The Influence Of Priming Two Cucumber Cultivar Seeds

J. Duhok Univ. Vol.13, No.1, (Agri. And Vet. Sciences) Pp 148-152, 2010
OVEJERO.S.A.Spain, then approved ram was
introduced 48 hrs later to detect the estrus. After
the occurrence of estrus in all ewes, the
following procedure was used as described by
Selvaraju et al (2003) All animals were
pretreated with 250IU Human chronic
gonadotropins ( Human Chorionic
gonadotropin : LG life sciences Korea) intra
muscularly on day 10 of the estrus cycle .Six
hours later , 1 mg estradiol -17 beta(Alburaihan
pharmaceutical Co. Tahran/Iran) dissolved in
olive oil was administrated subcutaneously,
Twenty – four hours after estradiol treatment the
animals were treated with 20 IU follicle
stimulating hormone (Laboratoires Serono
SA,CH-1170 Aubonne, Switzerland) im. in six
divided descending doses (4/4, 3/3, and 3/3) IU
at 12 h intervals for three consecutive days. All
animals were treated with PGF2 α analogue
(CEVA ANIMAL HEALTH Ltd, CHESHAM
HPS IGE-UK), 0.225 mg i.m. to induce
luteolysis along with the sixth FSH treatment
according to Majumdar et al. (1997). Prior to
gonadotropins administration , group 1 animals
were given a placebo of normal saline and
group 2 animals were given long acting purified
bovine insulin(Actrapid ,NOVO AIS,Denmark)
0.2 IU /Kg body weight per day s.c. for three
consecutive days started on day 7 of the estrus
cycle .
Estrus detection and breeding
After PGF2 α treatment, two rams fitted with
marking crayons were introduced placed with all
ewes and estrus was observed twice daily (at
morning and afternoon) . All ewes detected in
estrus were bred with natural service at
approximately 12 hrs interval until the end of
estrus.
Ova recovery
Three days following mating , all animals
were scarified to determine ovulation rate as
indicated by the presence of CL .The fallopian
tubes were flushed to recover ova .The weight
of left and right ovaries was recorded.
The diameter of corpus leutum was
measured. Also, the unovulated large follicles
and the white (a vascular) corpus leutum were
counted.
Statistical analysis
General linear model (GLM) within the
statistical model program SAS (2005) was used
to study the effect of insulin on ovary weight ,
number and diameter of corpora lutea ,ovulation
rate ,number of large follicle, number of white
corpora lutea and number of ova recovered ,
assuming the following model;
Yij = µ + Si + eij
Where,
Yij: measurements on j th observation;
µ : overall mean;
Si : effect of i th insulin level (i=Co, In); and
eij: random error NID (0 , Iσ 2 e).
Duncan Multiple Range Test (Duncan,
1955) also used to test the significant
differences between the levels of each
factor affecting the studied traits.
RESULTS AND DISCUSSIONS
All ewes in the two treatment groups
exhibited behavioral sign of estrus within three
days after PGF2α injection (Table 1). This result
is in accordance with those reported by Naqvi et
al. (1999). Weight of ovary averaged 2.31±0.15
and 2.06 ± 1.32 gm for the control and insulin
treated groups, respectively. Also, the diameter
of corpora lutea averaged 6.65±1.32 and
6.67±1.26 mm, respectively for control and
insulin treated group(Table 18). However, the
difference between groups was not significant
for both traits .
In the study reported herein, the percent of
ewes respond to treatment was 69.23% for
control group compared with 75.00% for insulin
treated group, and consequently the ovulation
rate indicated by the presence of corpora lutea
averaged 1.44 ±0.22 and 1.56±0.24 for the
control and insulin group, respectively (Table 1).
The mean ovulation rate obtained in the current
work is higher than 0.50-0.75 recorded for
pubertal Awassi ewe lambs (Jawad et al.1986),
for adult Awassi ewes (0.5-1.5) treated with the
different doses of GnRH (Alkass et al., 1989)
and for adult Awassi (0.8-1.2) imposed to
different feeding regimen (Alkass and Al-Kaisi,
1987). However, in general the low response of
ewes to superovulated by FSH is due to breed
and age of animals used. It appears that ewes
treated with insulin were stimulated to a greater
extent than control group as evident by number
of ovulations and unovulated large follicle.
However, the difference between the two groups
was not significant possibly due to limited
number of animals included in the present study
.
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