Proceedings of the 10th International Colloquium on Paratuberculosis

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#42 Seroprevalence survey <strong>of</strong> paratuberculosis in dairy and pastoral cattle in Uganda Julius Boniface Okuni, Lonzy Ojok Makerere University, Uganda Between 1999 and 2002, lesions consistent with <strong>the</strong> description <strong>of</strong> paratuberculosis were observed from abattoir specimens taken from slaughtered cattle. The aim <strong>of</strong> this study was to determine if <strong>the</strong>re was serological evidence for <strong>the</strong> prevalence <strong>of</strong> <strong>the</strong> disease among <strong>the</strong> cattle population. A total <strong>of</strong> 1023 cattle were selected by a multistage sampling technique from 66 cattle herds in 4 districts and tested for antibodies against M. paratuberculosis using Pourquier ELISA. The tested animals were from 7 different breeds, namely, Holstein Friesians, East African Zebu, Ankole long horn, Boran, Sahiwal, Jersey and Guernsey. At least 43 heads <strong>of</strong> cattle (4.2%) were serologically positive. 24 <strong>of</strong> <strong>the</strong> 66 herds <strong>of</strong> cattle had at least one positive animal (36%) based on <strong>the</strong> manufacturer’s cut <strong>of</strong>f. All <strong>the</strong> breeds <strong>of</strong> cattle except Guernsey had at least one infected animal. The disease was found to be present in all <strong>the</strong> four districts surveyed. Infection was also observed in cattle under all <strong>the</strong> different types <strong>of</strong> husbandry practices. This is <strong>the</strong> first time <strong>the</strong> prevalence <strong>of</strong> Johne’s disease has been determined in Uganda. Paratuberculosis has hi<strong>the</strong>rto been silently spreading among <strong>the</strong> cattle population in Uganda. This study provides information that will be used in future epidemiologic studies and control programs. #44 Genetic variation in serological response to Mycobacterium avium subspecies paratuberculosis and its association with performance in Irish Holstein-Friesian dairy cows Margaret Good, Peter Mullowney, Andrew Cromie, Simon J More Dept Agriculture, Fisheries and Food, Ireland; The Irish Cattle Breeding Federation, Ireland; Centre for Veterinary Epidemiology and Risk Analysis, Ireland Few estimates <strong>of</strong> <strong>the</strong> genetic variation in measures <strong>of</strong> susceptibility to MAP are available in <strong>the</strong> literature and even less have attempted to elucidate <strong>the</strong> genetic associations between measures <strong>of</strong> susceptibility to MAP and performance in dairy cattle. Therefore, <strong>the</strong> objectives <strong>of</strong> this study were to estimate <strong>the</strong> genetic variation in serological response to MAP in 5,280 Holstein-Friesian dairy cows, and to quantify its genetic association with performance traits measured in <strong>the</strong> first three lactations <strong>of</strong> genetically related animals. Univariate mixed linear and threshold animal models were used to estimate variance components and genetic correlations were estimated using bivariate sire linear mixed models; MAP serological response was treated as a continuous variable and dichotomous variable. The prevalence <strong>of</strong> MAP in <strong>the</strong> sample population was 4.8%. However, because <strong>of</strong> <strong>the</strong> data editing criteria used, this is not a representative statistic <strong>of</strong> <strong>the</strong> national prevalence. Estimates <strong>of</strong> heritability for MAP serological response varied from 0.07 to 0.15 depending on <strong>the</strong> model <strong>of</strong> analysis and whe<strong>the</strong>r serological response was treated as continuous or binary; standard errors varied from 0.024 and 0.062. Genetic correlations between MAP serological response and lactation milk, fat and protein yield were negative although not always more than two standard errors from zero; stronger negative genetic correlations were evident in older parity animals. Serological responseto MAP was not genetically correlated with milk fat concentration but was positively genetically correlated with lactation milk protein concentration and negatively correlated with calving interval. Positive genetic correlations existed between MAP serological response and somatic cell count but <strong>the</strong> correlations were not greater than two standard errors from zero. There was little or no genetic association between serological responseto MAP and survival. Results from this study corroborate previous international suggestions that selection for reduced serological responseto for MAP is possible, although this does not necessarily imply a concurrent selection for ei<strong>the</strong>r reduced prevalence <strong>of</strong> clinical disease or increased resistance to MAP infection. 169
#63 Risk <strong>of</strong> detecting Mycobacterium avium subsp. paratuberculosis (MAP) in colostrum and on teat skin <strong>of</strong> Holstein cows in Johne’s disease (JD) infected herds Patrick Pithua, Scott J Wells, Sandra M Godden Veterinary Population Medicine Department, College <strong>of</strong> Veterinary Medicine University <strong>of</strong> Minnesota, USA Objective: To describe <strong>the</strong> relationship between fecal shedding <strong>of</strong> MAP around calving time and detection <strong>of</strong> MAP in colostrum samples and on teat skin surfaces. Methods: Feces collected within 3 d prior to actual calving, colostrum and teat swabs collected immediately after calving from 112 cows in 4 JD endemic herds, were tested for MAP using culture and PCR targeting <strong>the</strong> gene ISMAP02 in colostrum and teat swabs. Logistic models were used to evaluate <strong>the</strong> relationship between MAP fecal shedding status and MAP detection in colostrum samples or teat swabs. Attributable fractions for <strong>the</strong> proportion <strong>of</strong> colostrum and teat swabs positive for MAP attributable to fecal shedding status <strong>of</strong> <strong>the</strong> donor cows were also calculated. Results: Odds ratio for detecting MAP in colostrum samples or teat swabs in fecal culture positive (vs. negative) cows were 2.02 (P < 0.001) and 1.87 (P < 0.008), respectively. Findings suggested that withholding colostrum from MAP fecal culture positive cows might reduce <strong>the</strong> risk <strong>of</strong> exposing calves to MAP through ingestion <strong>of</strong> colostrum by 18.2% and that limiting <strong>the</strong> chances <strong>of</strong> natural nursing by calves might reduce <strong>the</strong> risk <strong>of</strong> exposing calves to MAP present on teats <strong>of</strong> MAP fecal shedding dams by 19.5%. While <strong>the</strong> latter interventions might achieve some reduction in risk <strong>of</strong> exposure to MAP, <strong>the</strong>y by no means eliminate <strong>the</strong> risk <strong>of</strong> exposing calves to MAP through ingestion <strong>of</strong> MAP present in colostrum or on bovine teats in JD endemic herds since a greater proportion <strong>of</strong> MAP detected in <strong>the</strong> colostrum samples and teat swabs had sources unrelated to <strong>the</strong> fecal shedding status <strong>of</strong> <strong>the</strong> donors. Conclusion: Findings underscore need for adherence to practices that limit contact <strong>of</strong> calves with adult cows and to practices that promote hygienic colostrum handling to avoid possible contamination during harvest, storage or feeding processes. 170
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Book of Abstracts
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Proceedings <stron
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Grant Support and Sponsorship <stro
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Scott Wells - Planning Committee Ch
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Monday, August 10, 2009 - All sessi
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Kari Røste Lybeck, Anne Kristine S
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1120-1140 #88: Influence of
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Concurrent Session B - Rm 175 Wille
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Thursday, August 13 Thursday, Augus
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Diagnostics and Genotyping Convenor
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#229 Detecting Johne’s Disease he
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Kalis CHJ, Hesselink JW, Barkema HW
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MATERIALS AND METHODS Sampling For
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To better determine the</st
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#107 Multilocus Short Sequence Repe
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Fig. 1. Dendogram (showing genetic
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#105 MIRU-VNTR genotyping o
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Diagnostics and Genotyping Poster A
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Missouri). Serum from a cow with do
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#7 Significance of
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RESULTS Of the 327
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Direct fecal nested Map PCR testing
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#16 Histopathological and immunohis
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#19 Development of
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#36 Genetic diversity of</s
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#60 Evaluation of
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#66 Comparison of
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(b) IS900 Nested PCR, using p90-p91
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(d) f57 Real Time PCR. The figure o
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• MIRU (3 loci); • VNTR-MIRU (7
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indexes, compared to each single an
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#108 Evaluation of
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#110 Detection and molecular confir
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endoscopic criteria and histopathol
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Bull TJ, McMinn EJ, Sidi-Boumedine
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#131 Seroprevalence of</str
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could be partly attributed to a sma
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#146 Analysis of v
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#162 Comparison of
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#187 Comparison of
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#191 Potentiating day-old blood sam
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samples from the s
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(MAA) (MAP87 and MAP3776) and 1 fro
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#196 Inter- and intra-subtype genot
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RESULTS In 601 (29%) of</st
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#211 Culture of my
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#242 Application and field validati
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#251 Diagnosis of
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Table 2. Paratuberculosis PCR resul
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#254 Programmes on Paratuberculosis
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Fig. 1. European countries specifyi
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A bulk milk quality assurance progr
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Republic of Lithua
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#276 Elimination of</strong
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other projects and
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Woodbine KA, Schukken YH, Green LE,
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Host Response and Immunology Keynot
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#33 MERKAL AWARD LECTURE No interfe
Page 120 and 121: #75 Interleukin 17 and interleukin
Page 122 and 123: #173 Local and systemic roles for b
Page 124 and 125: #23 Role of Mycoba
Page 126 and 127: Host Response and Immunology Poster
Page 128 and 129: #28 Intestinal MAP Infection via Pe
Page 130 and 131: #73 Age susceptibility of</
Page 132 and 133: #95 Role of nitric
Page 134 and 135: #134 Analysis of <
Page 136 and 137: #157 Study of <str
Page 138 and 139: #163 Immunohistochemical expression
Page 140 and 141: #193 Development of</strong
Page 142 and 143: #209 Murine macrophages carrying an
Page 144 and 145: #224 Application of</strong
Page 146 and 147: Table 1 UK and Cyprus MAP survey Re
Page 148 and 149: #228 Mycobacterium avium ssp. parat
Page 150 and 151: #145 MERKAL AWARD LECTURE Multinomi
Page 152 and 153: #50 Assessment of
Page 154 and 155: Table 1. Posterior median (CrIs) <s
Page 156 and 157: #55 Birth clusters of</stro
Page 158 and 159: from their dam, se
Page 160 and 161: #88 Influence of b
Page 162 and 163: #104 Relation between faecal shedde
Page 164 and 165: Epidemiology Poster Abstracts 109 1
Page 166 and 167: #22 The Prevalence of</stro
Page 168 and 169: CONCLUSION IS1311-based nested Ma/M
Page 172 and 173: #77 Characterisation of</st
Page 174 and 175: Environmental samples were collecte
Page 176 and 177: REFERENCES 1) Chiodini RJ, Van Krui
Page 178 and 179: #113 Effect of soi
Page 180 and 181: #136 Error-adjusted, variance parti
Page 182 and 183: Latium Region Viterbo districts RES
Page 184 and 185: #144 The suitability of</st
Page 186 and 187: #178 Age structure of</stro
Page 188 and 189: A statistically significant lower f
Page 190 and 191: #183 Sero-prevalence of</st
Page 192 and 193: #218 Johne’s disease in t
Page 194 and 195: #244 A survey to estimate t
Page 196 and 197: Control Programs Keynote Lecture Re
Page 198 and 199: #202 Milk quality assurance for par
Page 200 and 201: #141 Control of pa
Page 202 and 203: #198 Use of small
Page 204 and 205: Control Programs Poster Abstracts 1
Page 206 and 207: RESULTS Loss of al
Page 208 and 209: #40 Managing Bovine Johnes Disease
Page 210 and 211: #74 Economic analysis of</s
Page 212 and 213: #87 Evaluation of
Page 214 and 215: separately for each existing herd <
Page 216 and 217: #98 An inter-laboratory ring-trial
Page 218 and 219: #120 Paratuberculosis vaccine inter
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The statement provides: • A stand
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COMPLEMENTARY ASPECTS On-Farm Disea
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#168 Paratuberculosis in Iran: past
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#180 Behavioural change in owners <
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the fact that <str
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#182 An integrated risk based appro
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etween 0 to 10, depending on <stron
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#203 Milk quality assurance for par
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#234 Johne’s disease vaccine: a c
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Pathogenomics and MAP Biology Conve
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#45 Identification of</stro
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#117 Adsorption of
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#222 Atypical structural features <
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Pathogenomics and MAP Biology Persp
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#52 Is ‘Indian Bison Type’ Myco
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#69 In vitro susceptibility <strong
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#118 Cloning, expression and purifi
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#176 A reference proteomic pr<stron
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#213 Construction the</stro
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#230 Iron concentration dependent s
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Abstract not available at press tim
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#174 Sharing of
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#177 Associations between CARD15 po
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Perspectives Talk: Public Health My
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A total of 206 qua
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Feller, M., K. Huwiler, R. Stephan,
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In 2008, the Ameri
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#115 Crohn’s disease and ruminant
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International John
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#76 Towards improved reporting stan
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#97 The EU ParaTBTools Project - Th
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#241 US Vaccine Initiative through
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Paratuberculosis ELISA Reliable, si
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PRIONICS AG WAGISTRASSE 27A PHONE +
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