The Influence Of Priming Two Cucumber Cultivar Seeds

J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 153-161, 2010
from local breed of black goats from 35 flocks of
different localities (districts and sub districts)
which include Zakho, Batel, Sumaill, Aqra and
Center of Duhok province.
2. Blood Smears
Method of preparation of blood smears and
Giemsa staining was used as described by Adam
et al., (1971). The thin and thick blood smears
were prepared from the peripheral ear vein.
Before the sample, the site was punctured,
clipped and wiped with 70% ethanol and
allowed to dry the first drop of blood always
discarded, a drop of blood was taken on a clean
glass microscope slide, spread by another slide
at an acute angle; air dried, and fixed by
methanol 5 minute and stained with 10% Giemsa
stain. 30 minutes
3. Serologic Test for Detection of A. ovis
The anaplasmosis competitive enzyme-linked
immunosorbent assay (cELISA) was performed
with serum sample using the Anaplasma
Antibody Test Kit, cELISA from VMRD
Inc.(2006) (Pullman, WA, USA) (Knowles et
al., 1996) following the manufactures’
instructions. This assay detects serum antibodies
to a major surface protein (MSP5) of A.
marginale, A. centrale, A. ovis and A.
phagocytophilum (Dreher et al., 2005).
4. Hematological Examination:
Ten milliliters of blood was drained from
jugular vein puncture, after the area was
prepared aseptically, by clipping and then
154
soaking with 70% ethyl alcohol. Three milliliters
of whole blood were mixed in disposable clean
plastic tube with an anticoagulant (EDTA) and
used for the estimation hematological
parameters, while remaining seven milliliters of
the blood were deposited in tube free from
anticoagulant, to obtain serum for estimation of
serological test by ordinary centrifuge 5000 rpm
spanned for 5 minutes and separated serum kept
at – 20°C until used. Hematological parameters
included, total erythrocyte count (RBC), packed
cell volume (PCV), hemoglobin concentration
(Hb), differential leukocyte count, mean
corpuscular volume (MCV), mean corpuscular
hemoglobin (MCH), and mean corpuscular
hemoglobin concentration (MCHC), were done
by methods of Coles (1986).
Statistical analysis:
The results were analyzed by chi- square test
(X 2 ) (Tekin, 2003).
RESULTS
Depending on morphological characteristics
of the Anaplasma inclusion body in erythrocyte
by Giemsa stained blood smear, the Anaplasma
ovis was identified by light microscopic examination
of Giemsa stained blood smear. A. ovis
appeared uniform dark dote like circular
body periphery to erythrocyte as single, double and
triple inclusion bodies as shown in (Fig.1).
The Anaplasma parasitemia was ranged from (2.8-
12.2 %) with the mean value (7.16 ± 2.98).
A B
Fig (1): Anaplasma ovis in Giemsa staining blood smear (X1000)