The Influence Of Priming Two Cucumber Cultivar Seeds

J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 122-130, 2010
Fig. (16): Effect of MS Salt strength and NAA on root length / shoot of tomato.
The use of cytokinins and auxins at different
concentrations are commonly used in in vito
propagation of plants, exogenous application of
BA in the prescence of auxin plays an
important role in culture initiation and
multiplication stages. BA induces cell division
and has important role in growth of lateral buds
by releasing them from apical dominance, auxin
promotes cell elongation and division and
stimulates vascular differentiation in plant
tissues ( Davies, 1995) . Differences were
noticed in response and to totipotency of the
explants of eggplant and tomato due to the
exogenous application of BA and auxin to
culture medium during the initiation stage , this
may attributed to the levels of endogenous
hormones in both species under study. The
frequency of initiation was higher in tomato
explants than eggplant explants in hormone free
MS media , this most likely due to the high
levels of endogenous hormones in tomato since
high rates of explant initiation and growth rate of
tomato shoots were observed in hormones free
MS medium. A high number of Solanaceae
family species were propagated in vitro by using
different explants and propagation methods
(Magioli et al,1998, Sharma and Rajam , 1995,
Ma et al .1999 , Franklin et al . 2004 and
Iwamoto and Eura , 2006). The elongated shoots
were transferred to rooting medium for root
induction. Roots were initiated in eggplant shoot
after two weeks of culture. In tomato, shoots
roots at lowers surface of shoots were induced
during the subculture at initiation and
multiplication stages. Among the treatments
tested, shoots of eggplant and tomato cultured on
a 1/2 strength MS medium supplemented with
0.3 mgl -1 NAA produced the highest average
root number and length per shoot (Fig 13,14
15,16) . In vitro rooted plantlets were
acclimatized carefully and planted in pots
containing steril peat moss. Within these
regenerated plants ,95% of plantlets was
survived uder natural condition.
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