The Influence Of Priming Two Cucumber Cultivar Seeds

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J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 122-130, 2010 239 Fig. (13): Effect of MS Salt strength and NAA on root number / shoot of eggplant. Fig. (14): Effect of MS Salt strength and NAA on root length / shoot of eggplant . Fig. (15): Effect of MS Salt strength and NAA on roots number / shoot of tomato.
J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 122-130, 2010 Fig. (16): Effect of MS Salt strength and NAA on root length / shoot of tomato. The use of cytokinins and auxins at different concentrations are commonly used in in vito propagation of plants, exogenous application of BA in the prescence of auxin plays an important role in culture initiation and multiplication stages. BA induces cell division and has important role in growth of lateral buds by releasing them from apical dominance, auxin promotes cell elongation and division and stimulates vascular differentiation in plant tissues ( Davies, 1995) . Differences were noticed in response and to totipotency of the explants of eggplant and tomato due to the exogenous application of BA and auxin to culture medium during the initiation stage , this may attributed to the levels of endogenous hormones in both species under study. The frequency of initiation was higher in tomato explants than eggplant explants in hormone free MS media , this most likely due to the high levels of endogenous hormones in tomato since high rates of explant initiation and growth rate of tomato shoots were observed in hormones free MS medium. A high number of Solanaceae family species were propagated in vitro by using different explants and propagation methods (Magioli et al,1998, Sharma and Rajam , 1995, Ma et al .1999 , Franklin et al . 2004 and Iwamoto and Eura , 2006). The elongated shoots were transferred to rooting medium for root induction. Roots were initiated in eggplant shoot after two weeks of culture. In tomato, shoots roots at lowers surface of shoots were induced during the subculture at initiation and multiplication stages. Among the treatments tested, shoots of eggplant and tomato cultured on a 1/2 strength MS medium supplemented with 0.3 mgl -1 NAA produced the highest average root number and length per shoot (Fig 13,14 15,16) . In vitro rooted plantlets were acclimatized carefully and planted in pots containing steril peat moss. Within these regenerated plants ,95% of plantlets was survived uder natural condition. REFERENCES - Davies, P. 1995 . The plant hormones ,their nature ,occurrence and functions , In .P. J Davies (Id) Plant hormones. Physiology and Biochemistry and Molecular Edioloag . kluwer Academic Publishers Dorderecht , 1995 ,pp 1-12 . - Duncan, D. B (1955). Multiple range and multiple F.teses. Boim., 11:1-42. - Franklin ,G. , C.J . Sheeba and L. Sity , 2004 . Regeneration of eggplant (Solanum melongena L.) from root explants In vitro Cell, Dev. Biol, Plant 40: 188- 191. - Iwamoto Y. and H. Eura 2006. Efficient plant regeneration from eggplant rootstock culture and its wild relatives . Plant Biotechnology 23: 525-529. - Ma.Y.K., Kato K. and M. Masuda. 1999. Efficient callus induction and shoot regeneration by anther culture in male sterile of tomato (Lcopersicon esculentum) J.Japanee. Soc. Hort. Sci , 68:768-773. - Magioli C. Rocha, A.P.M., de Oliveira, D. E and Mansur. E (1998) Efficient shoot organogenesis of egg plant( Solanum melongena (L.) induced by thidiazuron . Plant Cell Rep. 17:661-663. - Murashige T. and Skoog F. 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant. 15:473 -497. - Sharma, P. and M.V. Rajam 1995. Genotype explant and position effects on organogenesis and embryogenesis in eggplant ( Solanum melongena L.) J. Exp. Bot. 46 : 135-141. - Smith. R.H. 2000. Plant Tissue Culture Techniques and Experiments, 2nd. edition , A cademic press. - Trigiano, R. N. and D. J. Gray. 2000. Plant Tissue Culture Concepts and Laboratory Exercies. 2 nd ed. U. S. A., Boca Raton New York. Washington DC. 23:
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The Influence Of Priming Two Cucumber Cultivar Seeds

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