Proceedings of the 10th International Colloquium on Paratuberculosis

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etween 0 to 10, depending on <strong>the</strong> herd’s BJD history, testing history and prevalence, geographical location (zone) and calf rearing practices. The higher <strong>the</strong> Score <strong>the</strong> greater is <strong>the</strong> assurance. Herds that do not test or implement calf rearing are rated below infected herds at a Score <strong>of</strong> 0. As hygienic calf rearing can significantly reduce <strong>the</strong> risk <strong>of</strong> calves becoming infected with bovine Johne’s disease, calves reared under <strong>the</strong> Victorian Johne’s Disease Calf Accreditation Program (JDCAP) are eligible for 3 extra points and those reared under an approved 3-Step Calf Rearing Plan are eligible for 1 extra point. Q-ALPACA After an initial outbreak in <strong>the</strong> early 1990s, Johne’s disease has been virtually eradicated from alpaca in Australia. However, alpaca <strong>of</strong>ten graze with o<strong>the</strong>r livestock and to help assure its members’ herds, <strong>the</strong> Australian Alpaca Association initiated this voluntary scheme with <strong>the</strong> endorsement <strong>of</strong> State and Federal animal health authorities in 2005. By requiring veterinary investigation <strong>of</strong> any death within a herd it not only monitors and assures for Johne’s disease, but also monitors and assists management <strong>of</strong> o<strong>the</strong>r significant endemic diseases, such as severe worm infestation, liver disease, liver fluke infestation and coccidiosis. GOAT RISK RATING The most recent scheme aims to improve understanding <strong>of</strong> goat health and reduce risks associated with Johne’s disease and a number <strong>of</strong> o<strong>the</strong>r important production diseases including footrot, caprine arthritis encephalitis (CAE) and lice, as well as drenching and vaccination history. Developed in 2008 by <strong>the</strong> Goat Industry Council <strong>of</strong> Australia (GICA) with state animal health authorities, it also promotes a simple message that higher <strong>the</strong> rating, <strong>the</strong> lower <strong>the</strong> risk. Like <strong>the</strong> sheep scheme, owners can voluntarily declare <strong>the</strong>ir herd’s Goat Risk Rating on <strong>the</strong> National Goat Health Statement CONCLUSION The implementation <strong>of</strong> risk based trading has largely ‘depoliticised’ <strong>the</strong> management <strong>of</strong> Johne’s disease and facilitated trade in south-eastern Australia where eradication <strong>of</strong> <strong>the</strong> disease is not considered possible in <strong>the</strong> foreseeable future. ADDITIONAL INFORMATION For additional information please refer to: Animal Health Australia http://www.animalhealthaustralia.com.au/programs/jd/jd_home.cfm Australian Alpaca Association http://www.alpaca.asn.au/pub/AAA/qa/docs/qa_faq2009.pdf Dairy Australia http://www.dairy.com.au/bjd 231
#185 Efficacy <strong>of</strong> ‘Indigenous vaccine’ in extended field trials conducted on <strong>the</strong> farm goa<strong>the</strong>rds naturally infected with Johne’s disease Shoor Vir Singh, Pravin Kumar Singh, Ajay Vir Singh, Jagdeep Singh Sohal, M C Sharma,Central Institute for Research on Goats, India Objective: Extended field trials were conducted to know <strong>the</strong> efficacy <strong>of</strong> ‘Indigenous Johne’s disease (JD) vaccine’ in goa<strong>the</strong>rds naturally infected with MAP (<strong>the</strong>rapeutic effect). Materials and Methods: ‘Indigenous vaccine’ (extended field trials) was evaluated in two herds <strong>of</strong> goats naturally infected with Johne’s disease, consisting <strong>of</strong> tall breeds from south- western India located at CIRG, Makhdoom. Goats were vaccinated with ‘indigenous vaccine’, after failing to contain MAP infection by traditional test and cull method for past 25 years. One herd consisted <strong>of</strong> 219 existing goats <strong>of</strong> Sirohi, Jamunapari, Marwari, Barbari and local non-descript breed and newly purchased 66 goats <strong>of</strong> Sirhoi and Barbari breeds were vaccinated. Ano<strong>the</strong>r herd consisting <strong>of</strong> 228 goats was <strong>of</strong> Jakharan breed <strong>of</strong> nor<strong>the</strong>rn Rajasthan and was endemic for JD. Existing parameters in-terms <strong>of</strong> health status and productivity were control. Serum and fecal samples <strong>of</strong> vaccinated goats were collected before and after vaccination and screened by Indigenous ELISA kit’ and microscopy. Goats were monitored for improvements in physical conditions, infection status, mortality, morbidity (diarrhea, etc.), production (growth rate, milk yield) and reproduction performances (twining percent), kid survivability, ELISA titers, fecal shedding <strong>of</strong> MAP. Inactivated JD vaccine’ was prepared from native, ‘Indian Bison Type’ Mycobacterium avium paratuberculosis (MAP) strain <strong>of</strong> goat origin. Results and Conclusions: There was visible improvement in <strong>the</strong> physical condition <strong>of</strong> vaccinated goats. Goats regained luster and shine <strong>of</strong> body coat, were alert, active, glistening eyes etc. Sero-conversion with long term increased titer was observed in vaccinated goats. Number <strong>of</strong> MAP shedders decreased with marked overall improvement in body conditions <strong>of</strong> vaccinated goats. Average gain in body weights recorded constant improvement. Percent survivability, birth weight and growth rate was higher especially in young kids. There was significant improvement in twining percent (60.0%) and milk yield (90 day, 120 days and total milk yield). Morbidity and mortality was reduced significantly. Improvement was more in newly purchased goats as compared to existing goats at <strong>the</strong> farm. ‘Indigenous vaccine’ showed ‘Therapeutic effect’ in goa<strong>the</strong>rds endemically infected with JD. 232
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Book of Abstracts
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Proceedings <stron
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Grant Support and Sponsorship <stro
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Scott Wells - Planning Committee Ch
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Monday, August 10, 2009 - All sessi
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Kari Røste Lybeck, Anne Kristine S
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1120-1140 #88: Influence of
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Concurrent Session B - Rm 175 Wille
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Thursday, August 13 Thursday, Augus
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Diagnostics and Genotyping Convenor
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#229 Detecting Johne’s Disease he
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Kalis CHJ, Hesselink JW, Barkema HW
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MATERIALS AND METHODS Sampling For
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To better determine the</st
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#107 Multilocus Short Sequence Repe
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Fig. 1. Dendogram (showing genetic
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#105 MIRU-VNTR genotyping o
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Diagnostics and Genotyping Poster A
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Missouri). Serum from a cow with do
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#7 Significance of
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RESULTS Of the 327
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Direct fecal nested Map PCR testing
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#16 Histopathological and immunohis
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#19 Development of
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#36 Genetic diversity of</s
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#60 Evaluation of
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#66 Comparison of
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(b) IS900 Nested PCR, using p90-p91
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(d) f57 Real Time PCR. The figure o
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• MIRU (3 loci); • VNTR-MIRU (7
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indexes, compared to each single an
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#108 Evaluation of
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#110 Detection and molecular confir
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endoscopic criteria and histopathol
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Bull TJ, McMinn EJ, Sidi-Boumedine
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#131 Seroprevalence of</str
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could be partly attributed to a sma
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#146 Analysis of v
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#162 Comparison of
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#187 Comparison of
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#191 Potentiating day-old blood sam
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samples from the s
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(MAA) (MAP87 and MAP3776) and 1 fro
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#196 Inter- and intra-subtype genot
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RESULTS In 601 (29%) of</st
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#211 Culture of my
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#242 Application and field validati
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#251 Diagnosis of
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Table 2. Paratuberculosis PCR resul
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#254 Programmes on Paratuberculosis
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Fig. 1. European countries specifyi
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A bulk milk quality assurance progr
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Republic of Lithua
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#276 Elimination of</strong
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other projects and
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Woodbine KA, Schukken YH, Green LE,
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Host Response and Immunology Keynot
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#33 MERKAL AWARD LECTURE No interfe
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#75 Interleukin 17 and interleukin
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#173 Local and systemic roles for b
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#23 Role of Mycoba
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Host Response and Immunology Poster
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#28 Intestinal MAP Infection via Pe
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#73 Age susceptibility of</
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#95 Role of nitric
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#134 Analysis of <
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#157 Study of <str
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#163 Immunohistochemical expression
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#193 Development of</strong
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#209 Murine macrophages carrying an
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#224 Application of</strong
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Table 1 UK and Cyprus MAP survey Re
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#228 Mycobacterium avium ssp. parat
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#145 MERKAL AWARD LECTURE Multinomi
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#50 Assessment of
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Table 1. Posterior median (CrIs) <s
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#55 Birth clusters of</stro
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from their dam, se
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#88 Influence of b
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#104 Relation between faecal shedde
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Epidemiology Poster Abstracts 109 1
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#22 The Prevalence of</stro
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CONCLUSION IS1311-based nested Ma/M
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#42 Seroprevalence survey o
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#77 Characterisation of</st
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Environmental samples were collecte
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REFERENCES 1) Chiodini RJ, Van Krui
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#113 Effect of soi
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#136 Error-adjusted, variance parti
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Page 188 and 189: A statistically significant lower f
Page 190 and 191: #183 Sero-prevalence of</st
Page 192 and 193: #218 Johne’s disease in t
Page 194 and 195: #244 A survey to estimate t
Page 196 and 197: Control Programs Keynote Lecture Re
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Page 200 and 201: #141 Control of pa
Page 202 and 203: #198 Use of small
Page 204 and 205: Control Programs Poster Abstracts 1
Page 206 and 207: RESULTS Loss of al
Page 208 and 209: #40 Managing Bovine Johnes Disease
Page 210 and 211: #74 Economic analysis of</s
Page 212 and 213: #87 Evaluation of
Page 214 and 215: separately for each existing herd <
Page 216 and 217: #98 An inter-laboratory ring-trial
Page 218 and 219: #120 Paratuberculosis vaccine inter
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Page 238 and 239: Pathogenomics and MAP Biology Conve
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#115 Crohn’s disease and ruminant
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International John
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#76 Towards improved reporting stan
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#97 The EU ParaTBTools Project - Th
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#241 US Vaccine Initiative through
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Paratuberculosis ELISA Reliable, si
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PRIONICS AG WAGISTRASSE 27A PHONE +
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