The Influence Of Priming Two Cucumber Cultivar Seeds

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J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 112-121, 2010 Effects of different concentrations of NAA, IAA and IBA alone with (¼ and ½ strength) MS medium on in vitro rooting: Two concentrations of auxin (0.5 and 1 mgl -1 ) of NAA, IAA and IBA at two levels of MS medium (¼ and ½ strength) were used for in vitro roots formation. The best response was observed when 0.5 mgl -1 IBA was added to ¼ strength MS medium which was (70%) while 60% of the shoots were rooted when 0.5 mgl -1 IBA was added to ½ strength MS medium. However the shoots were not obtained when shoots were cultured in (¼ and ½ strength) MS free-hormone (Table 4). The lowest root induction response (20%) was observed on ½ strength MS medium supplemented with 0.5 mgl -1 NAA. A large number of roots, root length and root percentage (2.67 roots /explant, 3.56cm and 70%) respectively, was observed when 0.5 mgl -1 IBA was used in ¼ MS medium. While a large number of roots, root length (2.55 roots /explant and 3.34 cm) respectively, was Growth 111 observed when 0.5 mgl -1 NAA and 1 mgl -1 NAA were used in ½ strength MS medium and 60% root percentage was observed when 0.5 mgl -1 IBA was added to ½ strength MS medium . In the same table, minimum number of roots per shoot and root length were formed (1.23 roots/ shoot and 1.71cm) when 1 mgl -1 NAA and 0.5 mgl -1 NAA were added to ¼ strength MS medium respectively. Also minimum number of roots per shoot and root length were formed (1.34 roots/ shoot and 2.09 cm) when 0.5 mgl -1 NAA and 1mgl -1 IBA were added to ¼ strength MS medium respectively. IBA has been reported to have stimulatory effect on root induction in many plant species including Alnus glutinosa (Perinet and Lalonde, 1987), Morus indica (Chand et al., 1995), Balanites aegyptica (Mansor et al., 2003) and Bambusa vulgaris (Ndiaye et al., 2006). Table (4): Effects of different concentration of NAA, IAA and IBA and salt strength of MS medium on shoot rooting of Codiaeum variegatum after 4 weeks of culture. regulator mg l -1 Media con. % shoots showing root * Means followed by the same letter within each character (column) do not differ significantly (P≤0.05) according to Duncan's Multiple Range Test (Duncan ,1955). The rooted plantlets of Codiaeum variegatum were carefully removed from rooting medium and washed carefully, then transferred to greenhouse in small plastic pots with medium consisting of (sand and peat moss, 1:1). The plants were finally hardened by gradually reducing the humidity. After four weeks from transplanting, the survival percentage reached to 55%. ¼ MS ½ MS Number of roots/shoot Root length (cm) % shoots showing root Number of roots/shoot control 0.0 0a 0c 0f 0a 0c 0f NAA IAA IBA Root length 0.5 50a 1.42b 1.71e 20ab 1.34b 3.34ab 1 50a 1.23b 2.23de 30ab 2.55a 2.19de 0.5 60a 2.08a 2.47cd 50a 2.29a 2.58cd 1 50a 2.38a 2.43cd 50a 2.33a 2.94bc 0.5 70a 2.67a 3.56a 60a 2.47a 2.45cd 1 60a 2.42a 3.24ab 50a 1.83ab 2.09de (cm) REERENCE - Ahmad, D. H. (1989). Micropropagation of Acasia margium from aseptically germinated seedlings. Journal of Tropical Forest Science 3(3):204-208. - Ammirato, P. V. (1983). Embryogenesis. In: Handbook of Plant Cell Culture Vol. 1 Evan DA,sharp WR, Ammirato and Yamada (Eds.), MacMillan Publishing Co., New York, pp. 82-123.
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The Influence Of Priming Two Cucumber Cultivar Seeds

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