Proceedings of the 10th International Colloquium on Paratuberculosis

A statistically significant lower frequency in MAP infection was observed in animals
older than 5 years compared to 3-5 years-old animals (pELISA=0.0002; pCULTURE=0.0087; pRTi-
PCR=0.0078; pHP< 0.0001).
A. B.
NUMBER OF SAMPLES (n)
140
120
100
80
60
40
20
0
25.0
10.4
16.5
10.8
5.9
3.0
4.0
7.4
11.1
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11
AGE (YEARS)
C. D.
NUMBER OF SAMPLES (n)
140
120
100
80
60
40
20
0
25.0
18.7
26.8
25.0
18.8
13.4
10.9
12.0
22.2
11.1
26.3
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11
AGE (YEARS)
45
40
35
30
25
20
15
10
5
0
45
40
35
30
25
20
15
10
5
0
PTB PREVALENCE (%) .
PTB PREVALENCE (%) .
NUMBER OF SAMPLES (n)
140
120
100
80
60
40
20
0
140
120
100
80
60
40
20
0
25.0
18.7
27.1
21.8
18.8
8.9 9.1
12.0
23.1
16.7
23.5
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11
33.3
18.7
42.3
25.0
AGE (YEARS)
6.1
4.0
14.3
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11
AGE (YEARS)
Fig. 1. Age structure <strong>of</strong> MAP infection in relation to diagnostic tests: ELISA (A), culture (B),
RTi-PCR (C) and histopathological examination (D). Line: MAP prevalence and bars: number
<strong>of</strong> samples tested.
Table 1. Sensitivity, complementary sensitivity and agreement (�) values <strong>of</strong> diagnostic test
combinations. Interpretation <strong>of</strong> agreement: poor (�=0.00-0.20), fair (�=0.21-0.40), moderate
(�=0.41-0.60), good (�=0.61-0.80) and excellent (�=0.81-1.00).
Sensitivity (%) Complementary sensitivity (%)
EVALUATED
REFERENCE TEST
REFERENCE TEST
TEST ELISA CULTURE RTi-PCR HP ELISA CULTURE RTi-PCR HP
ELISA
CULTURE
RTi-PCR
NUMBER OF SAMPLES (n)
- 37.0 37.1 72.4 - 6.5 4.3 24.1
(�=0.454) (�=0.461) (�=0.696)
85.1 - 63.4 85.7 144.7 - 33.0 92.9
(�=0.562) (�=0.536)
89.6 65.7 - 86.2 152.0 38.0 - 96.5
(�=0.525)
HP 75.0 48.0 47.2 - 28.6 8.0 7.5 -
Broadly, diagnostic test combinations resulted in moderate agreement (�=0.41-0.60)
excepting for <strong>the</strong> combination ELISA and histopathological examination that showed a good
187
45
40
35
30
25
20
15
10
5
0
45
40
35
30
25
20
15
10
5
0
PTB PREVALENCE (%) .
PTB PREVALENCE (%) .