Proceedings of the 10th International Colloquium on Paratuberculosis

More documents

Recommendations

Info

Missouri). Serum from a cow with documented Johne’s disease constituted <strong>the</strong> positive control. Final analytical readings were done at 24 and 48 hours. The appearance <strong>of</strong> one or more clearly definable precipitation lines before or at 48 hours constituted a positive result. The AGID tests were done with both nonabsorded and Mycobacterium phlei absorbed sera. Pre-absorbed ELISA test: Test sera were pre-absorbed with M. phlei. The ELISA results were calculated from wavelength readings at optical density (OD) 405 nm. All readings less than 1.6 OD were considered negative; readings between 1.5 and 1.9 OD were deemed suspicious/inconclusive; and readings <strong>of</strong> 2.0 to 2.5 OD were called low positive. A high positive was any reading 2.6 OD or above. RESULTS Frequency <strong>of</strong> double precipitation bands within <strong>the</strong> AGID test: Of <strong>the</strong> 71 individual animals identified having a positive AGID test, 13 (18%) exhibited, at one point or ano<strong>the</strong>r, a double immunoprecipitation band. Relationship <strong>of</strong> double precipitation bands to corresponding ELISA optical density readings: The mean ELISA optical density reading (OD) for <strong>the</strong> 13 cows whose AGID test demonstrated a double precipitation band was 3.88 with a range from 2.3 to 5.8. Only one serum had an OD reading <strong>of</strong> less than 2.6. Single, primary precipitation bands were not tightly correlated with <strong>the</strong> presence <strong>of</strong> a diagnostic Map ELISA test. Nine <strong>of</strong> <strong>the</strong> 71 (12.7%) cases in which a double band occurred had a negative UF ELISA test. Ano<strong>the</strong>r 10 (14%) sera were deemed to be, at best, suspicious (Table 1). The greatest number <strong>of</strong> cases correlated with strongly positive Map ELISA tests 39/71 (54.9%). Serial observation in three cows demonstrated <strong>the</strong> unmasking <strong>of</strong> a second immunoprecipitation band when <strong>the</strong> corresponding ELISA titer became markedly elevated above that value at which <strong>the</strong> primary immunoprecipitation band occurred (Table 2) When AGID positive sera were absorbed against varying concentration <strong>of</strong> M. plea, <strong>the</strong> results for <strong>the</strong> immunoprecipitation bands remained unchanged. Table 1. Distribution <strong>of</strong> serum Map ELISA titers among Holstein cows with a positive AGID test ELISA Titer Number <strong>of</strong> Cows (Percentage) 0 – 1.5 – negative 9 (12.7%) 1.6 – 1.9 – suspicious 10 (14%) 2.0 – 2.5 - positive 13 (18.3%) Greater than 2.5 – strong positive 39 (54.9%) DISCUSSION The common absence <strong>of</strong> an immunoprecipitation band in <strong>the</strong> face <strong>of</strong> a high Map ELISA test result has been largely ascribed to <strong>the</strong> amount <strong>of</strong> specific antibody required for an immunoprecipitation band in contrast to <strong>the</strong> significantly smaller amount <strong>of</strong> specific antibody that is required for a diagnostic Map ELISA reading. Unlike <strong>the</strong> primary immunoprecipitation band, <strong>the</strong> second immunoprecipitation band appears to have a positive correlation with markedly elevated Map ELISA test readings. This observation implies that <strong>the</strong> second immunoprecipitation band identifies predominantly <strong>the</strong> same Map antigen complexes that are used in <strong>the</strong> Map ELISA test. The resultant interpretation <strong>of</strong> this data was that a better Map ELISA test could be developed using selected antigenic subsets embedded in <strong>the</strong> primary immunoprecipitation band. 39
Table 2. ELISA Optical Density Readings in Cows with Double AGID Precipitation Bands in One or More Sera Date AGID ELISA Cow #3917 8/21/03 double band 5.5 8/25/03 double band 5.0 8/28/03 double band 4.1 9/2/03 single band 3.9 9/3/04 single band 3.4 9/4/03 Cow #3882 single band 3.6 single band 3.49 double band 4.96 single band 3.09 single band 4.72 Cow #4151 single band 2.32 double band 4.1 REFERENCES 1. Sockett D.C., Conrad T.A., Thomas C.B. et al.: 1992. Evaluation <strong>of</strong> four serological tests for bovine paratuberculosis. J.Clin. Microbiol. 30:1134-1139. 2. McNab WB, Meek AH, Duncan JR, et al.: 1991. An evaluation <strong>of</strong> selected screening tests for bovine paratuberculosis. Can J Vet Med 55:252-259. 3. Sergeant E.S. G., Marshall D.J., Eamens G.J. et al.: 2003. Evaluation <strong>of</strong> an absorbed ELISA and an agar-gel immunodiffusion tests in ovine paratuberculosis in sheep in Australia. Prevent. Vet. Med. 61:235-248. 4. Williams E., Monif G. R. G., Buergelt C. D.: 2008. Comparative analysis <strong>of</strong> different Map ELISA tests. The Paratuberculosis Newsletter- March: p.7. 40
Page 1 and 2: Book of Abstracts
Page 4 and 5: Proceedings <stron
Page 6 and 7: Grant Support and Sponsorship <stro
Page 8 and 9: Scott Wells - Planning Committee Ch
Page 10 and 11: Monday, August 10, 2009 - All sessi
Page 12 and 13: Kari Røste Lybeck, Anne Kristine S
Page 14 and 15: 1120-1140 #88: Influence of
Page 16 and 17: Concurrent Session B - Rm 175 Wille
Page 18 and 19: Thursday, August 13 Thursday, Augus
Page 20: Diagnostics and Genotyping Convenor
Page 23 and 24: #229 Detecting Johne’s Disease he
Page 25 and 26: Kalis CHJ, Hesselink JW, Barkema HW
Page 27 and 28: MATERIALS AND METHODS Sampling For
Page 29 and 30: To better determine the</st
Page 31 and 32: #107 Multilocus Short Sequence Repe
Page 33 and 34: Fig. 1. Dendogram (showing genetic
Page 35 and 36: #105 MIRU-VNTR genotyping o
Page 37: Diagnostics and Genotyping Poster A
Page 42 and 43: #7 Significance of
Page 44 and 45: RESULTS Of the 327
Page 46 and 47: Direct fecal nested Map PCR testing
Page 48 and 49: #16 Histopathological and immunohis
Page 50 and 51: #19 Development of
Page 52 and 53: #36 Genetic diversity of</s
Page 54 and 55: #60 Evaluation of
Page 56 and 57: #66 Comparison of
Page 58 and 59: (b) IS900 Nested PCR, using p90-p91
Page 60 and 61: (d) f57 Real Time PCR. The figure o
Page 62 and 63: • MIRU (3 loci); • VNTR-MIRU (7
Page 64 and 65: indexes, compared to each single an
Page 66 and 67: #108 Evaluation of
Page 68 and 69: #110 Detection and molecular confir
Page 70 and 71: endoscopic criteria and histopathol
Page 72 and 73: Bull TJ, McMinn EJ, Sidi-Boumedine
Page 74 and 75: #131 Seroprevalence of</str
Page 76 and 77: could be partly attributed to a sma
Page 78 and 79: #146 Analysis of v
Page 84 and 85: #191 Potentiating day-old blood sam
Page 86 and 87: samples from the s
Page 88 and 89: (MAA) (MAP87 and MAP3776) and 1 fro
Page 90 and 91:
#196 Inter- and intra-subtype genot
Page 92 and 93:
RESULTS In 601 (29%) of</st
Page 94 and 95:
#211 Culture of my
Page 96 and 97:
#242 Application and field validati
Page 98 and 99:
#251 Diagnosis of
Page 100 and 101:
Table 2. Paratuberculosis PCR resul
Page 102 and 103:
#254 Programmes on Paratuberculosis
Page 104 and 105:
Fig. 1. European countries specifyi
Page 106 and 107:
A bulk milk quality assurance progr
Page 108 and 109:
Republic of Lithua
Page 110 and 111:
#276 Elimination of</strong
Page 112 and 113:
other projects and
Page 114 and 115:
Woodbine KA, Schukken YH, Green LE,
Page 116 and 117:
Host Response and Immunology Keynot
Page 118 and 119:
#33 MERKAL AWARD LECTURE No interfe
Page 120 and 121:
#75 Interleukin 17 and interleukin
Page 122 and 123:
#173 Local and systemic roles for b
Page 124 and 125:
#23 Role of Mycoba
Page 126 and 127:
Host Response and Immunology Poster
Page 128 and 129:
#28 Intestinal MAP Infection via Pe
Page 130 and 131:
#73 Age susceptibility of</
Page 132 and 133:
#95 Role of nitric
Page 134 and 135:
#134 Analysis of <
Page 136 and 137:
#157 Study of <str
Page 138 and 139:
#163 Immunohistochemical expression
Page 140 and 141:
#193 Development of</strong
Page 142 and 143:
#209 Murine macrophages carrying an
Page 144 and 145:
#224 Application of</strong
Page 146 and 147:
Table 1 UK and Cyprus MAP survey Re
Page 148 and 149:
#228 Mycobacterium avium ssp. parat
Page 150 and 151:
#145 MERKAL AWARD LECTURE Multinomi
Page 152 and 153:
#50 Assessment of
Page 154 and 155:
Table 1. Posterior median (CrIs) <s
Page 156 and 157:
#55 Birth clusters of</stro
Page 158 and 159:
from their dam, se
Page 160 and 161:
#88 Influence of b
Page 162 and 163:
#104 Relation between faecal shedde
Page 164 and 165:
Epidemiology Poster Abstracts 109 1
Page 166 and 167:
#22 The Prevalence of</stro
Page 168 and 169:
CONCLUSION IS1311-based nested Ma/M
Page 170 and 171:
#42 Seroprevalence survey o
Page 172 and 173:
#77 Characterisation of</st
Page 174 and 175:
Environmental samples were collecte
Page 176 and 177:
REFERENCES 1) Chiodini RJ, Van Krui
Page 178 and 179:
#113 Effect of soi
Page 180 and 181:
#136 Error-adjusted, variance parti
Page 182 and 183:
Latium Region Viterbo districts RES
Page 184 and 185:
#144 The suitability of</st
Page 186 and 187:
#178 Age structure of</stro
Page 188 and 189:
A statistically significant lower f
Page 190 and 191:
#183 Sero-prevalence of</st
Page 192 and 193:
#218 Johne’s disease in t
Page 194 and 195:
#244 A survey to estimate t
Page 196 and 197:
Control Programs Keynote Lecture Re
Page 198 and 199:
#202 Milk quality assurance for par
Page 200 and 201:
#141 Control of pa
Page 202 and 203:
#198 Use of small
Page 204 and 205:
Control Programs Poster Abstracts 1
Page 206 and 207:
RESULTS Loss of al
Page 208 and 209:
#40 Managing Bovine Johnes Disease
Page 210 and 211:
#74 Economic analysis of</s
Page 212 and 213:
#87 Evaluation of
Page 214 and 215:
separately for each existing herd <
Page 216 and 217:
#98 An inter-laboratory ring-trial
Page 218 and 219:
#120 Paratuberculosis vaccine inter
Page 220 and 221:
The statement provides: • A stand
Page 222 and 223:
COMPLEMENTARY ASPECTS On-Farm Disea
Page 224 and 225:
#168 Paratuberculosis in Iran: past
Page 226 and 227:
#180 Behavioural change in owners <
Page 228 and 229:
the fact that <str
Page 230 and 231:
#182 An integrated risk based appro
Page 232 and 233:
etween 0 to 10, depending on <stron
Page 234 and 235:
#203 Milk quality assurance for par
Page 236 and 237:
#234 Johne’s disease vaccine: a c
Page 238 and 239:
Pathogenomics and MAP Biology Conve
Page 240 and 241:
#45 Identification of</stro
Page 242 and 243:
#117 Adsorption of
Page 244 and 245:
#222 Atypical structural features <
Page 246 and 247:
Pathogenomics and MAP Biology Persp
Page 248 and 249:
247
Page 250 and 251:
#52 Is ‘Indian Bison Type’ Myco
Page 252 and 253:
#69 In vitro susceptibility <strong
Page 254 and 255:
#118 Cloning, expression and purifi
Page 256 and 257:
#176 A reference proteomic pr<stron
Page 258 and 259:
#213 Construction the</stro
Page 260 and 261:
#230 Iron concentration dependent s
Page 262 and 263:
261
Page 264 and 265:
263
Page 266 and 267:
Abstract not available at press tim
Page 268 and 269:
#174 Sharing of
Page 270 and 271:
#177 Associations between CARD15 po
Page 272 and 273:
Perspectives Talk: Public Health My
Page 274 and 275:
273
Page 276 and 277:
A total of 206 qua
Page 278 and 279:
Feller, M., K. Huwiler, R. Stephan,
Page 280 and 281:
In 2008, the Ameri
Page 282 and 283:
#115 Crohn’s disease and ruminant
Page 284 and 285:
International John
Page 286 and 287:
#76 Towards improved reporting stan
Page 288 and 289:
#97 The EU ParaTBTools Project - Th
Page 290 and 291:
#241 US Vaccine Initiative through
Page 292 and 293:
Paratuberculosis ELISA Reliable, si
Page 294 and 295:
PRIONICS AG WAGISTRASSE 27A PHONE +
show all

Proceedings of the 10th International Colloquium on Paratuberculosis

Create successful ePaper yourself

Delete template?

Save as template?