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Exotic Animal Formulary5

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Ethanol

10-30 mL 95%/L as

an immersion

to effect 12

Eugenol (a purified

derivative of clove

oil; also see clove oil)

Isoflurane/sevoflurane

≥17 mg/L as an

immersion to

effect 12

5-20 mL/L as an

immersion to

effect 12

Concentrations up to 5 times this amount

can be used; once the fish loses

consciousness, a secondary method

(double pithing, decapitation, injectable

pentobarbital) should be used

Due to the volatility of these comppounds

and risk to humans, ventilation

precautions should be taken

Ketamine 66-88 mg/kg IM 12 Follow with a lethal pentobarbital injection

Ketamine

(K)/dexmedetomidine

(D)

2-phenoxyethanol

(K) 1-2 mg/kg+(D)

0.05-0.1 mg/kg

IM 12

≥0.5-0.6 mL/L or

0.3-0.4 mg/L as

an immersion

to effect 12

Follow with a lethal pentobarbital injection

Propofol 1.5-2.5 mg/L IM 12 Follow with a lethal pentobarbital injection

Quinaldine sulfate

Tricaine

methanesulfonate

(MS-222) (Tricaine-S,

Syndel USA)

5-10 mg/L as an

immersion to

effect 103

≥100 mg/L as an

immersion to

effect 12

250-500 mg/L as an

immersion for

at least 10 min

after cessation

of respiration 12

Once the fish loses consciousness, a

secondary method (double pithing,

decapitation, injectable intracardiac

pentobarbital) should be used

Buffering may be required in some cases

Buffering is required and a secondary method

(double pithing, decapitation, injectable

intracardiac pentobarbital) should be used

a

Not to be used in fish for human consumption; CO 2 euthanasia is the exception.

Table 2-6

Hematologic and Serum Biochemical Values of Fish. a

Measurement

Goldfish (Carassius

auratus) 49 Koi (Cyprinus carpio) 49,107,150

Hematology

69

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