Proceedings of the 10th International Colloquium on Paratuberculosis

More documents

Recommendations

Info

#145 MERKAL AWARD LECTURE Multinomial regression analysis <strong>of</strong> individual host factors and paratuberculosis test results Franziska Gierke, Mario Ziller, Heike Köhler Institute <strong>of</strong> Molecular Pathogenesis, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Germany; Institute <strong>of</strong> Epidemiology, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Germany Objective: Individual factors and paratuberculosis test results <strong>of</strong> infected cattle were integrated in a multinomial regression analysis to detect <strong>the</strong> most powerful animal influences on fecal and serological test results. Materials and Methods: 1.313 animals from 2 dairy cattle herds (A and B) were examined for fecal shedding <strong>of</strong> MAP by cultivation and confirmation <strong>of</strong> MAP by IS900-PCR as described before*. Culture results <strong>of</strong> infected animals were assigned to 3 groups: high, medium-low and low-no shedding. Blood and milk samples were tested with 2 paratuberculosis-ELISA-tests (nonabsorbed/LAM ELISA 1; absorbed ELISA 2). For <strong>the</strong> statistical analysis with <strong>the</strong> s<strong>of</strong>tware R (Version 2.4.0; http://www.r-project.org) test results and host factors were grouped in classes. Reference groups were selected randomly for <strong>the</strong> multinomial regression analysis. Results: Significant animal influences on <strong>the</strong> detection <strong>of</strong> high shedding were not established. The highest probability for medium-low shedding occurred during <strong>the</strong> 3rd lactation stage (211-315d). The most powerful influences for <strong>the</strong> detection <strong>of</strong> positive ELISA 1 blood or milk test results were animal age and a high intensity <strong>of</strong> shedding. A significantly higher chance for a positive ELISA 1 milk test result was detected in herd B. High daily milk yields (> 45 kg) were negatively associated with positive ELISA 1 milk test results. The occurrence <strong>of</strong> positive ELISA 2 blood and milk test results increased significantly when medium or high fecal shedding was detected simultaneously. ELISA 2 milk test results were less <strong>of</strong>ten positive when milk yields > 35 kg were recorded. Conclusion: High shedders can be detected irrespective <strong>of</strong> <strong>the</strong> examined individual factors. There is a high probability to detect medium-low fecal shedders during <strong>the</strong> 3rd lactation stage. Paratuberculosis-ELISAtest results were modified by individual host factors. Particularly <strong>the</strong> negative association <strong>of</strong> high milk yields and milk ELISA-test results should be taken into account when milk ELISA-tests are used for herd examinations. * Gierke, F., M. Ziller, H. Köhler (2007): Can <strong>the</strong> detection rate <strong>of</strong> faecal shedders <strong>of</strong> MAP be increased by optimising <strong>the</strong> time point <strong>of</strong> faecal sampling? Proc. 9ICP, Tsukuba, Japan, 107-110 149
#149 A meta-analysis <strong>of</strong> <strong>the</strong> effect <strong>of</strong> dose and age-at-exposure on shedding <strong>of</strong> Mycobacterium avium subspecies paratuberculosis (MAP) in experimentally infected calves and cows Rebecca Mans Mitchell, Graham F Medley, Michael T Collins, Ynte H Schukken College <strong>of</strong> Veterinary Medicine,Cornell University, USA; Department <strong>of</strong> Biological Sciences, University <strong>of</strong> Warwick, United Kingdom; Department <strong>of</strong> Pathobiological Sciences, School <strong>of</strong> Veterinary Medicine, University <strong>of</strong> Wisconsin, USA Calves artificially challenged with high doses <strong>of</strong> MAP bacteria have been reported to become infectious more quickly relative to low-dose challenge infections. However, <strong>the</strong> relationship between dose <strong>of</strong> challenge and speed <strong>of</strong> this progression has never been fully quantified. In ordinary differential equation (ODE)-based ma<strong>the</strong>matical models <strong>of</strong> infectious dynamics within herds, it is common to assume a constant rate <strong>of</strong> exit from each stage (compartment) <strong>of</strong> <strong>the</strong> infection process. Studies <strong>of</strong> properties <strong>of</strong> exit rate distributions indicated that <strong>the</strong> nature and heterogeneity <strong>of</strong> infection progression are highly influential on infection dynamics and may impact <strong>the</strong> efficacy <strong>of</strong> intervention strategies being evaluated. In this research, a meta-analysis was performed using all published and one unpublished long-term infection-challenge experiments to quantify <strong>the</strong> age- and dose-dependence <strong>of</strong> early and late shedding <strong>of</strong> Mycobacterium avium subsp. paratuberculosis (MAP). Studies were included if sufficient experimental details and sampling frequency were reported, resulting in 197 animals from 17 studies <strong>of</strong> which 176 were infected with a known dose and 21 were exposed naturally. Parametric time-to-event models indicated that challenging calves at a younger age and with a higher challenge dose resulted in faster transitions into shedding periods and longer durations <strong>of</strong> shedding. Experimentally infected calves shed during two distinct phases, with an Early Shedding stage within 12 months <strong>of</strong> infection, a Carrier period in which no bacteria can be cultured and a subsequent Late Shedding stage. In <strong>the</strong> current analysis, <strong>the</strong> possibility that animals do not experience Early Shedding but enter an Incubation period before Late Shedding is considered. The log-normal distribution was <strong>the</strong> most appropriate underlying distribution for infection-progression events. Calves that were exposed naturally showed variable progression rates, but not dissimilar to o<strong>the</strong>r infection routes. We propose that <strong>the</strong> observed infection patterns allow better understanding <strong>of</strong> low-grade endemicity <strong>of</strong> MAP in cattle. This work resulted in transition parameter estimates for future transmission dynamic modelling. 150
Page 1 and 2:
Book of Abstracts
Page 4 and 5:
Proceedings <stron
Page 6 and 7:
Grant Support and Sponsorship <stro
Page 8 and 9:
Scott Wells - Planning Committee Ch
Page 10 and 11:
Monday, August 10, 2009 - All sessi
Page 12 and 13:
Kari Røste Lybeck, Anne Kristine S
Page 14 and 15:
1120-1140 #88: Influence of
Page 16 and 17:
Concurrent Session B - Rm 175 Wille
Page 18 and 19:
Thursday, August 13 Thursday, Augus
Page 20:
Diagnostics and Genotyping Convenor
Page 23 and 24:
#229 Detecting Johne’s Disease he
Page 25 and 26:
Kalis CHJ, Hesselink JW, Barkema HW
Page 27 and 28:
MATERIALS AND METHODS Sampling For
Page 29 and 30:
To better determine the</st
Page 31 and 32:
#107 Multilocus Short Sequence Repe
Page 33 and 34:
Fig. 1. Dendogram (showing genetic
Page 35 and 36:
#105 MIRU-VNTR genotyping o
Page 37:
Diagnostics and Genotyping Poster A
Page 40 and 41:
Missouri). Serum from a cow with do
Page 42 and 43:
#7 Significance of
Page 44 and 45:
RESULTS Of the 327
Page 46 and 47:
Direct fecal nested Map PCR testing
Page 48 and 49:
#16 Histopathological and immunohis
Page 50 and 51:
#19 Development of
Page 52 and 53:
#36 Genetic diversity of</s
Page 54 and 55:
#60 Evaluation of
Page 56 and 57:
#66 Comparison of
Page 58 and 59:
(b) IS900 Nested PCR, using p90-p91
Page 60 and 61:
(d) f57 Real Time PCR. The figure o
Page 62 and 63:
• MIRU (3 loci); • VNTR-MIRU (7
Page 64 and 65:
indexes, compared to each single an
Page 66 and 67:
#108 Evaluation of
Page 68 and 69:
#110 Detection and molecular confir
Page 70 and 71:
endoscopic criteria and histopathol
Page 72 and 73:
Bull TJ, McMinn EJ, Sidi-Boumedine
Page 74 and 75:
#131 Seroprevalence of</str
Page 76 and 77:
could be partly attributed to a sma
Page 78 and 79:
#146 Analysis of v
Page 80 and 81:
#162 Comparison of
Page 82 and 83:
#187 Comparison of
Page 84 and 85:
#191 Potentiating day-old blood sam
Page 86 and 87:
samples from the s
Page 88 and 89:
(MAA) (MAP87 and MAP3776) and 1 fro
Page 90 and 91:
#196 Inter- and intra-subtype genot
Page 92 and 93:
RESULTS In 601 (29%) of</st
Page 94 and 95:
#211 Culture of my
Page 96 and 97:
#242 Application and field validati
Page 98 and 99:
#251 Diagnosis of
Page 100 and 101: Table 2. Paratuberculosis PCR resul
Page 102 and 103: #254 Programmes on Paratuberculosis
Page 104 and 105: Fig. 1. European countries specifyi
Page 106 and 107: A bulk milk quality assurance progr
Page 108 and 109: Republic of Lithua
Page 110 and 111: #276 Elimination of</strong
Page 112 and 113: other projects and
Page 114 and 115: Woodbine KA, Schukken YH, Green LE,
Page 116 and 117: Host Response and Immunology Keynot
Page 118 and 119: #33 MERKAL AWARD LECTURE No interfe
Page 120 and 121: #75 Interleukin 17 and interleukin
Page 122 and 123: #173 Local and systemic roles for b
Page 124 and 125: #23 Role of Mycoba
Page 126 and 127: Host Response and Immunology Poster
Page 128 and 129: #28 Intestinal MAP Infection via Pe
Page 130 and 131: #73 Age susceptibility of</
Page 132 and 133: #95 Role of nitric
Page 134 and 135: #134 Analysis of <
Page 136 and 137: #157 Study of <str
Page 138 and 139: #163 Immunohistochemical expression
Page 140 and 141: #193 Development of</strong
Page 142 and 143: #209 Murine macrophages carrying an
Page 144 and 145: #224 Application of</strong
Page 146 and 147: Table 1 UK and Cyprus MAP survey Re
Page 148 and 149: #228 Mycobacterium avium ssp. parat
Page 152 and 153: #50 Assessment of
Page 154 and 155: Table 1. Posterior median (CrIs) <s
Page 156 and 157: #55 Birth clusters of</stro
Page 158 and 159: from their dam, se
Page 160 and 161: #88 Influence of b
Page 162 and 163: #104 Relation between faecal shedde
Page 164 and 165: Epidemiology Poster Abstracts 109 1
Page 166 and 167: #22 The Prevalence of</stro
Page 168 and 169: CONCLUSION IS1311-based nested Ma/M
Page 170 and 171: #42 Seroprevalence survey o
Page 172 and 173: #77 Characterisation of</st
Page 174 and 175: Environmental samples were collecte
Page 176 and 177: REFERENCES 1) Chiodini RJ, Van Krui
Page 178 and 179: #113 Effect of soi
Page 180 and 181: #136 Error-adjusted, variance parti
Page 182 and 183: Latium Region Viterbo districts RES
Page 184 and 185: #144 The suitability of</st
Page 186 and 187: #178 Age structure of</stro
Page 188 and 189: A statistically significant lower f
Page 190 and 191: #183 Sero-prevalence of</st
Page 192 and 193: #218 Johne’s disease in t
Page 194 and 195: #244 A survey to estimate t
Page 196 and 197: Control Programs Keynote Lecture Re
Page 198 and 199: #202 Milk quality assurance for par
Page 200 and 201:
#141 Control of pa
Page 202 and 203:
#198 Use of small
Page 204 and 205:
Control Programs Poster Abstracts 1
Page 206 and 207:
RESULTS Loss of al
Page 208 and 209:
#40 Managing Bovine Johnes Disease
Page 210 and 211:
#74 Economic analysis of</s
Page 212 and 213:
#87 Evaluation of
Page 214 and 215:
separately for each existing herd <
Page 216 and 217:
#98 An inter-laboratory ring-trial
Page 218 and 219:
#120 Paratuberculosis vaccine inter
Page 220 and 221:
The statement provides: • A stand
Page 222 and 223:
COMPLEMENTARY ASPECTS On-Farm Disea
Page 224 and 225:
#168 Paratuberculosis in Iran: past
Page 226 and 227:
#180 Behavioural change in owners <
Page 228 and 229:
the fact that <str
Page 230 and 231:
#182 An integrated risk based appro
Page 232 and 233:
etween 0 to 10, depending on <stron
Page 234 and 235:
#203 Milk quality assurance for par
Page 236 and 237:
#234 Johne’s disease vaccine: a c
Page 238 and 239:
Pathogenomics and MAP Biology Conve
Page 240 and 241:
#45 Identification of</stro
Page 242 and 243:
#117 Adsorption of
Page 244 and 245:
#222 Atypical structural features <
Page 246 and 247:
Pathogenomics and MAP Biology Persp
Page 248 and 249:
247
Page 250 and 251:
#52 Is ‘Indian Bison Type’ Myco
Page 252 and 253:
#69 In vitro susceptibility <strong
Page 254 and 255:
#118 Cloning, expression and purifi
Page 256 and 257:
#176 A reference proteomic pr<stron
Page 258 and 259:
#213 Construction the</stro
Page 260 and 261:
#230 Iron concentration dependent s
Page 262 and 263:
261
Page 264 and 265:
263
Page 266 and 267:
Abstract not available at press tim
Page 268 and 269:
#174 Sharing of
Page 270 and 271:
#177 Associations between CARD15 po
Page 272 and 273:
Perspectives Talk: Public Health My
Page 274 and 275:
273
Page 276 and 277:
A total of 206 qua
Page 278 and 279:
Feller, M., K. Huwiler, R. Stephan,
Page 280 and 281:
In 2008, the Ameri
Page 282 and 283:
#115 Crohn’s disease and ruminant
Page 284 and 285:
International John
Page 286 and 287:
#76 Towards improved reporting stan
Page 288 and 289:
#97 The EU ParaTBTools Project - Th
Page 290 and 291:
#241 US Vaccine Initiative through
Page 292 and 293:
Paratuberculosis ELISA Reliable, si
Page 294 and 295:
PRIONICS AG WAGISTRASSE 27A PHONE +
show all

Proceedings of the 10th International Colloquium on Paratuberculosis

Create successful ePaper yourself

Delete template?

Save as template?