Proceedings of the 10th International Colloquium on Paratuberculosis

More documents

Recommendations

Info

#234 Johne’s disease vaccine: a cohort study measuring long-term effectiveness <strong>of</strong> <strong>the</strong> whole cell killed bacterin Barbara Knust, Darlene Konkle, Jeffery Bohn, Scott Wells, Elisabeth Patton, University <strong>of</strong> Minnesota, USA; Wisconsin Department <strong>of</strong> Agriculture, Trade and Consumer Protection, USA In this prospective cohort study, three commercial dairy herds vaccinated every o<strong>the</strong>r heifer calf using a whole cell killed Johne’s vaccine until two cohorts were obtained. O<strong>the</strong>r Johne’s disease management efforts on <strong>the</strong> farms were used, including annual risk assessments, but <strong>the</strong> management <strong>of</strong> vaccinates and controls was <strong>the</strong> same in <strong>the</strong> herd. Fecal samples from heifers from <strong>the</strong> cohort groups were collected at first calving and at 90 days <strong>of</strong> pregnancy at each subsequent lactation and tested using bacterial culture with liquid media. Fecal samples from <strong>the</strong> rest <strong>of</strong> <strong>the</strong> herd were also collected and cultured annually at 90 days <strong>of</strong> pregnancy. Herd production data was collected on a semiannual basis, and data regarding culling and reasons for culling was recorded. Baseline prevalence estimates indicated <strong>the</strong> herds were moderately to heavily infected with Johne’s disease at <strong>the</strong> start <strong>of</strong> <strong>the</strong> study. Five years after <strong>the</strong> initial cohort animals were vaccinated, significantly fewer vaccinates were culled due to clinical Johne’s disease, and vaccinates had a significantly lower level <strong>of</strong> fecal shedding. Survival analysis found vaccinates survived a significantly longer time until being culled for clinical disease, and had a longer time to first positive test result than controls. Vaccination however did not have an effect on <strong>the</strong> overall survival in <strong>the</strong> herd if culling for any reason was measured. Whole herd fecal shedding prevalence decreased on all three farms from <strong>the</strong> beginning <strong>of</strong> <strong>the</strong> study. The results <strong>of</strong> this study demonstrate that use <strong>of</strong> <strong>the</strong> commercially available Johne’s disease vaccine serves as a valuable tool in reduction <strong>of</strong> clinical signs and eventual disease eradication. #236 Producer’s perception <strong>of</strong> a voluntary Johne’s disease control program in Ontario and Western Canada Ulrike Sorge, David Kelton, Kerry Lissemore, Ann Godkin, Steve Henrick, Scott Wells, University <strong>of</strong> Guelph, Canada; Ontario Ministry <strong>of</strong> Agriculture, Food and Rural Affairs, Canada; University <strong>of</strong> Saskatchewan, Canada; University <strong>of</strong> Minnesota, USA Producer’s compliance with recommended management practices is crucial for <strong>the</strong> success <strong>of</strong> Risk Assessment (RA) based Johne’s disease (JD) control programs. The objective <strong>of</strong> this study was to assess dairy producers’ perception <strong>of</strong> JD and a JD RA based control program. A voluntary RA based control program for JD was initiated in Ontario and Western Canada in 2005-07. A subset <strong>of</strong> 238 producers were contacted through a telephone survey in 2008 asking about <strong>the</strong>ir perception <strong>of</strong> <strong>the</strong> importance <strong>of</strong> JD, <strong>the</strong> program and costs for implementation <strong>of</strong> farm-specific recommendations as well as <strong>the</strong>ir reasons against implementing suggestions. In 2005-07 most farms were test-negative and in 2008 most producers did not see JD as a problem for <strong>the</strong>ir farm. Producers indicated that <strong>the</strong>y were participating in <strong>the</strong> program, because <strong>the</strong>y saw <strong>the</strong> economic impact <strong>of</strong> <strong>the</strong> disease, wanted to be proactive or because <strong>the</strong>y were concerned that <strong>the</strong> consumer’s perception <strong>of</strong> an association between MAP and Crohn’s disease could affect <strong>the</strong> dairy industry. The program and <strong>the</strong> farm-specific recommendations were generally well received. The majority <strong>of</strong> producers had implemented at least one recommendation. However, on average only 2 out <strong>of</strong> 6 suggestions were implemented. The recommendation that had <strong>the</strong> highest compliance was culling <strong>of</strong> test-positive cows. This was also <strong>the</strong> main reason why producers decided against fur<strong>the</strong>r changes. After <strong>the</strong>y culled <strong>the</strong> test-positive cow, <strong>the</strong>y did not see <strong>the</strong> necessity for additional changes. Most producers were unable to quantify <strong>the</strong> costs associated with management changes, but felt that some changes actually saved time and money. In addition, many producers reported better herd health or less calf disease after implementation <strong>of</strong> at least one recommendation. In conclusion, <strong>the</strong>se results indicate that <strong>the</strong>se recommendations can lead to improved herd health which may be used to convince producers to implement recommended management changes. 235
#240 A survey <strong>of</strong> dairy producers in <strong>the</strong> United States concerning Johne’s program participation Ernest Hovingh, Kenneth Olson Penn State University, USA; KEO Consulting, USA The United States Department <strong>of</strong> Agriculture has promoted a voluntary Bovine Johne’s Disease control program with funds being made available to states to support program implementation. The number <strong>of</strong> enrolled herds showed evidence <strong>of</strong> plateauing and information was desired about <strong>the</strong> reasons that producers did or did not participate in <strong>the</strong> program, and <strong>the</strong> perceived value <strong>of</strong> <strong>the</strong> program. A survey was developed and mailed to a systematic random sample (N=8,013) <strong>of</strong> all USA dairy herds. Additional mailings were used to increase <strong>the</strong> return rate, and 2601 surveys were returned. Herd size distribution <strong>of</strong> respondents was consistent with o<strong>the</strong>r national data. Herds participating in <strong>the</strong>ir state’s JD program (27%) were slightly over-represented in <strong>the</strong> dataset. 48% <strong>of</strong> <strong>the</strong> respondents “agreed” or “strongly agreed” that JD was currently a concern in <strong>the</strong>ir herd, and 55% indicated <strong>the</strong>y expected it to be a future concern. Logistic regression modeling was used to determine <strong>the</strong> factors associated with <strong>the</strong> probability <strong>of</strong> participation in <strong>the</strong> state Johne’s program. Regional, herd size, disease knowledge and motivational factors were among those evaluated. 24% <strong>of</strong> responders that were not participating in <strong>the</strong> state program felt that <strong>the</strong>y were already doing everything <strong>the</strong>y could to manage JD, such that program participation was perceived to provide no additional benefit. The respondents appeared to have a reasonable level <strong>of</strong> knowledge <strong>of</strong> <strong>the</strong> transmission <strong>of</strong> JD, but misunderstanding about <strong>the</strong> interpretation and use <strong>of</strong> diagnostic test results was evident in <strong>the</strong> results. Questions were asked to determine <strong>the</strong> economic importance <strong>of</strong> JD. 74% <strong>of</strong> <strong>the</strong> respondents indicated that <strong>the</strong>y would be willing to pay a premium for replacement animals that had at least a 95% probability <strong>of</strong> being free <strong>of</strong> JD. The results <strong>of</strong> this study will be useful to guide Johne’s education and control program implementation efforts. 236
Page 1 and 2:
Book of Abstracts
Page 4 and 5:
Proceedings <stron
Page 6 and 7:
Grant Support and Sponsorship <stro
Page 8 and 9:
Scott Wells - Planning Committee Ch
Page 10 and 11:
Monday, August 10, 2009 - All sessi
Page 12 and 13:
Kari Røste Lybeck, Anne Kristine S
Page 14 and 15:
1120-1140 #88: Influence of
Page 16 and 17:
Concurrent Session B - Rm 175 Wille
Page 18 and 19:
Thursday, August 13 Thursday, Augus
Page 20:
Diagnostics and Genotyping Convenor
Page 23 and 24:
#229 Detecting Johne’s Disease he
Page 25 and 26:
Kalis CHJ, Hesselink JW, Barkema HW
Page 27 and 28:
MATERIALS AND METHODS Sampling For
Page 29 and 30:
To better determine the</st
Page 31 and 32:
#107 Multilocus Short Sequence Repe
Page 33 and 34:
Fig. 1. Dendogram (showing genetic
Page 35 and 36:
#105 MIRU-VNTR genotyping o
Page 37:
Diagnostics and Genotyping Poster A
Page 40 and 41:
Missouri). Serum from a cow with do
Page 42 and 43:
#7 Significance of
Page 44 and 45:
RESULTS Of the 327
Page 46 and 47:
Direct fecal nested Map PCR testing
Page 48 and 49:
#16 Histopathological and immunohis
Page 50 and 51:
#19 Development of
Page 52 and 53:
#36 Genetic diversity of</s
Page 54 and 55:
#60 Evaluation of
Page 56 and 57:
#66 Comparison of
Page 58 and 59:
(b) IS900 Nested PCR, using p90-p91
Page 60 and 61:
(d) f57 Real Time PCR. The figure o
Page 62 and 63:
• MIRU (3 loci); • VNTR-MIRU (7
Page 64 and 65:
indexes, compared to each single an
Page 66 and 67:
#108 Evaluation of
Page 68 and 69:
#110 Detection and molecular confir
Page 70 and 71:
endoscopic criteria and histopathol
Page 72 and 73:
Bull TJ, McMinn EJ, Sidi-Boumedine
Page 74 and 75:
#131 Seroprevalence of</str
Page 76 and 77:
could be partly attributed to a sma
Page 78 and 79:
#146 Analysis of v
Page 80 and 81:
#162 Comparison of
Page 82 and 83:
#187 Comparison of
Page 84 and 85:
#191 Potentiating day-old blood sam
Page 86 and 87:
samples from the s
Page 88 and 89:
(MAA) (MAP87 and MAP3776) and 1 fro
Page 90 and 91:
#196 Inter- and intra-subtype genot
Page 92 and 93:
RESULTS In 601 (29%) of</st
Page 94 and 95:
#211 Culture of my
Page 96 and 97:
#242 Application and field validati
Page 98 and 99:
#251 Diagnosis of
Page 100 and 101:
Table 2. Paratuberculosis PCR resul
Page 102 and 103:
#254 Programmes on Paratuberculosis
Page 104 and 105:
Fig. 1. European countries specifyi
Page 106 and 107:
A bulk milk quality assurance progr
Page 108 and 109:
Republic of Lithua
Page 110 and 111:
#276 Elimination of</strong
Page 112 and 113:
other projects and
Page 114 and 115:
Woodbine KA, Schukken YH, Green LE,
Page 116 and 117:
Host Response and Immunology Keynot
Page 118 and 119:
#33 MERKAL AWARD LECTURE No interfe
Page 120 and 121:
#75 Interleukin 17 and interleukin
Page 122 and 123:
#173 Local and systemic roles for b
Page 124 and 125:
#23 Role of Mycoba
Page 126 and 127:
Host Response and Immunology Poster
Page 128 and 129:
#28 Intestinal MAP Infection via Pe
Page 130 and 131:
#73 Age susceptibility of</
Page 132 and 133:
#95 Role of nitric
Page 134 and 135:
#134 Analysis of <
Page 136 and 137:
#157 Study of <str
Page 138 and 139:
#163 Immunohistochemical expression
Page 140 and 141:
#193 Development of</strong
Page 142 and 143:
#209 Murine macrophages carrying an
Page 144 and 145:
#224 Application of</strong
Page 146 and 147:
Table 1 UK and Cyprus MAP survey Re
Page 148 and 149:
#228 Mycobacterium avium ssp. parat
Page 150 and 151:
#145 MERKAL AWARD LECTURE Multinomi
Page 152 and 153:
#50 Assessment of
Page 154 and 155:
Table 1. Posterior median (CrIs) <s
Page 156 and 157:
#55 Birth clusters of</stro
Page 158 and 159:
from their dam, se
Page 160 and 161:
#88 Influence of b
Page 162 and 163:
#104 Relation between faecal shedde
Page 164 and 165:
Epidemiology Poster Abstracts 109 1
Page 166 and 167:
#22 The Prevalence of</stro
Page 168 and 169:
CONCLUSION IS1311-based nested Ma/M
Page 170 and 171:
#42 Seroprevalence survey o
Page 172 and 173:
#77 Characterisation of</st
Page 174 and 175:
Environmental samples were collecte
Page 176 and 177:
REFERENCES 1) Chiodini RJ, Van Krui
Page 178 and 179:
#113 Effect of soi
Page 180 and 181:
#136 Error-adjusted, variance parti
Page 182 and 183:
Latium Region Viterbo districts RES
Page 184 and 185:
#144 The suitability of</st
Page 186 and 187: #178 Age structure of</stro
Page 188 and 189: A statistically significant lower f
Page 190 and 191: #183 Sero-prevalence of</st
Page 192 and 193: #218 Johne’s disease in t
Page 194 and 195: #244 A survey to estimate t
Page 196 and 197: Control Programs Keynote Lecture Re
Page 198 and 199: #202 Milk quality assurance for par
Page 200 and 201: #141 Control of pa
Page 202 and 203: #198 Use of small
Page 204 and 205: Control Programs Poster Abstracts 1
Page 206 and 207: RESULTS Loss of al
Page 208 and 209: #40 Managing Bovine Johnes Disease
Page 210 and 211: #74 Economic analysis of</s
Page 212 and 213: #87 Evaluation of
Page 214 and 215: separately for each existing herd <
Page 216 and 217: #98 An inter-laboratory ring-trial
Page 218 and 219: #120 Paratuberculosis vaccine inter
Page 220 and 221: The statement provides: • A stand
Page 222 and 223: COMPLEMENTARY ASPECTS On-Farm Disea
Page 224 and 225: #168 Paratuberculosis in Iran: past
Page 226 and 227: #180 Behavioural change in owners <
Page 228 and 229: the fact that <str
Page 230 and 231: #182 An integrated risk based appro
Page 232 and 233: etween 0 to 10, depending on <stron
Page 234 and 235: #203 Milk quality assurance for par
Page 238 and 239: Pathogenomics and MAP Biology Conve
Page 240 and 241: #45 Identification of</stro
Page 242 and 243: #117 Adsorption of
Page 244 and 245: #222 Atypical structural features <
Page 246 and 247: Pathogenomics and MAP Biology Persp
Page 248 and 249: 247
Page 250 and 251: #52 Is ‘Indian Bison Type’ Myco
Page 252 and 253: #69 In vitro susceptibility <strong
Page 254 and 255: #118 Cloning, expression and purifi
Page 256 and 257: #176 A reference proteomic pr<stron
Page 258 and 259: #213 Construction the</stro
Page 260 and 261: #230 Iron concentration dependent s
Page 262 and 263: 261
Page 264 and 265: 263
Page 266 and 267: Abstract not available at press tim
Page 268 and 269: #174 Sharing of
Page 270 and 271: #177 Associations between CARD15 po
Page 272 and 273: Perspectives Talk: Public Health My
Page 274 and 275: 273
Page 276 and 277: A total of 206 qua
Page 278 and 279: Feller, M., K. Huwiler, R. Stephan,
Page 280 and 281: In 2008, the Ameri
Page 282 and 283: #115 Crohn’s disease and ruminant
Page 284 and 285: International John
Page 286 and 287:
#76 Towards improved reporting stan
Page 288 and 289:
#97 The EU ParaTBTools Project - Th
Page 290 and 291:
#241 US Vaccine Initiative through
Page 292 and 293:
Paratuberculosis ELISA Reliable, si
Page 294 and 295:
PRIONICS AG WAGISTRASSE 27A PHONE +
show all

Proceedings of the 10th International Colloquium on Paratuberculosis

Create successful ePaper yourself

Delete template?

Save as template?