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The Influence Of Priming Two Cucumber Cultivar Seeds

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J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 139-147, 2010<br />

stored at room temperature for 32 days.This<br />

study investigated the effect of temperature and<br />

packaging materials on the quality of an orange<br />

juice during storage using models systems.<br />

941<br />

MATERIALS AND METHODS<br />

Model systems composition of local orange<br />

juice (Citrus sinesis Var.local) prepared<br />

according to the approach of Wong and<br />

Stanton(1989) after knowledge of components<br />

of local orange juice, as Sucrose 4.8% ,<br />

glucose 2.6% , fructose 2.6% , citric acid 1.1% ,<br />

potassium citrate 0.7% , aspartic acid 0.31% ,<br />

arginine 0.31% , ascorbic acid 0.043% , sodium<br />

benzoate 0.1% to prevent the growth of molds<br />

and yeasts(Gordon and Christine,1990) and<br />

manitol 0.44% to arrive the total soluble solids<br />

13%.<strong>The</strong> pH was adjusted with a citric acid<br />

solution to a value of 3.7 with pH –<br />

meter(HI98107).<strong>The</strong> juice was pasteurized at<br />

82 ° C for 15 min,and while still hot filled into<br />

glass bottles and plastic bottles with capacity<br />

130 ml leaving a headspace of about 10%.<strong>The</strong><br />

juice divided to four groups as glass bottles in<br />

the light,glass bottles in the dark,plastic bottles<br />

in the light and plastic bottles in the dark.All<br />

samples stored at 20,30 and 40 ° C for four<br />

months.<br />

<strong>The</strong> ascorbic acid concentration was<br />

measured for each month by the 2,6dichlorophenol<br />

indophenol according to the<br />

A<br />

B<br />

approach of (Rangama,1977),each sample was<br />

prepared and analysed in triplicate .Absorbance<br />

measurement at 420 nm was performed with<br />

spectrophotometer(TRSP-721) to evaluate<br />

nonenzymatic browning(Klim and Nagy,1988)<br />

for each month.Rate constant for ascorbic acid<br />

degradation were calculated through linear<br />

regression analysis of Ln[A] analog storage<br />

time(Nuray et al.,2003).Half time(t1/2)was<br />

calculate according to the equation which said<br />

by Samuel and Jerme(1974).<br />

K=constant.<br />

T1/2=Ln(2)/K.<br />

Activation energy(Ea)was calculate<br />

according to the equation which said by Samuel<br />

and Jerome(1974).<br />

Slope=Ea/(2.303*R).R(gas constant)=1.987 cal -<br />

1 .mol.<br />

Slope and intercept calculate from the Arrhenius<br />

plots shown in figure 1 (Nuray et al.,2003) using<br />

Microsoft Excel 2007.Shelf life calculate<br />

according to the equation observed by Al-<br />

Zubaidy and Khalil(2007).<br />

Expire=e -(s/T+I-Ln(-Ln)p/100))) .<br />

S=slope , T=kelven temperature , I=intercept ,<br />

P=residual (AA) .<br />

Data were statistically analyzed with SPSS<br />

2003,and the means vertifing were done<br />

according to Duncan , s multiple range test at<br />

level 1%.

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