Proceedings of the 10th International Colloquium on Paratuberculosis

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#183 Sero-prevalence <strong>of</strong> Mycobacterium avium subspecies paratuberculosis infection in low productive domestic ruminants with clinical and sub-clinical Johne’s disease in India Shoor Vir Singh, Ajay Vir SIngh, Pravin Kumar Singh, Jagdeep Singh Sohal, M C Sharma Central Institute for Research on Goats, India Objective: Status <strong>of</strong> Mycobacterium avium subspecies paratuberculosis (MAP) infection was estimated in domestic ruminants’ in India using ‘indigenous ELISA kit’. Methods: Indirect ELISA (un-absorbed) using protoplasmic antigen from ‘Indian Bison Type’ MAP <strong>of</strong> goat origin was used to screen all species <strong>of</strong> domestic ruminants. OD values were converted to S/P ratios to know status <strong>of</strong> Johne’s disease (JD) as negatives, suspected, low positive, positive and strong positives. Animals in strong positives were considered positive for JD. Sero-prevalence was estimated on <strong>the</strong> basis <strong>of</strong> screening <strong>of</strong> serum samples submitted to Microbiology laboratory <strong>of</strong> Central Institute for Research on Goats, Makhdoom, over period <strong>of</strong> 4 years (2004 – 2008), in-view <strong>of</strong> lack <strong>of</strong> diagnostic kits against JD in <strong>the</strong> country. Total <strong>of</strong> 2478 serum (1289 cows, 515 buffaloes, 536 goats and 138 sheep) were screened in 4 years and samples were driven from Punjab, Hayana, Uttar Pradesh, Tamil Nadu and Rajasthan states <strong>of</strong> India.. Results and Conclusions: Of 2478 samples sero-prevalence <strong>of</strong> MAP was 22.5% in domestic ruminants in 5 states <strong>of</strong> India. Species-wise sero-prevalence was 23.3, 21.3, 23.5 and 17.4% in cattle, buffaloes, goats and sheep, respectively. Distribution <strong>of</strong> 2478 serum samples with respect to <strong>the</strong> status <strong>of</strong> JD was 20.4, 6.4, 8.5, 42.0 and 22.5% as negatives, suspected, low positives, positives and strong positivess, respectively. Comparison <strong>of</strong> ELISA kit with fecal culture, show maximum correlation with strong positive category, however, fecal culture detected positive animals from all categories <strong>of</strong> S/P ratio. Samples in <strong>the</strong> positive category also exhibited substantial correlation with culture, <strong>the</strong>refore if positive and strong positive were considered positive, <strong>the</strong>n seroprevalence <strong>of</strong> MAP infection in domestic ruminants in India was 64.5%. Low per animal productivity substantiated this high sero-prevalence <strong>of</strong> MAP infection in domestic ruminants <strong>of</strong> country. Still considering culture being positive in o<strong>the</strong>r categories also, <strong>the</strong>se figures may still be under statement. Sero-prevalence <strong>of</strong> MAP infection in <strong>the</strong> domestic ruminants on <strong>the</strong> basis <strong>of</strong> screening <strong>of</strong> 2478 animals over period <strong>of</strong> 4 years (2004-08) from <strong>the</strong> 5 states <strong>of</strong> India was 22.5%. 189
#201 Prevalence and distribution <strong>of</strong> paratuberculosis (Johne’s Disease) in cattle herds in Ireland Margaret Good, Tracy Clegg, Hazel Sheridan, Dermot Yearsley, Tony O’Brien, John Egan, Peter Mullowney Dept Agriculture, Fisheries and Food, Ireland; Centre for Veterinary Epidemiology and Risk Analysis, University College Dublin, Ireland A simple random survey was conducted in Ireland during 2005 to estimate <strong>the</strong> seroprevalence <strong>of</strong> paratuberculosis, commonly called Johne’s disease (JD), in <strong>the</strong> cattle population. Serum samples were collected from all 20,322 females/breeding bulls over twelve months <strong>of</strong> age in 639 herds (0.7% <strong>of</strong> breeding herds). All samples were tested using a commercially available absorbed ELISA. The overall prevalence <strong>of</strong> seropositive herds was 21.4% (95% CI = 18.4%, 24.9%). Herd prevalence levels amongst dairy herds (mean 31.5%; CI 24.6%-39.3%) was higher than among beef herds (mean 17.9 %; CI 14.6%-21.8%). However, <strong>the</strong> animal level prevalence was similar. The majority <strong>of</strong> herds had only one seropositve animal. Only 6.4% (95% CI 4.7%-8.7%) <strong>of</strong> all herds had more than one seropositive animal; 13.3% (CI 8.7%-19.7%) <strong>of</strong> dairy herds ranging from two to eight seropositive animals and 3.9% beef herds (CI 2.4%-6.2%) ranging from two to five seropositive animals. The true prevalence <strong>of</strong> herds infected and shedding Mycobacterium avium subspecies paratuberculosis is estimated to be 9.51% for all herd types; 20.63% for dairy herds and 7.58% for beef herds based on a test specificity <strong>of</strong> 99.8% and test sensitivity (i.e. ability to detect culture-positive animals shedding at any level) <strong>of</strong> 27.8-28.9%. #216 Calf shedding <strong>of</strong> MAP on two farms with high and low environmental exposure Rebecca Mans Mitchell, Robert H Whitlock, Julie M Smith, Michael Wood, Nicole S Gollnick, Ynte H Schukken College <strong>of</strong> Veterinary Medicine, Cornell University, USA; New Bolton Center, University <strong>of</strong> Pennsylvania, USA; University <strong>of</strong> Vermont, USA; Vermont Agency <strong>of</strong> Agriculture, Food and Markets, USA; Clinic for Ruminants, University <strong>of</strong> Munich, Germany Two cohorts <strong>of</strong> calves were followed for 11 months by monthly fecal culture on two nor<strong>the</strong>astern USA dairy farms with known MAP prevalence. Index calves were selected from dams classified as moderate or high shedders based on fecal culture. The remainder <strong>of</strong> <strong>the</strong> cohort was selected to be most likely to be raised in <strong>the</strong> same environment as <strong>the</strong> index animal. On both farms, fecal samples were collected monthly and stored until completion <strong>of</strong> <strong>the</strong> study. Samples were batch processed to decrease variability between timepoints. Samples were evaluated at New Bolton Center by Tetracore’s Vet-AlertTM real-time PCR and fecal culture <strong>of</strong> those samples with positive PCRs. On farm A, no positive samples from animals were identified including <strong>the</strong> index animal. On farm B, 4 animals in <strong>the</strong> cohort had positive (Ct≤42) or suspicious (Ct> 42) PCRs (a total <strong>of</strong> 7 potential positive samples were identified out <strong>of</strong> 146). The index animal was negative for all cultures. Calves in farm B were housed in <strong>the</strong> same barns as <strong>the</strong> milking herd for 6 months before transitioning to heifer barns. Environmental samples were ga<strong>the</strong>red from within calf pens and <strong>the</strong> contiguous milking herd pens to evaluate <strong>the</strong> likelihood <strong>of</strong> post-birth exposure. Environmental samples were positive by RT-PCR at 8 <strong>of</strong> 11 sampling timepoints, and for 4 <strong>of</strong> <strong>the</strong> 6 timepoints in which calves were housed exclusively with o<strong>the</strong>r calves. Although this analysis did not reveal positive cultures from <strong>the</strong> index animal, on farm B <strong>the</strong>re were multiple potentially positive calves in <strong>the</strong> cohort, one <strong>of</strong> which cultured positive 3 times in <strong>the</strong> course <strong>of</strong> 4 months. While we cannot illustrate calf-to-calf transmission, we do see culture positive young animals, even when housed in an environment which should not involve substantial exposure from adult animals. 190
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Book of Abstracts
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Proceedings <stron
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Grant Support and Sponsorship <stro
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Scott Wells - Planning Committee Ch
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Monday, August 10, 2009 - All sessi
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Kari Røste Lybeck, Anne Kristine S
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1120-1140 #88: Influence of
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Concurrent Session B - Rm 175 Wille
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Thursday, August 13 Thursday, Augus
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Diagnostics and Genotyping Convenor
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#229 Detecting Johne’s Disease he
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Kalis CHJ, Hesselink JW, Barkema HW
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MATERIALS AND METHODS Sampling For
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To better determine the</st
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#107 Multilocus Short Sequence Repe
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Fig. 1. Dendogram (showing genetic
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#105 MIRU-VNTR genotyping o
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Diagnostics and Genotyping Poster A
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Missouri). Serum from a cow with do
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#7 Significance of
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RESULTS Of the 327
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Direct fecal nested Map PCR testing
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#16 Histopathological and immunohis
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#19 Development of
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#36 Genetic diversity of</s
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#60 Evaluation of
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#66 Comparison of
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(b) IS900 Nested PCR, using p90-p91
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(d) f57 Real Time PCR. The figure o
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• MIRU (3 loci); • VNTR-MIRU (7
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indexes, compared to each single an
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#108 Evaluation of
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#110 Detection and molecular confir
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endoscopic criteria and histopathol
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Bull TJ, McMinn EJ, Sidi-Boumedine
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#131 Seroprevalence of</str
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could be partly attributed to a sma
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#146 Analysis of v
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#162 Comparison of
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#187 Comparison of
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#191 Potentiating day-old blood sam
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samples from the s
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(MAA) (MAP87 and MAP3776) and 1 fro
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#196 Inter- and intra-subtype genot
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RESULTS In 601 (29%) of</st
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#211 Culture of my
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#242 Application and field validati
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#251 Diagnosis of
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Table 2. Paratuberculosis PCR resul
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#254 Programmes on Paratuberculosis
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Fig. 1. European countries specifyi
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A bulk milk quality assurance progr
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Republic of Lithua
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#276 Elimination of</strong
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other projects and
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Woodbine KA, Schukken YH, Green LE,
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Host Response and Immunology Keynot
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#33 MERKAL AWARD LECTURE No interfe
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#75 Interleukin 17 and interleukin
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#173 Local and systemic roles for b
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#23 Role of Mycoba
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Host Response and Immunology Poster
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#28 Intestinal MAP Infection via Pe
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#73 Age susceptibility of</
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#95 Role of nitric
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#134 Analysis of <
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#157 Study of <str
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#163 Immunohistochemical expression
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Page 142 and 143: #209 Murine macrophages carrying an
Page 144 and 145: #224 Application of</strong
Page 146 and 147: Table 1 UK and Cyprus MAP survey Re
Page 148 and 149: #228 Mycobacterium avium ssp. parat
Page 150 and 151: #145 MERKAL AWARD LECTURE Multinomi
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Page 154 and 155: Table 1. Posterior median (CrIs) <s
Page 156 and 157: #55 Birth clusters of</stro
Page 158 and 159: from their dam, se
Page 160 and 161: #88 Influence of b
Page 162 and 163: #104 Relation between faecal shedde
Page 164 and 165: Epidemiology Poster Abstracts 109 1
Page 166 and 167: #22 The Prevalence of</stro
Page 168 and 169: CONCLUSION IS1311-based nested Ma/M
Page 170 and 171: #42 Seroprevalence survey o
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Page 174 and 175: Environmental samples were collecte
Page 176 and 177: REFERENCES 1) Chiodini RJ, Van Krui
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Page 180 and 181: #136 Error-adjusted, variance parti
Page 182 and 183: Latium Region Viterbo districts RES
Page 184 and 185: #144 The suitability of</st
Page 186 and 187: #178 Age structure of</stro
Page 188 and 189: A statistically significant lower f
Page 192 and 193: #218 Johne’s disease in t
Page 194 and 195: #244 A survey to estimate t
Page 196 and 197: Control Programs Keynote Lecture Re
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Page 200 and 201: #141 Control of pa
Page 202 and 203: #198 Use of small
Page 204 and 205: Control Programs Poster Abstracts 1
Page 206 and 207: RESULTS Loss of al
Page 208 and 209: #40 Managing Bovine Johnes Disease
Page 210 and 211: #74 Economic analysis of</s
Page 212 and 213: #87 Evaluation of
Page 214 and 215: separately for each existing herd <
Page 216 and 217: #98 An inter-laboratory ring-trial
Page 218 and 219: #120 Paratuberculosis vaccine inter
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Page 222 and 223: COMPLEMENTARY ASPECTS On-Farm Disea
Page 224 and 225: #168 Paratuberculosis in Iran: past
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Page 238 and 239: Pathogenomics and MAP Biology Conve
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#45 Identification of</stro
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#117 Adsorption of
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#222 Atypical structural features <
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Pathogenomics and MAP Biology Persp
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#52 Is ‘Indian Bison Type’ Myco
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#69 In vitro susceptibility <strong
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#118 Cloning, expression and purifi
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#176 A reference proteomic pr<stron
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#213 Construction the</stro
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#230 Iron concentration dependent s
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Abstract not available at press tim
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#174 Sharing of
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#177 Associations between CARD15 po
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Perspectives Talk: Public Health My
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A total of 206 qua
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Feller, M., K. Huwiler, R. Stephan,
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In 2008, the Ameri
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#115 Crohn’s disease and ruminant
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International John
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#76 Towards improved reporting stan
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#97 The EU ParaTBTools Project - Th
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#241 US Vaccine Initiative through
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Paratuberculosis ELISA Reliable, si
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PRIONICS AG WAGISTRASSE 27A PHONE +
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Proceedings of the 10th International Colloquium on Paratuberculosis

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