The Influence Of Priming Two Cucumber Cultivar Seeds

J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 148-152, 2010
841
OVARIAN RESPONSE IN SUPEROVULATED KARADI
YERLING EWES TREATED WITH INSULIN *
ARAZ G. PEDAWY and JALAL E. ALKASS
Dept. of Animal Production, College of Agriculture. University of Duhok, Kurdistan Region-Iraq
(Received: September 23, 2010; Accepted for publication: December 8, 2010)
ABSTRACT
Twenty five Karadi yearling ewes and 52 kg in weight were divided randomly into two groups;Group one (control,
n= 13) and Group two (insulin treatment, n=12). Estrus was induced first by intravaginal sponges and PMSG .
Following estrus were pre-treated with HCG and estradiol and animals in both groups were superovulated using 20
IU FSH i.m. in six divided descending doses .i.e. 4/4 and 3/3 and 3/3 at 12 h interval for three consecutive days .Prior
to gonadotropins adminstarion, Animals in group one were given a normal saline and group two animals were given
long acting purified bovine insulin 0.2 IU /kg body weight per day s.c. for three consecutive days started on day 7 of
the estrus cycle. After PGF2α treated, two rams fitted with marking crayons were introduced with ewes for detection
of estrus, and then bred with natural service at approximately 12 hrs interval until the end of estrus. Three days
following mating, all animals were sacrificed to determine ovulation rate. Results revealed that percent ewes
responded to treatment were 69.23% for control group and 75.00% for insulin treated group. Ovulation rate
averaged 1.44±0.22 and 1.56±0.24 for the control and insulin groups, respectively, and the unovulated large follicles
/animal averaged 0.50±0.19 and 0.84±0.19, for control and insulin group, respectively.Percent ova recovered was 84.6
and 35.7% for control and treated groups, respectively.
KEY WORDS: Insulin, Ovulation , Karadi, Ewes.
A
INTRODUCTION
pplication of insulin to modulate
reproductive functions in livestock is a
fairly recent development (Selvaraju et al.,
2003). Moreover , in vitro, studies indicated that
insulin and IGF-1 are important mediators of
follicle development , steriodogenesis , oocyte
maturation and subsequent embryo development
(Gong et al.,1993 a; Gong et al ,1994;Totey et al
.,1996;Gong et al .,1993b). Administration of
insulin increases intrafollicular and peripheral
IGF-1 levels in cattle (Simpson et al.,
1994).Ovulation rate has been shown to be
positively correlated with the peripheral
concentration of insulin and IGF-1 in cattle
(Gong et al., 1997). Increase in size of the
follicles (Simpson et al., 1994) and ovulation
rate (Cox et al., 1987) have been found in
animals treated with insulin. Insulin caused
recruitment of more gonadotropins responsive
follicles (Cox et al., 1987) and reduced atresia of
follicles (Matamoras et al., 1991).
Working with goats, Selvaraju et al
(2003) found that administration of purified
bovine insulin (0.2 IU/kg body weight per day)
on days 7, 8 and 9 of the estrus cycle resulted in
a significant increase in corpus leutum and
*
Part of Ph. D. Thesis submitted by the first author.
unovulated large follicles than the control group.
Thus, the use of insulin may be considered as an
alternative approach to improve superovulatory
response, and information on the influence of
insulin on ovulatory response in livestock is
scarce.Therefore the present investigation was
designed to study the effect of insulin treatment
on ovarian response in Karadi ewe lambs.
MATERIALS AND METHODS
Location of the experiment
The study was conducted on 25 Karadi ewes
aged one year old and 52 kg in weight at Animal
project, College of Agriculture, University of
Duhok.
Design of the experiment
The experimental animals originally
maintained on either 12 or 16 % crude protein
were divided randomly into two groups ; Group
1 (control, n=13 ) and Group 2 ( insulin
treatment , n=12 ).
At the start of the experiment, estrus was
induced by using intra vaginal sponges
containing MAP (Drug Facts and comparisons
2004, Tahran, Iran) for 14 days. At the time of
sponges withdrawn, animals were injected with
600 IU PMSG (LABORATORIOS