Proceedings of the 10th International Colloquium on Paratuberculosis

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Monday, August 10, 2009 – All sessions held in Willey Hall 175 0800-0830 COFFEE and TEA service in Willey Hall atrium (complimentary hot breakfast served in Middlebrook Hall for all attendees regardless <strong>of</strong> lodging choice) 0900-0930 Diagnostics & Genotyping Keynote: Richard Whittington – Diagnostic methods for identification and characterization <strong>of</strong> M. avium subsp. paratuberculosis: advances, constraints and opportunities. 0930-0950 #229: Detecting Johne's Disease heavy shedders in dairy cows. Luis A Espejo, Scott J Wells 0950-1010 #205: Sensitivity <strong>of</strong> environmental sampling for paratuberculosis. Maarten F Weber, Kees van Maanen, Thea von Bannisshet-Wijsmuller, Theo J G M Lam 1010-1030 1030 #231: Rapid, sensitive detection <strong>of</strong> MAP in milk using a phage-based method. George Botsaris, Maria Liapi, Charalambos Kakogiannis, Christine Dodd, Ca<strong>the</strong>rine Rees Refreshment break 1040-1100 #101: Using liquid and agar-based methods to investigate <strong>the</strong> bacteriostatic activity <strong>of</strong> lactic acid bacteria and <strong>the</strong>ir metabolites against Mycobacterium avium subsp. paratuberculosis. John Anthony Donaghy, Jane Johnston, Michael Rowe 9
1100-1120 #107: Multilocus short sequence repeat analysis <strong>of</strong> Mycobacterium avium subsp. paratuberculosis isolates from dairy herds in nor<strong>the</strong>astern United States <strong>of</strong> a longitudinal study indicates low shedders are truly infected. Abani K Pradhan, Aagje J Kramer, Rebecca M Mitchell, Robert H Whitlock, Julie M Smith, Ernest Hovingh, Jo Ann S Van Kessel, Jeff S Karns, Ynte H Schukken 1120-1140 #105: MIRU-VNTR genotyping <strong>of</strong> Mycobacterium avium subspecies paratuberculosis revealed Type-specific differences at two component regulatory systems. Elena Castellanos, Beatriz Romero, Sabrina Rodriguez, Javier Bezos, Lucia de Juan, Ana Mateos, Lucas Dominguez, Alicia Aranaz 1200 -1230 Perspectives: Ivo Palvik – Genotyping perspectives 1230-1430 LUNCH – Willey atrium and outdoor courtyard 1430-1500 Host Response and Immunology Keynote: Judy Stabel – Unraveling <strong>the</strong> Host Response to Mycobacterium avium subsp. paratuberculosis:One Thread at a Time. 1500-1520 #172: Whole genome association study for Holstein susceptibility to MAP infection Brian W Kirkpatrick, Xianwei Shi, Michael Collins, George E Shook 1520-1540 Merkal Award - #33: No interference <strong>of</strong> heat shock protein 70 subunit vaccination against bovine paratuberculosis. Wiebren Santema, Selma Hensen, Victor Rutten, Ad Koets 1540-1600 #68: Neutralisation <strong>of</strong> interleukin-10 from CD14+ monocytes enhances gamma interferon production in peripheral blood mononuclear cell from mycobacterium avium subsp. paratuberculosis infected goats. Kari Roste Lybeck, Anne Kristine Storset, Ingrid Olsen 10
Page 1 and 2: Book of Abstracts
Page 4 and 5: Proceedings <stron
Page 6 and 7: Grant Support and Sponsorship <stro
Page 8 and 9: Scott Wells - Planning Committee Ch
Page 12 and 13: Kari Røste Lybeck, Anne Kristine S
Page 14 and 15: 1120-1140 #88: Influence of
Page 16 and 17: Concurrent Session B - Rm 175 Wille
Page 18 and 19: Thursday, August 13 Thursday, Augus
Page 20: Diagnostics and Genotyping Convenor
Page 23 and 24: #229 Detecting Johne’s Disease he
Page 25 and 26: Kalis CHJ, Hesselink JW, Barkema HW
Page 27 and 28: MATERIALS AND METHODS Sampling For
Page 29 and 30: To better determine the</st
Page 31 and 32: #107 Multilocus Short Sequence Repe
Page 33 and 34: Fig. 1. Dendogram (showing genetic
Page 35 and 36: #105 MIRU-VNTR genotyping o
Page 37: Diagnostics and Genotyping Poster A
Page 40 and 41: Missouri). Serum from a cow with do
Page 42 and 43: #7 Significance of
Page 44 and 45: RESULTS Of the 327
Page 46 and 47: Direct fecal nested Map PCR testing
Page 48 and 49: #16 Histopathological and immunohis
Page 50 and 51: #19 Development of
Page 52 and 53: #36 Genetic diversity of</s
Page 54 and 55: #60 Evaluation of
Page 56 and 57: #66 Comparison of
Page 58 and 59: (b) IS900 Nested PCR, using p90-p91
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(d) f57 Real Time PCR. The figure o
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• MIRU (3 loci); • VNTR-MIRU (7
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indexes, compared to each single an
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#108 Evaluation of
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#110 Detection and molecular confir
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endoscopic criteria and histopathol
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Bull TJ, McMinn EJ, Sidi-Boumedine
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#131 Seroprevalence of</str
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could be partly attributed to a sma
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#146 Analysis of v
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#162 Comparison of
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#187 Comparison of
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#191 Potentiating day-old blood sam
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samples from the s
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(MAA) (MAP87 and MAP3776) and 1 fro
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#196 Inter- and intra-subtype genot
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RESULTS In 601 (29%) of</st
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#211 Culture of my
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#242 Application and field validati
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#251 Diagnosis of
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Table 2. Paratuberculosis PCR resul
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#254 Programmes on Paratuberculosis
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Fig. 1. European countries specifyi
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A bulk milk quality assurance progr
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Republic of Lithua
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#276 Elimination of</strong
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other projects and
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Woodbine KA, Schukken YH, Green LE,
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Host Response and Immunology Keynot
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#33 MERKAL AWARD LECTURE No interfe
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#75 Interleukin 17 and interleukin
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#173 Local and systemic roles for b
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#23 Role of Mycoba
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Host Response and Immunology Poster
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#28 Intestinal MAP Infection via Pe
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#73 Age susceptibility of</
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#95 Role of nitric
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#134 Analysis of <
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#157 Study of <str
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#163 Immunohistochemical expression
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#193 Development of</strong
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#209 Murine macrophages carrying an
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#224 Application of</strong
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Table 1 UK and Cyprus MAP survey Re
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#228 Mycobacterium avium ssp. parat
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#145 MERKAL AWARD LECTURE Multinomi
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#50 Assessment of
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Table 1. Posterior median (CrIs) <s
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#55 Birth clusters of</stro
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from their dam, se
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#88 Influence of b
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#104 Relation between faecal shedde
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Epidemiology Poster Abstracts 109 1
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#22 The Prevalence of</stro
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CONCLUSION IS1311-based nested Ma/M
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#42 Seroprevalence survey o
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#77 Characterisation of</st
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Environmental samples were collecte
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REFERENCES 1) Chiodini RJ, Van Krui
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#113 Effect of soi
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#136 Error-adjusted, variance parti
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Latium Region Viterbo districts RES
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#144 The suitability of</st
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#178 Age structure of</stro
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A statistically significant lower f
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#183 Sero-prevalence of</st
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#218 Johne’s disease in t
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#244 A survey to estimate t
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Control Programs Keynote Lecture Re
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#202 Milk quality assurance for par
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#141 Control of pa
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#198 Use of small
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Control Programs Poster Abstracts 1
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RESULTS Loss of al
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#40 Managing Bovine Johnes Disease
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#74 Economic analysis of</s
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#87 Evaluation of
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separately for each existing herd <
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#98 An inter-laboratory ring-trial
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#120 Paratuberculosis vaccine inter
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The statement provides: • A stand
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COMPLEMENTARY ASPECTS On-Farm Disea
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#168 Paratuberculosis in Iran: past
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#180 Behavioural change in owners <
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the fact that <str
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#182 An integrated risk based appro
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etween 0 to 10, depending on <stron
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#203 Milk quality assurance for par
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#234 Johne’s disease vaccine: a c
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Pathogenomics and MAP Biology Conve
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#45 Identification of</stro
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#117 Adsorption of
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#222 Atypical structural features <
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Pathogenomics and MAP Biology Persp
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247
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#52 Is ‘Indian Bison Type’ Myco
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#69 In vitro susceptibility <strong
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#118 Cloning, expression and purifi
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#176 A reference proteomic pr<stron
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#213 Construction the</stro
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#230 Iron concentration dependent s
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261
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263
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Abstract not available at press tim
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#174 Sharing of
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#177 Associations between CARD15 po
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Perspectives Talk: Public Health My
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273
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A total of 206 qua
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Feller, M., K. Huwiler, R. Stephan,
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In 2008, the Ameri
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#115 Crohn’s disease and ruminant
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International John
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#76 Towards improved reporting stan
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#97 The EU ParaTBTools Project - Th
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#241 US Vaccine Initiative through
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Paratuberculosis ELISA Reliable, si
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PRIONICS AG WAGISTRASSE 27A PHONE +
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