Proceedings of the 10th International Colloquium on Paratuberculosis

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REFERENCES 1) Chiodini RJ, Van Kruiningen HJ, Merkal RS, 1984. Ruminant paratuberculosis (Johne’s disease): <strong>the</strong> current status and future prospetcs. Cornell Vet., 74, 218-262. 2) Grewal SK, Rajeev S, Sreevatsan S, Frederick CM, 2006. Persistence <strong>of</strong> Mycobacterium avium subsp. paratuberculosis and o<strong>the</strong>r zoonotic pathogens during simulate composting, manure packing, and liquid storage <strong>of</strong> dairy manure. Appl. Env. Microb. 72, 1, 565-574. 3) Obasanjo IO, Grohn YT, Mohammed HO, 1997.. Farm factors associated with <strong>the</strong> presence <strong>of</strong> Mycobacterium paratuberculosis infection in dairy herds on <strong>the</strong> New York State Paratuberculosis Control Program. Prev.Vet.Med. 32, 3-4, 254-251. 4) Raizman EA, Wells SJ, Godden SM, Bey RF, Oakes MJ, Bentley DC, Olsen KE, 2004. The distribution <strong>of</strong> Mycobacterium avium ssp. paratuberculosis in <strong>the</strong> environment surrounding Minnesota dairy farms. J. Dairy Sci. 87, 2959-2966. 5) Taddei R, Belletti GL, Beltrami A, Tamba M, Arrigoni N, 2006. Ricerca di Mycobacterium avium subsp. paratuberculosis (Map) da feci bovine: confronto tra quattro differenti metodi PCR. Atti VIII Congresso Naz. S.I.Di.L.V., Perugia, 9-10/11/2006, 37-38. 6) Taddei S, Robbi C, Cesena C, Rossi I, Schiano E, Arrigoni N, Vicenzoni G, Cavirani S, 2004.. Detection <strong>of</strong> Mycobacterium avium subs. paratuberculosis in bovine fecal samples: comparison <strong>of</strong> three polymerase chain reaction- based diagnostic tests with a conventional culture method. J. Vet. Diagn. Invest., 16, 503-508. 7) Jørgensen JB, 1977. Survival <strong>of</strong> Mycobacterium paratuberculosis in slurry. Nord. Vet. Med., 29, 267-270. 175 4
#111 Detection and molecular confirmation <strong>of</strong> Mycobacterium avium subsp. paratuberculosis in drainage water and forage after application <strong>of</strong> dairy cattle manure on agricultural soils Miguel Angel Salgado, Marta Andrea Alfaro, Francisco Javier Salazar, Robert Söderlund, Göran Bölske, Monica Viviana Pradenas, Armin Fabian Mella, Juan Kruze Universidad Austral de Chile, Chile; Institute for Agricultural Research, Chile; National Veterinary Institute (SVA), Sweden Mycobacterium avium subsp. paratuberculosis (Map) is considered an obligate pathogen. In <strong>the</strong>ory it can be eradicated by <strong>the</strong> removal <strong>of</strong> all infected animals. However, Map can survive for long periods outside <strong>the</strong> host. Land spreading <strong>of</strong> manure can cause water pollution, ei<strong>the</strong>r through direct aerosol contamination or through run<strong>of</strong>f from manure applied on <strong>the</strong> fields. There is no published data on Map survival in soils after manure application. The aim <strong>of</strong> this study was to detect <strong>the</strong> presence <strong>of</strong> Map after application <strong>of</strong> contaminated manure onto agricultural soils. A longitudinal study was carried out by extracting intact soil columns that were kept under controlled environmental conditions. Spiked manure with a known bacterial concentration (108 Map/ml) and manure from Map positive animals was applied to <strong>the</strong> soil and both drainage water and forage samples were analyzed bacteriologically. An automated liquid culture system (MGIT) was used for Map detection. All positive tubes were confirmed by a robust real-time PCR for IS900 and F57 targets, including effective mechanical cell disruption, DNA extraction and removal <strong>of</strong> PCR inhibitors. Preliminary results show that Map could be detected by MGIT and confirmed by IS900 and F57 PCR in drainage water 2 to 4 months after manure application. Map presence in forage and top soil could be confirmed 8 months after manure application. Regarding <strong>the</strong> cycle threshold values (Ct) from <strong>the</strong> real-time PCR (where <strong>the</strong> Ct cut-<strong>of</strong>f value is above 40 for a negative result), positive drainage water samples showed on average 33 Ct, compared with 25 Ct <strong>of</strong> positive samples from forage and top soil. These results are suggestive <strong>of</strong> forage and top soil being a more favourable environment for Map survival than can be provided by <strong>the</strong> subsoil, implying a higher risk for grazing animals than for pollution <strong>of</strong> well water. 176
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Book of Abstracts
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Proceedings <stron
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Grant Support and Sponsorship <stro
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Scott Wells - Planning Committee Ch
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Monday, August 10, 2009 - All sessi
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Kari Røste Lybeck, Anne Kristine S
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1120-1140 #88: Influence of
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Concurrent Session B - Rm 175 Wille
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Thursday, August 13 Thursday, Augus
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Diagnostics and Genotyping Convenor
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#229 Detecting Johne’s Disease he
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Kalis CHJ, Hesselink JW, Barkema HW
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MATERIALS AND METHODS Sampling For
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To better determine the</st
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#107 Multilocus Short Sequence Repe
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Fig. 1. Dendogram (showing genetic
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#105 MIRU-VNTR genotyping o
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Diagnostics and Genotyping Poster A
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Missouri). Serum from a cow with do
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#7 Significance of
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RESULTS Of the 327
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Direct fecal nested Map PCR testing
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#16 Histopathological and immunohis
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#19 Development of
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#36 Genetic diversity of</s
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#60 Evaluation of
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#66 Comparison of
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(b) IS900 Nested PCR, using p90-p91
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(d) f57 Real Time PCR. The figure o
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• MIRU (3 loci); • VNTR-MIRU (7
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indexes, compared to each single an
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#108 Evaluation of
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#110 Detection and molecular confir
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endoscopic criteria and histopathol
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Bull TJ, McMinn EJ, Sidi-Boumedine
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#131 Seroprevalence of</str
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could be partly attributed to a sma
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#146 Analysis of v
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#162 Comparison of
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#187 Comparison of
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#191 Potentiating day-old blood sam
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samples from the s
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(MAA) (MAP87 and MAP3776) and 1 fro
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#196 Inter- and intra-subtype genot
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RESULTS In 601 (29%) of</st
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#211 Culture of my
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#242 Application and field validati
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#251 Diagnosis of
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Table 2. Paratuberculosis PCR resul
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#254 Programmes on Paratuberculosis
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Fig. 1. European countries specifyi
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A bulk milk quality assurance progr
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Republic of Lithua
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#276 Elimination of</strong
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other projects and
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Woodbine KA, Schukken YH, Green LE,
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Host Response and Immunology Keynot
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#33 MERKAL AWARD LECTURE No interfe
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#75 Interleukin 17 and interleukin
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#173 Local and systemic roles for b
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#23 Role of Mycoba
Page 126 and 127: Host Response and Immunology Poster
Page 128 and 129: #28 Intestinal MAP Infection via Pe
Page 130 and 131: #73 Age susceptibility of</
Page 132 and 133: #95 Role of nitric
Page 134 and 135: #134 Analysis of <
Page 136 and 137: #157 Study of <str
Page 138 and 139: #163 Immunohistochemical expression
Page 140 and 141: #193 Development of</strong
Page 142 and 143: #209 Murine macrophages carrying an
Page 144 and 145: #224 Application of</strong
Page 146 and 147: Table 1 UK and Cyprus MAP survey Re
Page 148 and 149: #228 Mycobacterium avium ssp. parat
Page 150 and 151: #145 MERKAL AWARD LECTURE Multinomi
Page 152 and 153: #50 Assessment of
Page 154 and 155: Table 1. Posterior median (CrIs) <s
Page 156 and 157: #55 Birth clusters of</stro
Page 158 and 159: from their dam, se
Page 160 and 161: #88 Influence of b
Page 162 and 163: #104 Relation between faecal shedde
Page 164 and 165: Epidemiology Poster Abstracts 109 1
Page 166 and 167: #22 The Prevalence of</stro
Page 168 and 169: CONCLUSION IS1311-based nested Ma/M
Page 170 and 171: #42 Seroprevalence survey o
Page 172 and 173: #77 Characterisation of</st
Page 174 and 175: Environmental samples were collecte
Page 178 and 179: #113 Effect of soi
Page 180 and 181: #136 Error-adjusted, variance parti
Page 182 and 183: Latium Region Viterbo districts RES
Page 184 and 185: #144 The suitability of</st
Page 186 and 187: #178 Age structure of</stro
Page 188 and 189: A statistically significant lower f
Page 190 and 191: #183 Sero-prevalence of</st
Page 192 and 193: #218 Johne’s disease in t
Page 194 and 195: #244 A survey to estimate t
Page 196 and 197: Control Programs Keynote Lecture Re
Page 198 and 199: #202 Milk quality assurance for par
Page 200 and 201: #141 Control of pa
Page 202 and 203: #198 Use of small
Page 204 and 205: Control Programs Poster Abstracts 1
Page 206 and 207: RESULTS Loss of al
Page 208 and 209: #40 Managing Bovine Johnes Disease
Page 210 and 211: #74 Economic analysis of</s
Page 212 and 213: #87 Evaluation of
Page 214 and 215: separately for each existing herd <
Page 216 and 217: #98 An inter-laboratory ring-trial
Page 218 and 219: #120 Paratuberculosis vaccine inter
Page 220 and 221: The statement provides: • A stand
Page 222 and 223: COMPLEMENTARY ASPECTS On-Farm Disea
Page 224 and 225: #168 Paratuberculosis in Iran: past
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#180 Behavioural change in owners <
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the fact that <str
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#182 An integrated risk based appro
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etween 0 to 10, depending on <stron
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#203 Milk quality assurance for par
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#234 Johne’s disease vaccine: a c
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Pathogenomics and MAP Biology Conve
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#45 Identification of</stro
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#117 Adsorption of
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#222 Atypical structural features <
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Pathogenomics and MAP Biology Persp
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#52 Is ‘Indian Bison Type’ Myco
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#69 In vitro susceptibility <strong
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#118 Cloning, expression and purifi
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#176 A reference proteomic pr<stron
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#213 Construction the</stro
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#230 Iron concentration dependent s
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Abstract not available at press tim
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#174 Sharing of
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#177 Associations between CARD15 po
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Perspectives Talk: Public Health My
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273
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A total of 206 qua
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Feller, M., K. Huwiler, R. Stephan,
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In 2008, the Ameri
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#115 Crohn’s disease and ruminant
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International John
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#76 Towards improved reporting stan
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#97 The EU ParaTBTools Project - Th
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#241 US Vaccine Initiative through
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Paratuberculosis ELISA Reliable, si
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PRIONICS AG WAGISTRASSE 27A PHONE +
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