culture and the maximum length of shoot was observed in the same medium which were 4.7 leaves/ culture and 3.7 cm respectively (Table 3, Fig. 4 a-b). Further increase in concentration of A BA, average number of shoots per culture was decreased. At 3.5 mgl -1 BA with 4 mgl -1 Kin, 4.20 shoot/ culture and 4 leaves/ culture were obtained. At MS medium free-hormone minimum number of shoots per culture and number of leaves per culture was formed (2.80 shoot/ culture and 3.2 leaves/ culture). Ahmad (1989) also reported the best shoot response of Acacia margium on MS medium supplemented with BA+ Kin. However, Shabbir et al (2009) reported best shoot induction response in MS medium containing (1.5 mgl -1 BA+ 0.5 mgl -1 Kin) and Hameed et al (2006) and Arshed et al (2005) used MS medium supplemented with combinations of BA and Kin for shoot induction from nodal meristem and nodal segments respectively. Fig. (4): In vitro regeneration of Codiaeum variegatum. A-B: Multiple shoot regeneration from callus on MS medium containing 3.0 mgl -1 BA + 4.5 mgl -1 Kin. Table (3): Effects of different concentrations of BA + Kin on the growth response of Codiaeum variegatum leaf explants after 8 weeks of culture. Growth regulator mg l -1 No. of shoots BA + Kin developed B No. of Leaves per culture average Length of Shoots 0.0 2.80abc 3.2a 1.48d 2.5 + 5.0 3.92ab 3.3a 2.5bc 3.0 +4.5 4.53a 4.7a 3.7a 3.5 + 4.0 4.20a 4.0a 3.5ab 4.0 +3.5 3.85abc 3.8a 2.7abc 4.5 + 3.0 2.33bc 3.6a 1.9cd 5.0 + 2.5 2.00c 3.3a 1.5d * Means followed by the same letter within each character (column) do not differ significantly (P≤0.05) according to Duncan's Multiple Range Test (Duncan ,1955). (cm) 111
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The Influence Of Priming Two Cucumber Cultivar Seeds

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